公开(公告)号：US5053226A

公开(公告)日：1991-10-01

申请号：US73500

申请日：1987-07-15

申请人： Michael G. Rosenblum ,  James L. Murray ,  Peter J. Kelleher ,  Robert A. Newman ,  Abdul R. Khokhar

发明人： Michael G. Rosenblum ,  James L. Murray ,  Peter J. Kelleher ,  Robert A. Newman ,  Abdul R. Khokhar

IPC分类号： A61K38/00 ,  A61K47/48 ,  C07K16/44 ,  C07K16/46

CPC分类号： C07K16/44 ,  A61K47/48676 ,  C07K16/468 ,  A61K38/00

摘要： The present invention relates to monoclonal antibodies specifically binding platinum (II) complex. The monoclonal antibodies of the present invention are characteristically produced by antibody-producing cell lines such as hybridomas. These cell lines may result, for example, from fusion of a neoplastic cell with an antibody-producing animal cell obtained from an animal immunized against a platinum (II) complex. The cellular fusion products include cell lines forming monoclonal antibody specifically binding platinum (II) complex in competition with an antibody produced by: hybridoma strain 1C.sub.1 H.sub.2 A.sub.5, deposited with the American Type Culture Collection, Rockville, MD on May 1, 1987 and having ATCC accession number HB 9411; strain 3A.sub.2 A.sub.1 ; strain 1A.sub.6 A.sub.2 ; strain 3A.sub.6 B.sub.1 or strain 1B.sub.1. The cell lines of the present invention are preferably continuous murine hybridoma cell lines which secrete recoverable quantities of monoclonal antibody, particularly a monoclonal antibody which is of an IgG or IgM isotype.

摘要翻译： 本发明涉及特异性结合铂（II）复合物的单克隆抗体。 本发明的单克隆抗体的特征在于产生抗体的细胞系如杂交瘤。 这些细胞系可以例如来自肿瘤细胞与获自抗铂（II）复合物的动物获得的产生抗体的动物细胞的融合。 细胞融合产物包括形成特异性结合铂（II）复合物的单克隆抗体的细胞系，与1987年5月1日保藏于美国典型培养物保藏中心，Rockville，MD的杂交瘤菌株1C1H2A5产生的抗体竞争，并具有ATCC登录号 HB 9411; 菌株3A2A1; 菌株1A6A2; 菌株3A6B1或菌株1B1。 本发明的细胞系优选分泌可回收量的单克隆抗体，特别是IgG或IgM同种型的单克隆抗体的连续鼠杂交瘤细胞系。

公开(公告)号：US07348153B2

公开(公告)日：2008-03-25

申请号：US11286917

申请日：2005-11-23

申请人： Soldano Ferrone ,  Constantin Ioannides ,  James L. Murray ,  Kouichiro Kawano

发明人： Soldano Ferrone ,  Constantin Ioannides ,  James L. Murray ,  Kouichiro Kawano

IPC分类号： G01N33/53

CPC分类号： G01N33/686 ,  G01N33/6863 ,  G01N33/6878

摘要： The invention provides a method for identifying peptides for use in increasing a cytotoxic T lymphocytes (CTL) response to an antigen. The method comprises the steps of comparing the amino acid sequence of the VH and/or VL portions of an anti-idiotypic monoclonal antibody to the amino acid sequence of an antigen to identify regions of homology between the Ab2 and the antigen, and to further identify an HLA binding motif in a homologous region. The identified homologous region which comprises an HLA binding motif defines a peptide sequence that is useful for stimulating a CTL response. Also provided are peptides identified by the method, and a method of using the peptides to stimulate a CTL response in an individual.

摘要翻译： 本发明提供了一种用于鉴定用于增加对抗原的细胞毒性T淋巴细胞（CTL）应答的肽的方法。 该方法包括将抗独特型单克隆抗体的VH部分和/或VL部分的氨基酸序列与抗原的氨基酸序列进行比较的步骤，以鉴定Ab2与抗原之间的同源性区域，并进一步鉴定 同源区的HLA结合基序。 鉴定的包含HLA结合基序的同源区定义了可用于刺激CTL应答的肽序列。 还提供了通过该方法鉴定的肽以及使用肽刺激个体中CTL应答的方法。

公开(公告)号：US20160010152A1

公开(公告)日：2016-01-14

申请号：US14866185

申请日：2015-09-25

申请人： David A. Shafer ,  James L. Murray ,  Peixu Hu

发明人： David A. Shafer ,  James L. Murray ,  Peixu Hu

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6876 ,  C12Q1/6883 ,  C12Q2600/16

摘要： Provided herein are multi-probe systems and methods for amplifying and/or detecting multiple nucleic acid targets in a sample. The multi-probe systems comprise two or more probes having identical and/or different labels and are used together to target two or more related signature sequences in a genus, a species, or a gene. The probes comprise one or more probe:antiprobe systems selected from iDDS probes, iDDS-2Q probes, MacMan probes, Flip probes, Universal probes, Half-Universal probes, ZIPR probes, and G-Force probes. The probes are generally flanked by one set of primers, one primer, one primer-probe, or two primers. The methods utilize the multi-probe systems in real time PCR analysis for facilitating the assessment and/or diagnosis of nucleic acid sequences by providing confirmation of target detection and/or by providing greater signaling per test.

摘要翻译： 本文提供了用于在样品中扩增和/或检测多个核酸靶的多探针系统和方法。 多探针系统包含具有相同和/或不同标记的两个或更多个探针，并且一起用于靶基因，物种或基因中的两个或多个相关特征序列。 探针包括一个或多个探针：选自iDDS探针，iDDS-2Q探针，MacMan探针，Flip探针，通用探针，半通用探针，ZIPR探针和G-Force探针的探针。 探针一般侧翼为一组引物，一个引物，一个引物 - 探针或两个引物。 该方法利用多探针系统进行实时PCR分析，以通过提供目标检测的确认和/或通过每个测试提供更大的信号来促进核酸序列的评估和/或诊断。