公开(公告)号：US20240272148A1

公开(公告)日：2024-08-15

申请号：US18437875

申请日：2024-02-09

申请人： McMaster University

发明人： Carlos Filipe ,  John D. Brennan ,  Robert Pelton ,  Sana Jahanshahi-Anbuhi ,  Yingfu Li

IPC分类号： G01N33/543 ,  C12Q1/66 ,  C12Q1/686 ,  G01N21/77 ,  G01N21/78 ,  G01N33/548

CPC分类号： G01N33/5436 ,  C12Q1/66 ,  C12Q1/686 ,  G01N21/78 ,  G01N33/548 ,  G01N2021/7786 ,  G01N2333/922

摘要： The present application is directed to methods of performing chemical reactions, including multi-step chemical reactions in which two or more of the reagents in the chemical reaction are incorporated or entrapped in a solid polymeric structure comprising pullulan. In certain embodiments, the chemical reaction or multi-step reaction serves as a sensor. Accordingly the present application is also directed to sensors for performing the methods of the application. In certain embodiments, at least one of the reagents is a biomolecule and the sensor is a biosensor. In certain other embodiments, the solid polymeric structure comprising pullulan and the reagents for performing a chemical reaction form a convenient device for performing a chemical reaction.

公开(公告)号：US11549945B2

公开(公告)日：2023-01-10

申请号：US16875707

申请日：2020-05-15

申请人： McMaster University

发明人： John D. Brennan ,  Yingfu Li ,  Monsur Ali ,  Carlos Filipe ,  Michael Gregory Wolfe

IPC分类号： C12Q1/6816 ,  G01N33/569 ,  G01N33/543

摘要： Provided herein are nucleic acid-cleaving catalytic nucleic acid probes, biosensors and lateral flow biosensor devices and methods and kits of using the probes, biosensors and lateral flow biosensor devices for detecting an analyte present on or generated from a microorganism in a test sample, including Helicobacter pylori and methods for determining whether a subject has a Helicobacter pylori infection.

公开(公告)号：US20210262013A1

公开(公告)日：2021-08-26

申请号：US17180337

申请日：2021-02-19

申请人： McMaster University

发明人： Carlos Filipe ,  Yingfu Li ,  Sahar Esmaeili Samani ,  Dingran Chang ,  Erin M. McConnell ,  Meghan Rothenbroker

IPC分类号： C12Q1/6823

摘要： This disclosure relates to biosensors, and in particular, biosensors based on nucleic acid cleaving enzymes such as ribonucleotide-cleaving DNAzymes for the detection of analytes, and methods of use.

公开(公告)号：US10150986B2

公开(公告)日：2018-12-11

申请号：US15079418

申请日：2016-03-24

申请人： McMaster University

发明人： John D. Brennan ,  Yingfu Li ,  Carmen Carrasquilla

IPC分类号： C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6825 ,  C12Q1/6834 ,  G01N33/53 ,  C12Q1/6816

摘要： The present application relates to biosensors, for example comprising an high-molecular weight, tandem repeating, structure-switching nucleic acid aptamers (concatemeric aptamers) to rapidly create patterned sensors via, for example, inkjet printing. These concatemeric aptamer reporters remain immobilized at the point of printing through strong adsorption but retain sufficient segmental mobility to undergo structure switching and reporter signalling to provide both qualitative and quantitative detection of one or more analytes. In certain embodiments, inkjet printing allows for the patterning of internally referenced sensors with multiplexed detection, and provides a generic platform for on-demand printing of sensors even in remote locations.

公开(公告)号：US20230250430A1

公开(公告)日：2023-08-10

申请号：US18010878

申请日：2021-06-15

申请人： McMaster University

发明人： John Brennan ,  Yingfu Li ,  Jimmy Gu

IPC分类号： C12N15/113 ,  C12Q1/6825 ,  C12Q1/70 ,  C12Q1/6883

CPC分类号： C12N15/1131 ,  C12Q1/6825 ,  C12Q1/701 ,  C12Q1/6883 ,  C12Q2600/118

摘要： This disclosure relates to recognition moieties, biosensors, biosensor systems and kits thereof, and the methods for their use in detecting a target nucleic acid molecule in a test sample, including viral RNA and methods for determining whether a subject has a viral infection. The methods disclosed herein include detecting a viral infection in a subject comprising testing a sample from the subject for the presence of a target nucleic acid using a biosensor system, wherein presence of a target nucleic acid indicates that the subject has a viral infection.

