利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

12 条记录 (耗时 0.023 秒)

    Probe:antiprobe compositions for high specificity DNA or RNA detection
    2.
    发明授权
    Probe:antiprobe compositions for high specificity DNA or RNA detection 有权

    公开(公告)号：US11667971B2

    公开(公告)日：2023-06-06

    申请号：US14216413

    申请日：2014-03-17

    申请人： David A. Shafer

    发明人： David A. Shafer

    IPC分类号： C07H21/02 ,  C12Q1/6876 ,  C12Q1/6832

    CPC分类号： C12Q1/6876 ,  C12Q1/6832 ,  C12Q1/6832 ,  C12Q2537/1373 ,  C12Q2537/161 ,  C12Q2537/163

    摘要： Probe systems and methods are provided for detecting nucleic acid targets using labeled polynucleotide probes and antiprobes that interact together and with complementary targets. These interactions result in signaling changes that indicate target frequency and provide error-checking functions that facilitate single base discrimination. These probe:antiprobe compositions enable real-time PCR detection, end-point detection and microarray detection of microbial species, drug resistant mutants, and cancer related variants. The probe:antiprobe may be an internal probe between two primers or may be a primer-probe. The probe also may be modified by introducing a base mismatch to increase thermodynamic discrimination of a correct versus incorrect target differing by a single base. Probe systems also are provided for use in methods of increasing target amplification and detecting specific single base variants.

    Probe-antiprobe compositions and methods for DNA or RNA detection
    3.
    发明申请
    Probe-antiprobe compositions and methods for DNA or RNA detection 失效
    探针 - 反扑针组合物和DNA或RNA检测方法

    公开(公告)号：US20090209434A1

    公开(公告)日：2009-08-20

    申请号：US11893323

    申请日：2007-08-15

    申请人： David A. Shafer

    发明人： David A. Shafer

    IPC分类号： C40B30/04 ,  C07H21/04 ,  C12Q1/68

    CPC分类号： C12Q1/6818 ,  C12Q2537/162 ,  C12Q2525/173 ,  C12Q2525/161 ,  C12Q2565/525 ,  C12Q2561/113 ,  C12Q2565/101 ,  C12Q2537/161 ,  C12Q2537/1373

    摘要： The invention provides novel compositions and methods for detecting unlabeled nucleic acid targets using labeled polynucleotide probes and partially complementary antiprobes. The interaction of probes, antiprobes and targets result in signaling changes that indicate target frequency. This novel detection mechanism is called a DNA detection switch, and it enable end-point detection, microarray detection and real-time PCR detection of a variety of nucleic acid targets including microbial species and subspecies, drug resistant mutants, and pathogenic strains.

    摘要翻译： 本发明提供用于使用标记的多核苷酸探针和部分互补的抗体来检测未标记的核酸靶的新的组合物和方法。 探针，对手和靶标的相互作用导致指示目标频率的信号变化。 这种新型检测机制称为DNA检测开关，可实现多种核酸靶点（包括微生物物种和亚种，耐药性突变体和致病菌株）的终点检测，微阵列检测和实时PCR检测。

    Systems and Methods to Quantify and Amplify Both Signaling and Probes for CDNA Chips and Gene Expression Microarrays
    5.
    发明申请
    Systems and Methods to Quantify and Amplify Both Signaling and Probes for CDNA Chips and Gene Expression Microarrays 审中-公开
    用于定量和扩增CDNA芯片和基因表达微阵列的信号和探针的系统和方法

    公开(公告)号：US20090275029A1

    公开(公告)日：2009-11-05

    申请号：US12334036

    申请日：2008-12-12

    申请人： David A. Shafer

    发明人： David A. Shafer

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C07H21/04 ,  C12Q1/68 ,  C12Q2521/107 ,  C12Q2525/155 ,  C12Q2525/161

