公开(公告)号：US07748283B2

公开(公告)日：2010-07-06

申请号：US11707313

申请日：2007-02-16

申请人： Michael A. Harvey ,  Breck O. Parker ,  Stevan P. Tortorella ,  Elizabeth A Moran ,  John Pipinias

发明人： Michael A. Harvey ,  Breck O. Parker ,  Stevan P. Tortorella ,  Elizabeth A Moran ,  John Pipinias

IPC分类号： B01L3/00

CPC分类号： B01L9/00 ,  B01L3/5023 ,  B01L3/505 ,  B01L2200/027 ,  B01L2300/069 ,  G01N1/02 ,  G01N2001/028 ,  G01N2035/00356

摘要： The field of the present invention pertains to a controlled transfer biological collection device using a dry solid storage and transfer medium and a method for the collection of biological material of interest (genetic or proteinaceous material) in a form suitable for storage and/or subsequent analysis. Specifically, the present invention provides for a sampling device that controls the transfer of the biological sample to the storage medium by holding the storage medium and a moveable sample collection member having an analyte collection surface. The invention further provides for a method not only for storing a biological analyte on this collection device but also for analyzing the stored biological analyte using methods that are suited for automated analyzing systems.

摘要翻译： 本发明的领域涉及使用干燥固体储存和转移介质的受控转移生物收集装置和用于以适于储存和/或随后分析的形式收集目的生物材料（遗传或蛋白质材料）的方法 。 具体地，本发明提供一种采样装置，其通过保持存储介质和具有分析物收集表面的可移动样品收集构件来控制生物样品向存储介质的转移。 本发明还提供了不仅用于将生物分析物储存在该收集装置上的方法，而且还用于使用适合于自动分析系统的方法分析储存的生物分析物的方法。

公开(公告)号：US20080196517A1

公开(公告)日：2008-08-21

申请号：US11707313

申请日：2007-02-16

申请人： Michael A. Harvey ,  Breck O. Parker ,  Stevan P. Tortorella ,  Elizabeth A. Moran ,  John Pipinias

发明人： Michael A. Harvey ,  Breck O. Parker ,  Stevan P. Tortorella ,  Elizabeth A. Moran ,  John Pipinias

IPC分类号： G01N1/00 ,  B01L3/00

CPC分类号： B01L9/00 ,  B01L3/5023 ,  B01L3/505 ,  B01L2200/027 ,  B01L2300/069 ,  G01N1/02 ,  G01N2001/028 ,  G01N2035/00356

摘要： The field of the present invention pertains to a controlled transfer biological collection device using a dry solid storage and transfer medium and a method for the collection of biological material of interest (genetic or proteinaceous material) in a form suitable for storage and/or subsequent analysis. Specifically, the present invention provides for a sampling device that controls the transfer of the biological sample to the storage medium by holding the storage medium and a moveable sample collection member having an analyte collection surface. The invention further provides for a method not only for storing a biological analyte on this collection device but also for analyzing the stored biological analyte using methods that are suited for automated analyzing systems.

摘要翻译： 本发明的领域涉及使用干燥固体储存和转移介质的受控转移生物收集装置和用于以适于储存和/或随后分析的形式收集目的生物材料（遗传或蛋白质材料）的方法 。 具体地，本发明提供一种采样装置，其通过保持存储介质和具有分析物收集表面的可移动样品收集构件来控制生物样品向存储介质的转移。 本发明还提供了不仅用于将生物分析物储存在该收集装置上的方法，而且还用于使用适合于自动分析系统的方法分析储存的生物分析物的方法。

公开(公告)号：US20100106057A1

公开(公告)日：2010-04-29

申请号：US12449603

申请日：2008-02-15

申请人： Michael A. Harvey ,  Breck O. Parker ,  Stevan Paul Tortorella ,  Elizabeth A. Moran ,  John Pipinias

发明人： Michael A. Harvey ,  Breck O. Parker ,  Stevan Paul Tortorella ,  Elizabeth A. Moran ,  John Pipinias

IPC分类号： A61B10/00

CPC分类号： B01L9/00 ,  B01L3/5023 ,  B01L3/505 ,  B01L2200/027 ,  B01L2300/069 ,  G01N1/02 ,  G01N2001/028 ,  G01N2035/00356

摘要： The field of the present invention pertains to a controlled transfer biological collection device using a dry solid storage and transfer medium and a method for the collection of biological material of interest (genetic or proteinaceous material) in a form suitable for storage and/or subsequent analysis. Specifically, the present invention provides for a sampling device that controls the transfer of the biological sample to the storage medium by holding the storage medium and a moveable sample collection member having an analyte collection surface. The invention further provides for a method not only for storing a biological analyte on this collection device but also for analyzing the stored biological analyte using methods that are suited for automated analyzing systems.

