公开(公告)号：US20050037420A1

公开(公告)日：2005-02-17

申请号：US10489328

申请日：2002-09-13

申请人： Mei-Yun Zhang ,  Stefan Schillberg ,  Sabine Zimmermann ,  Stefano Fiore ,  Neil Emans ,  Rainer Fischer

发明人： Mei-Yun Zhang ,  Stefan Schillberg ,  Sabine Zimmermann ,  Stefano Fiore ,  Neil Emans ,  Rainer Fischer

IPC分类号： C07K16/00 ,  C07K16/08 ,  G01N33/53 ,  C07K16/44

CPC分类号： C07K16/081 ,  A01K2217/05 ,  C07K16/00 ,  C07K2317/13 ,  C07K2317/23 ,  C07K2317/24 ,  C07K2317/567 ,  C07K2317/622

摘要： This invention relates to immunoglobulin molecules comprising light chain (VL) chimeric variable domains, heavy chain (VH) chimeric variable domains, e.g., scFv antibodies that are expressed at high levels within a host cell, preferably within particular cellular compartments such as, e.g., cytosol or apoplast. The VL, VH and scFv antibody molecules comprise framework scaffolds of particularly preferred framework regions. This invention also relates to nucleic acid molecules encoding the immunoglobulin molecules of this invention, vectors expressing the immunoglobulin molecules, hosts transformed with the nucleic acid molecules and vectors, and methods of using the immunoglobulin molecules. Also described are immunoglobulin libraries as well as host cells, including transgenic plants, expressing the VL, VH or scFv antibody molecules of this invention.

摘要翻译： 本发明涉及包含轻链（VL）嵌合可变域，重链（VH）嵌合可变结构域的免疫球蛋白分子，例如在宿主细胞内，优选在特定细胞区室内以高水平表达的scFv抗体，例如， 胞质或质外体。 VL，VH和scFv抗体分子包括特别优选的框架区的框架支架。 本发明还涉及编码本发明的免疫球蛋白分子的核酸分子，表达免疫球蛋白分子的载体，用核酸分子和载体转化的宿主，以及使用免疫球蛋白分子的方法。 还描述了免疫球蛋白文库以及表达本发明的VL，VH或scFv抗体分子的宿主细胞，包括转基因植物。