    摘要： The invention provides a series of reagent compositions and methods for making and amplifying novel cDNA based probe sets from RNA samples to improve analysis with gene expression arrays. The methods globally produce probe sets with common universal linkers at one or both ends, called WRAP-Probes, wherein the linkers do not bind to the target sequences and they can efficiently bind added reporters to the probes. The universal linkers are also designed as primer binding sites for copying and amplifying the probes, either linearly with one linker, or exponentially with double linkers. The capacity to globally and exponentially amplify the probe set by PCR is a primary advantage. Adding reporters by terminal linkers also improves quantification since each probe gets equivalent signaling. The invention allows expression analysis of small research, clinical and forensic samples to enable improved diagnostics, drug discovery, therapeutic monitoring, and medical, agricultural and general research.

    摘要翻译： 本发明提供一系列用于从RNA样品制备和扩增新的基于cDNA的探针组的试剂组合物和方法，以改进基因表达阵列的分析。 这些方法全局产生探针组，其中一端或两端具有通用通用接头，称为WRAP探针，其中接头不与目标序列结合，并且它们可以有效地将添加的记录结合到探针中。 通用接头还被设计为用于复制和放大探针的引物结合位点，线性地与一个接头连接，或以双连接体指数地形成。 通过PCR全局和指数扩增探针组的能力是主要的优点。 由终端连接器添加记者也可以提高量化，因为每个探针都获得等效信号。 本发明允许小型研究，临床和法医样品的表达分析，以改进诊断，药物发现，治疗监测以及医学，农业和一般研究。

    Systems and methods to quantify and amplify both signaling probes for cDNA chips and genes expression microarrays
    6.
    发明授权
    Systems and methods to quantify and amplify both signaling probes for cDNA chips and genes expression microarrays 有权
    用于量化和扩增cDNA芯片和基因表达微阵列的信号探针的系统和方法

    公开(公告)号：US07482443B2

    公开(公告)日：2009-01-27

    申请号：US10380596

    申请日：2001-03-09

    申请人： David A. Shafer

    发明人： David A. Shafer

    IPC分类号： C07H21/04 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C07H21/04 ,  C12Q1/68 ,  C12Q2521/107 ,  C12Q2525/155 ,  C12Q2525/161

    摘要： The invention provides a series of reagent compositions and methods for making and amplifying novel cDNA based probe sets from RNA samples to improve analysis with gene expression arrays. The methods globally produce probe sets with common universal linkers at one or both ends, called WRAP-Probes, wherein the linkers do not bind to the target sequences and they can efficiently bind added reporters to the probes. The universal linkers are also designed as primer binding sites for copying and amplifying the probes, either linearly with one linker, or exponentially with double linkers. The capacity to globally and exponentially amplify the probe set by PCR is a primary advantage. Adding reporters by terminal linkers also improves quantification since each probe gets equivalent signaling. The invention allows expression analysis of small research, clinical and forensic samples to enable improved diagnostics, drug discovery, therapeutic monitoring, and medical, agricultural and general research.

    摘要翻译： 本发明提供一系列用于从RNA样品制备和扩增新的基于cDNA的探针组的试剂组合物和方法，以改进基因表达阵列的分析。 这些方法全局产生探针组，其中一端或两端具有通用通用接头，称为WRAP探针，其中接头不与目标序列结合，并且它们可以有效地将添加的记录结合到探针中。 通用接头还被设计为用于复制和放大探针的引物结合位点，线性地与一个接头连接，或以双连接体指数地形成。 通过PCR全局和指数扩增探针组的能力是主要的优点。 由终端连接器添加记者也可以提高量化，因为每个探针都获得等效信号。 本发明允许小型研究，临床和法医样品的表达分析，以改进诊断，药物发现，治疗监测以及医学，农业和一般研究。

    Dual Probe:Antiprobe Compositions for DNA and RNA Detection
    7.
    发明申请
    Dual Probe:Antiprobe Compositions for DNA and RNA Detection 审中-公开
    双探针：用于DNA和RNA检测的反接头组合物

