公开(公告)号：US20130266600A1

公开(公告)日：2013-10-10

申请号：US13742539

申请日：2013-01-16

申请人： Medimmune, LLC

发明人： Hong Jin ,  Robert Brazas ,  Bin Lu

IPC分类号： C12N7/04

CPC分类号： C12N7/045 ,  A61K39/12 ,  A61K39/155 ,  A61K2039/5254 ,  A61K2039/543 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2760/18522 ,  C12N2760/18534 ,  C12N2760/18543 ,  C12N2760/18561 ,  C12N2800/40 ,  G01N33/56983 ,  G01N2469/20 ,  G01N2500/00

摘要： The present invention provides recombinant respiratory syncytial viruses that have an attenuated phenotype and that comprise one or more mutations in the viral P, M2-1 and/or M2-2 proteins, as well as live attenuated vaccines comprising such viruses and nucleic acids encoding such viruses. Recombinant RSV P, M2-1 and M2-2 proteins are described. Methods of producing attenuated recombinant RSV, and methods of quantitating neutralizing antibodies that utilize recombinant viruses of family Paramyxoviridae, are also provided.

公开(公告)号：US20140220075A1

公开(公告)日：2014-08-07

申请号：US14222461

申请日：2014-03-21

申请人： MEDIMMUNE, LLC

发明人： Enrich HOFFMAN ,  Hong Jin ,  Bin Lu ,  Greg Duke ,  George Kemble

IPC分类号： A61K39/145

CPC分类号： A61K39/145 ,  A61K2039/5254 ,  C07K14/005 ,  C12N7/00 ,  C12N15/85 ,  C12N15/86 ,  C12N15/87 ,  C12N2760/16122 ,  C12N2760/16143 ,  C12N2760/16162 ,  C12N2760/16222 ,  C12N2760/16243 ,  C12N2760/16262

摘要： Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided.

摘要翻译： 提供了用于在细胞培养中生产适合作为重组流感疫苗的流感病毒的载体和方法。 提供了用于多质粒流感病毒表达系统的双向表达载体。