公开(公告)号：US08202723B2

公开(公告)日：2012-06-19

申请号：US13134190

申请日：2011-05-31

申请人： Menzo Jans Emco Havenga ,  Ronald Vogels ,  Jerald C. Sadoff ,  David Hone ,  Yasir Abdul Wahid Skeiky ,  Katarina Rado{hacek over (s)}evic

发明人： Menzo Jans Emco Havenga ,  Ronald Vogels ,  Jerald C. Sadoff ,  David Hone ,  Yasir Abdul Wahid Skeiky ,  Katarina Rado{hacek over (s)}evic

IPC分类号： C12N15/00 ,  C12N15/09 ,  C12N15/48 ,  C12N15/52 ,  C12N15/867

CPC分类号： A61K39/04 ,  A61K35/761 ,  A61K2039/523 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/55544 ,  C07K14/35 ,  C07K2319/00 ,  C12N2710/10343

摘要： Described are vaccines comprising recombinant vectors, such as recombinant adenoviruses. The vectors comprise heterologous nucleic acids encoding at least two antigens from one or more tuberculosis-causing bacilli. Also described is the use of specific protease recognition sites linking antigens through which the encoded antigens are separated upon cleavage. After cleavage, the antigens contribute to the immune response in a separate manner. The recombinant vectors may comprise a nucleic acid encoding the protease cleaving the linkers and separating the antigens. Also described is the use of genetic adjuvants encoded by the recombinant vectors, wherein such genetic adjuvants may also be cleaved through the presence of the cleavable linkers and the specific protease.

摘要翻译： 描述了包含重组载体例如重组腺病毒的疫苗。 载体包含编码来自一种或多种结核分枝杆菌的至少两种抗原的异源核酸。 还描述了连接抗原的特异性蛋白酶识别位点的使用，通过该抗原，编码的抗原在切割时被分离。 在切割后，抗原以单独的方式有助于免疫应答。 重组载体可以包含编码切割接头并分离抗原的蛋白酶的核酸。 还描述了由重组载体编码的遗传佐剂的用途，其中这种遗传佐剂也可以通过可切割接头和特异性蛋白酶的存在来切割。

公开(公告)号：US08609402B2

公开(公告)日：2013-12-17

申请号：US13134206

申请日：2011-05-31

申请人： Menzo Jans Emco Havenga ,  Ronald Vogels ,  Jerald C. Sadoff ,  David Hone ,  Yasir Abdul Wahid Skeiky ,  Katarina Rado{hacek over (s)}evic

发明人： Menzo Jans Emco Havenga ,  Ronald Vogels ,  Jerald C. Sadoff ,  David Hone ,  Yasir Abdul Wahid Skeiky ,  Katarina Rado{hacek over (s)}evic

IPC分类号： C12N15/00 ,  C12N15/09 ,  C12N15/48 ,  C12N15/52 ,  C12N15/867

CPC分类号： A61K39/04 ,  A61K35/761 ,  A61K2039/523 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/55544 ,  C07K14/35 ,  C07K2319/00 ,  C12N2710/10343

摘要： The invention relates to vaccines comprising recombinant vectors, such as recombinant adenoviruses. The vectors comprise heterologous nucleic acids encoding for at least two antigens from one or more tuberculosis-causing bacilli. Also described is the use of specific protease recognition sites linking antigens through which the encoded antigens are separated upon cleavage. After cleavage, the antigens contribute to the immune response in a separate manner. The recombinant vectors may comprise a nucleic acid encoding the protease cleaving the linkers and separating the antigens. Further described is the use of genetic adjuvants encoded by the recombinant vectors, wherein such genetic adjuvants may also be cleaved through the presence of the cleavable linkers and the specific protease.

摘要翻译： 本发明涉及包含重组载体例如重组腺病毒的疫苗。 载体包含编码来自一种或多种结核分枝杆菌的至少两种抗原的异源核酸。 还描述了连接抗原的特异性蛋白酶识别位点的使用，通过该抗原，编码的抗原在切割时被分离。 在切割后，抗原以单独的方式有助于免疫应答。 重组载体可以包含编码切割接头并分离抗原的蛋白酶的核酸。 进一步描述了由重组载体编码的遗传佐剂的用途，其中此类遗传佐剂也可通过存在可切割连接子和特异性蛋白酶来切割。