    公开(公告)号：US20160010152A1

    公开(公告)日：2016-01-14

    申请号：US14866185

    申请日：2015-09-25

    申请人： David A. Shafer ,  James L. Murray ,  Peixu Hu

    发明人： David A. Shafer ,  James L. Murray ,  Peixu Hu

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6876 ,  C12Q1/6883 ,  C12Q2600/16

    摘要： Provided herein are multi-probe systems and methods for amplifying and/or detecting multiple nucleic acid targets in a sample. The multi-probe systems comprise two or more probes having identical and/or different labels and are used together to target two or more related signature sequences in a genus, a species, or a gene. The probes comprise one or more probe:antiprobe systems selected from iDDS probes, iDDS-2Q probes, MacMan probes, Flip probes, Universal probes, Half-Universal probes, ZIPR probes, and G-Force probes. The probes are generally flanked by one set of primers, one primer, one primer-probe, or two primers. The methods utilize the multi-probe systems in real time PCR analysis for facilitating the assessment and/or diagnosis of nucleic acid sequences by providing confirmation of target detection and/or by providing greater signaling per test.

    摘要翻译： 本文提供了用于在样品中扩增和/或检测多个核酸靶的多探针系统和方法。 多探针系统包含具有相同和/或不同标记的两个或更多个探针，并且一起用于靶基因，物种或基因中的两个或多个相关特征序列。 探针包括一个或多个探针：选自iDDS探针，iDDS-2Q探针，MacMan探针，Flip探针，通用探针，半通用探针，ZIPR探针和G-Force探针的探针。 探针一般侧翼为一组引物，一个引物，一个引物 - 探针或两个引物。 该方法利用多探针系统进行实时PCR分析，以通过提供目标检测的确认和/或通过每个测试提供更大的信号来促进核酸序列的评估和/或诊断。

    Probe:Antiprobe Compositions for High Specificity DNA or RNA Detection
    8.
    发明申请
    Probe:Antiprobe Compositions for High Specificity DNA or RNA Detection 审中-公开
    探针：用于高特异性DNA或RNA检测的抗体组合物

    公开(公告)号：US20140287410A1

    公开(公告)日：2014-09-25

    申请号：US14216413

    申请日：2014-03-17

    申请人： David A. Shafer

    发明人： David A. Shafer

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6876 ,  C12Q1/6832 ,  C12Q2537/1373 ,  C12Q2537/161 ,  C12Q2537/163

    摘要： Probe systems and methods are provided for detecting nucleic acid targets using labeled polynucleotide probes and antiprobes that interact together and with complementary targets. These interactions result in signaling changes that indicate target frequency and provide error-checking functions that facilitate single base discrimination. These probe:antiprobe compositions enable real-time PCR detection, end-point detection and microarray detection of microbial species, drug resistant mutants, and cancer related variants. The probe:antiprobe may be an internal probe between two primers or may be a primer-probe. The probe also may be modified by introducing a base mismatch to increase thermodynamic discrimination of a correct versus incorrect target differing by a single base. Probe systems also are provided for use in methods of increasing target amplification and detecting specific single base variants.

    摘要翻译： 提供了探针系统和方法，用于使用标记的多核苷酸探针和与辅助靶相互作用的对映体来检测核酸靶。 这些相互作用导致了指示目标频率的信号变化，并提供了有助于单基地辨别的错误检查功能。 这些探针：反钩组合物可实现微生物物种，耐药性突变体和癌症相关变异体的实时PCR检测，终点检测和微阵列检测。 探针：反接头可以是两个引物之间的内部探针，也可以是引物探针。 探针也可以通过引入碱基不匹配来增加对单个碱基不同的正确对不正确目标的热力学鉴别。 提供探针系统用于增加靶扩增和检测特异性单碱基变体的方法。

