公开(公告)号：US20170044588A1

公开(公告)日：2017-02-16

申请号：US15305703

申请日：2015-03-30

Applicant: Merck Patent GmbH

Inventor： Luc FELDEN ,  Marine BOUTHILLON ,  Stephane OLIVIER ,  Pierre WOEHL ,  Pierre GUEDON ,  Florian ALLARD

IPC: C12Q1/04 ,  G01N21/47 ,  C12Q1/22 ,  G01N21/55 ,  G06K9/00 ,  G06T7/00

CPC classification number: C12Q1/04 ,  C12Q1/22 ,  G01N21/47 ,  G01N21/55 ,  G01N2021/1765 ,  G01N2021/4704 ,  G01N2021/4709 ,  G01N2021/4711 ,  G01N2201/103 ,  G06K9/00134 ,  G06T7/0012 ,  G06T2207/10012 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: The present invention is directed to a method and an apparatus for detecting micro-colonies growing on a membrane or an agarose medium of a sample in a closed device. According to the invention the sample is irradiated with a light incident at an angle (β) with respect to the normal to the membrane or the surface of the agarose medium from outside the device. An incident area of the light on the membrane or the surface of the agarose medium is imaged by means of a light receiving element using an imaging angle (α) different from angle (β) with respect to the normal to the membrane or the surface of the agarose medium from outside the device. The light reflected, scattered and/or diffused from the membrane or the surface of the agarose medium and/or the micro-colonies on the membrane and/or the micro-colonies on the agarose medium is detected.

Abstract translation: 本发明涉及一种用于检测在封闭装置中在样品的膜或琼脂糖培养基上生长的微菌落的方法和装置。 根据本发明，从设备外部以相对于膜的法线或琼脂糖介质的表面以角度（β）入射的光照射样品。 使用不同于角度（β）的成像角（α）相对于膜的法线或表面的受光元件使膜的膜或琼脂糖介质的表面上的光的入射面积成像 琼脂糖介质从设备外部。 检测从膜或琼脂糖介质的表面和/或膜上的微生物群和/或琼脂糖培养基上的微菌落反射，散射和/或扩散的光。

公开(公告)号：US20180349671A1

公开(公告)日：2018-12-06

申请号：US15760900

申请日：2016-08-17

Applicant: MERCK PATENT GMBH ,  UNIVERSITE DE STRASBOURG ,  Centre National de la Recherche Scientifique (CNRS)

Inventor： Marine BOUTHILLON ,  Luc FELDEN ,  Ola AHMAD ,  Christophe COLLET

IPC: G06K9/00 ,  C12Q1/06 ,  G06K9/40

CPC classification number: C12Q1/06 ,  G06K9/0014 ,  G06K9/00147 ,  G06K9/46 ,  G06T7/0016 ,  G06T2207/10028 ,  G06T2207/20076 ,  G06T2207/30024

Abstract: The present invention is directed to a method for detection of microbial colonies on a surface, comprising the steps of obtaining one or a plurality of digital images I0, I1, I2, I3, I4 of the surface, said digital images I0, I1, I2, I3, I4 being represented by at least two-dimensional matrices of pixel values, calculating a statistical noise distribution based on at least one of the digital images I0, applying the statistical noise distribution calculated in the calculation step to the one or the plurality of digital images I0, I1, I2, I3, I4, and detecting an object of interest as a candidate for a microbial colony based on deviation of pixel values from the noise distribution.