摘要翻译： 本发明的领域涉及使用干燥固体储存和转移介质的受控转移生物收集装置和用于以适于储存和/或随后分析的形式收集目的生物材料（遗传或蛋白质材料）的方法 。 具体地，本发明提供一种采样装置，其通过保持存储介质和具有分析物收集表面的可移动样品收集构件来控制生物样品向存储介质的转移。 本发明还提供了不仅用于将生物分析物储存在该收集装置上的方法，而且还用于使用适合于自动分析系统的方法分析储存的生物分析物的方法。

公开(公告)号：US08468901B2

公开(公告)日：2013-06-25

申请号：US12449603

申请日：2008-02-15

申请人： Michael A. Harvey ,  Breck O. Parker ,  Stevan Paul Tortorella ,  Elizabeth A. Moran ,  John Pipinias

发明人： Michael A. Harvey ,  Breck O. Parker ,  Stevan Paul Tortorella ,  Elizabeth A. Moran ,  John Pipinias

IPC分类号： B01L3/00

CPC分类号： B01L9/00 ,  B01L3/5023 ,  B01L3/505 ,  B01L2200/027 ,  B01L2300/069 ,  G01N1/02 ,  G01N2001/028 ,  G01N2035/00356

摘要： The field of the present invention pertains to a controlled transfer biological collection device using a dry solid storage and transfer medium and a method for the collection of biological material of interest (genetic or proteinaceous material) in a form suitable for storage and/or subsequent analysis. Specifically, the present invention provides for a sampling device that controls the transfer of the biological sample to the storage medium by holding the storage medium and a moveable sample collection cmember having an analyte collection surface. The invention further provides for a method not only for storing a biological analyte on this collection device but also for analyzing the stored biological analyte using methods that are suited for automated analyzing systems.

摘要翻译： 本发明的领域涉及使用干燥固体储存和转移介质的受控转移生物收集装置和用于以适于储存和/或随后分析的形式收集目的生物材料（遗传或蛋白质材料）的方法 。 具体地，本发明提供一种采样装置，其通过保持存储介质和具有分析物收集表面的可移动的样品收集组件来控制生物样品向存储介质的转移。 本发明还提供了不仅用于将生物分析物储存在该收集装置上的方法，而且还用于使用适合于自动分析系统的方法分析储存的生物分析物的方法。

公开(公告)号：US06352827B1

公开(公告)日：2002-03-05

申请号：US08908494

申请日：1997-08-07

申请人： Edmund C. C. Lin ,  Breck O. Parker

发明人： Edmund C. C. Lin ,  Breck O. Parker

IPC分类号： C12Q170

CPC分类号： C12Q1/70 ,  C12Q1/6823 ,  Y10T436/143333 ,  C12Q2565/519 ,  C12Q2537/113 ,  C12Q2521/301 ,  C12Q2563/149

摘要： The present invention provides methods and kits for detection, and optionally quantitation, of multiple nucleic acid sequence analytes in a single fluid sample. The assays and kits of the invention employ a plurality of species of capture probes, each species comprising a bacteriophage covalently or non-covalently linked to an oligonucleotide complementary to one analyte, wherein the bacteriophage of each species is capable of generating a distinctive signal when plated on an indicator medium. The oligonucleotide moieties of each capture probe contain a sufficient number of contiguous ribonucleotides to confer sensitivity to a ribonuclease specific for RNA/DNA or RNA/RNA duplexes. In the method of the invention, the capture probe species are combined with the fluid sample, which has been processed to release single-stranded nucleic acids. Hybridization is allowed to occur, thereby generating DNA/RNA or RNA/RNA duplexes. By adding a nucleolytic enzyme capable of cleaving DNA-RNA duplexes, bacteriophage are released for detection of the different signals on an indicator medium. The kit of the invention provides components which allow the method of the invention to be performed.

摘要翻译： 本发明提供用于在单个流体样品中检测和任选定量多个核酸序列分析物的方法和试剂盒。 本发明的测定和试剂盒使用多种捕获探针，每种物质包含与一种分析物互补的寡核苷酸共价或非共价连接的噬菌体，其中每种物种的噬菌体在电镀时能够产生独特的信号 在指示剂介质上。 每个捕获探针的寡核苷酸部分含有足够数量的连续核糖核苷酸以赋予对RNA / DNA或RNA / RNA双链体特异的核糖核酸酶的敏感性。 在本发明的方法中，将捕获探针物质与经过处理以释放单链核酸的流体样品组合。 允许发生杂交，从而产生DNA / RNA或RNA / RNA双链体。 通过添加能够切割DNA-RNA双链体的核解质酶，释放噬菌体以检测指示剂介质上的不同信号。 本发明的试剂盒提供允许本发明方法进行的组分。