    System and Methods to Quantify and Amplify Both Signaling and Probes for cDNA Chips and Gene Expression Microarrays
    9.
    发明申请
    System and Methods to Quantify and Amplify Both Signaling and Probes for cDNA Chips and Gene Expression Microarrays 有权
    用于量化和扩增cDNA芯片和基因表达微阵列的信号和探针的系统和方法

    公开(公告)号：US20120157343A1

    公开(公告)日：2012-06-21

    申请号：US13403012

    申请日：2012-02-23

    申请人： David A. Shafer

    发明人： David A. Shafer

    IPC分类号： C40B30/04 ,  C12M1/40 ,  C40B40/06 ,  C12Q1/68

    CPC分类号： C07H21/04 ,  C12Q1/68 ,  C12Q2521/107 ,  C12Q2525/155 ,  C12Q2525/161

    摘要： The invention provides a series of reagent compositions and methods for making and amplifying novel cDNA based probe sets from RNA samples to improve analysis with gene expression arrays. The methods globally produce probe sets with common universal linkers at one or both ends, called WRAP-Probes, wherein the linkers do not bind to the target sequences and they can efficiently bind added reporters to the probes. The universal linkers are also designed as primer binding sites for copying and amplifying the probes, either linearly with one linker, or exponentially with double linkers. The capacity to globally and exponentially amplify the probe set by PCR is a primary advantage. Adding reporters by terminal linkers also improves quantification since each probe gets equivalent signaling. The invention allows expression analysis of small research, clinical and forensic samples to enable improved diagnostics, drug discovery, therapeutic monitoring, and medical, agricultural and general research.

    摘要翻译： 本发明提供一系列用于从RNA样品制备和扩增新的基于cDNA的探针组的试剂组合物和方法，以改进基因表达阵列的分析。 这些方法全局产生探针组，其中一端或两端具有通用的通用接头，称为WRAP探针，其中接头不与目标序列结合，并且它们可以有效地将添加的记录结合到探针中。 通用接头还被设计为用于复制和放大探针的引物结合位点，线性地与一个接头连接，或以双连接体指数地形成。 通过PCR全局和指数扩增探针组的能力是主要的优点。 由终端连接器添加记者也可以提高量化，因为每个探针都获得等效信号。 本发明允许小型研究，临床和法医样品的表达分析，以改进诊断，药物发现，治疗监测以及医学，农业和一般研究。

    Probe-antiprobe compositions and methods for DNA or RNA detection
    10.
    发明授权
    Probe-antiprobe compositions and methods for DNA or RNA detection 失效
    探针 - 反扑针组合物和DNA或RNA检测方法

    公开(公告)号：US08076067B2

    公开(公告)日：2011-12-13

    申请号：US11893323

    申请日：2007-08-15

    申请人： David A. Shafer

    发明人： David A. Shafer

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6818 ,  C12Q2537/162 ,  C12Q2525/173 ,  C12Q2525/161 ,  C12Q2565/525 ,  C12Q2561/113 ,  C12Q2565/101 ,  C12Q2537/161 ,  C12Q2537/1373

    摘要： The invention provides novel compositions and methods for detecting unlabeled nucleic acid targets using labeled polynucleotide probes and partially complementary antiprobes. The interaction of probes, antiprobes and targets result in signaling changes that indicate target frequency. This novel detection mechanism is called a DNA detection switch, and it enable end-point detection, microarray detection and real-time PCR detection of a variety of nucleic acid targets including microbial species and subspecies, drug resistant mutants, and pathogenic strains.

    摘要翻译： 本发明提供用于使用标记的多核苷酸探针和部分互补的抗体来检测未标记的核酸靶的新型组合物和方法。 探针，对手和靶标的相互作用导致指示目标频率的信号变化。 这种新型检测机制称为DNA检测开关，可实现多种核酸靶点（包括微生物物种和亚种，耐药性突变体和致病菌株）的端点检测，微阵列检测和实时PCR检测。