公开(公告)号：US20220317123A1

公开(公告)日：2022-10-06

申请号：US17705000

申请日：2022-03-25

申请人： McMaster University

发明人： Yingfu Li ,  Meghan Rothenbroker ,  Erin M. McConnell ,  Carlos D. M. Filipe ,  Jimmy Gu

IPC分类号： G01N33/569 ,  C12N15/113 ,  G01N33/542

摘要： This disclosure relates to DNAzymes and biosensors for detecting pathogenic bacteria, and in particular, for detecting Legionella pneumophila. This disclosure also provides a method for detecting the presence of Legionella pneumophila in a test sample, comprising: a) contacting said test sample with the DNAzyme or biosensor described herein, wherein the DNAzyme comprises a detectable label; b) allowing cleavage of the DNAzyme if a target is present, thereby releasing the detectable label; and c) measuring a detectable signal if the portion of the DNAzyme comprising the detectable label is released, wherein the RNA cleavage activity of the DNAzyme is activated by a target from Legionella pneumophila.

公开(公告)号：US11067569B2

公开(公告)日：2021-07-20

申请号：US16274616

申请日：2019-02-13

申请人： McMaster University

发明人： Carlos Filipe ,  John D. Brennan ,  Robert Pelton ,  Sana Jahanshahi-Anbuhi ,  Yingfu Li

IPC分类号： G01N33/543 ,  G01N21/78 ,  G01N33/548 ,  C12Q1/66 ,  C12Q1/686 ,  G01N21/77

摘要： The present application is directed to methods of performing chemical reactions, including multi-step chemical reactions in which two or more of the reagents in the chemical reaction are incorporated or entrapped in a solid polymeric structure comprising pullulan. In certain embodiments, the chemical reaction or multi-step reaction serves as a sensor. Accordingly the present application is also directed to sensors for performing the methods of the application. In certain embodiments, at least one of the reagents is a biomolecule and the sensor is a biosensor. In certain other embodiments, the solid polymeric structure comprising pullulan and the reagents for performing a chemical reaction form a convenient device for performing a chemical reaction.

公开(公告)号：US10241109B2

公开(公告)日：2019-03-26

申请号：US15034914

申请日：2014-11-10

申请人： McMaster University

发明人： Carlos Filipe ,  John D. Brennan ,  Robert Pelton ,  Sana Jahanshahi-Anbuhi ,  Yingfu Li

IPC分类号： G01N33/543 ,  G01N33/548 ,  G01N21/78 ,  C12Q1/66 ,  C12Q1/68 ,  C12Q1/686 ,  G01N21/77

摘要： The present application is directed to methods of performing chemical reactions, including multi-step chemical reactions in which two or more of the reagents in the chemical reaction are incorporated or entrapped in a solid polymeric structure comprising pullulan. In certain embodiments, the chemical reaction or multi-step reaction serves as a sensor. Accordingly the present application is also directed to sensors for performing the methods of the application. In certain embodiments, at least one of the reagents is a biomolecule and the sensor is a biosensor. In certain other embodiments, the solid polymeric structure comprising pullulan and the reagents for performing a chemical reaction form a convenient device for performing a chemical reaction.

公开(公告)号：US20190071714A1

公开(公告)日：2019-03-07

申请号：US16121120

申请日：2018-09-04

申请人： McMaster University

发明人： Yingfu Li ,  John D. Brennan ,  Meng Liu

IPC分类号： C12Q1/689 ,  C12N15/10

摘要： Provided herein are aptamers, aptamer probes and biosensor systems that detect C. difficile glutamate dehydrogenase (GDH). Also provided are methods of detecting C. difficile GDH using the aptamers, probes and biosensors and methods of determining whether a subject has a C. difficile infection.

公开(公告)号：US20160281145A1

公开(公告)日：2016-09-29

申请号：US15079418

申请日：2016-03-24

申请人： McMaster University

发明人： John D. Brennan ,  Yingfu Li ,  Carmen Carrasquilla

IPC分类号： C12Q1/68 ,  G01N33/53

CPC分类号： C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  G01N33/5308 ,  C12Q2525/205 ,  C12Q2565/518 ,  C12Q2565/607

摘要： The present application relates to biosensors, for example comprising an high-molecular weight, tandem repeating, structure-switching nucleic acid aptamers (concatemeric aptamers) to rapidly create patterned sensors via, for example, inkjet printing. These concatemeric aptamer reporters remain immobilized at the point of printing through strong adsorption but retain sufficient segmental mobility to undergo structure switching and reporter signalling to provide both qualitative and quantitative detection of one or more analytes. In certain embodiments, inkjet printing allows for the patterning of internally referenced sensors with multiplexed detection, and provides a generic platform for on-demand printing of sensors even in remote locations.

摘要翻译： 本申请涉及生物传感器，例如包括通过例如喷墨印刷快速形成图案化传感器的高分子量串联重复结构转换核酸适配体（并用适体）。 这些连接适体报告者通过强吸附保持固定在印刷点，但保留足够的分段迁移率进行结构切换和报告信号，以提供一种或多种分析物的定性和定量检测。 在某些实施例中，喷墨打印允许通过多路检测对内部参考的传感器进行图案化，并且为即使在偏远位置的传感器的按需打印提供通用平台。