公开(公告)号：US20050062759A1

公开(公告)日：2005-03-24

申请号：US10496344

申请日：2002-11-28

申请人： Michael Ganser ,  Joachim Wesner

发明人： Michael Ganser ,  Joachim Wesner

IPC分类号： G06T5/00 ,  G09G5/00

CPC分类号： B82Y15/00 ,  G06T5/003 ,  G06T2207/10056

摘要： A method for blind deconvolution of microscopic images in which only a single parameter is varied for the estimation of the PSF. The single parameter takes account of the optical properties of the environment of the object (40) between the objective (20) of the microscope (1) and the region above the object. The single parameter represents a functional relationship among the parameters of the individual layers.

摘要翻译： 一种用于微观图像的盲解卷积方法，其中仅改变单个参数以用于PSF的估计。 单个参数考虑了显微镜（1）的物镜（20）和物体上方的区域（40）之间的物体（40）的环境的光学性质。 单个参数表示各层参数之间的函数关系。

公开(公告)号：US07091990B2

公开(公告)日：2006-08-15

申请号：US10496344

申请日：2002-11-28

申请人： Michael Ganser ,  Joachim Wesner

发明人： Michael Ganser ,  Joachim Wesner

IPC分类号： G06T15/00

CPC分类号： B82Y15/00 ,  G06T5/003 ,  G06T2207/10056

摘要： A method for blind deconvolution of microscopic images in which only a single parameter is varied for the estimation of the PSF. The single parameter takes account of the optical properties of the environment of the object (40) between the objective (20) of the microscope (1) and the region above the object. The single parameter represents a functional relationship among the parameters of the individual layers.

摘要翻译： 一种用于微观图像的盲解卷积方法，其中仅改变单个参数以用于PSF的估计。 单个参数考虑了显微镜（1）的物镜（20）和物体上方的区域（40）之间的物体（40）的环境的光学性质。 单个参数表示各层参数之间的函数关系。

公开(公告)号：US06787301B2

公开(公告)日：2004-09-07

申请号：US09943093

申请日：2001-08-31

申请人： Michael Ganser ,  Albrecht Weiss ,  Joachim Wesner ,  Gerhard Johannsen

发明人： Michael Ganser ,  Albrecht Weiss ,  Joachim Wesner ,  Gerhard Johannsen

IPC分类号： C12Q100

CPC分类号： B23K26/0736 ,  B23K2103/32 ,  B23K2103/50 ,  G01N1/2813 ,  G01N2001/045 ,  G01N2001/2886 ,  Y10S435/808 ,  Y10T83/04

摘要： A method and an apparatus for laser microdissection of specimen regions (23) of interest of a specimen (4) are described. In a first step, an incomplete cut line (25) enclosing the specimen region (23) of interest is generated by means of a laser beam (7). At the incomplete point of the cut line (25), there remains a web (26) which joins the specimen region (23) of interest to the surrounding specimen (4). In a second step, the web (26) is severed with a single laser pulse directed onto it, thereby completing the cut line. The specimen region (23) of interest is in that context detached from the specimen (4) and falls by the action of gravity into a collection vessel (19).

摘要翻译： 描述了用于样本（4）的感兴趣的样本区域（23）的激光显微切割的方法和装置。 在第一步骤中，通过激光束（7）产生包围感兴趣的样本区域（23）的不完整切割线（25）。 在切割线（25）的不完整点处，存在将感兴趣的样品区域（23）连接到周围样品（4）的网（26）。 在第二步骤中，用指向其上的单个激光脉冲切断网（26），从而完成切割线。 感兴趣的样本区域（23）在该情况下与试样（4）分离，并且通过重力作用而落入收集容器（19）中。

公开(公告)号：US08922885B2

公开(公告)日：2014-12-30

申请号：US12032435

申请日：2008-02-15

申请人： Michael Ganser ,  Albrecht Weiss

发明人： Michael Ganser ,  Albrecht Weiss

IPC分类号： G02B21/06 ,  G02B21/16 ,  G02B27/10

CPC分类号： G02B21/16 ,  G02B21/06 ,  G02B27/1006

摘要： An arrangement for fluorescence microscopic examination of specimens includes: a fluorescence microscope; an illumination device that includes: a housing including an interface configured to optically couple the housing and the fluorescence microscope; a plurality of light-emitting diodes disposed in the housing; a respective collector disposed downstream of each of the light emitting diodes and configured to generate a directed light flux; and at least one dichroic splitter disposed in the housing, the at least one splitter and the light-emitting diodes being spatially disposed with respect to one another so that the directed light fluxes are combinable via the at least one splitter into a common illumination beam path directed onto the interface; and a logical control device common to the fluorescence microscope and the illumination device.

摘要翻译： 用于荧光显微镜检查的样品的布置包括：荧光显微镜; 一种照明装置，包括：壳体，包括被配置为光耦合所述壳体和所述荧光显微镜的接口; 设置在所述壳体中的多个发光二极管; 设置在每个所述发光二极管的下游并且被配置为产生有向光通量的相应集电极; 以及设置在所述壳体中的至少一个二向色分配器，所述至少一个分离器和所述发光二极管相对于彼此在空间上设置，使得所述定向光束可经由所述至少一个分离器组合成公共照明光束路径 指向界面; 以及荧光显微镜和照明装置通用的逻辑控制装置。

公开(公告)号：US20090195646A1

公开(公告)日：2009-08-06

申请号：US12300108

申请日：2007-03-28

申请人： Michael Ganser ,  Albrecht Weiss

发明人： Michael Ganser ,  Albrecht Weiss

IPC分类号： H04N7/18 ,  H04N5/225

CPC分类号： G02B21/361

摘要： A microscope comprising a camera connection in a image beam path is suggested. A camera adapter and a camera can be adapted to the camera connection, and the microscope can be used to reproduce the image of a sample on a sensor of the camera. Imaging of the beam path in the camera adapter comprises an intermediate imaging with a pupil plane having reduced beam diameter next to the pupil plane, and at least one optical means can be inserted into the reproduction beam path in the region of the reduced beam cross-section. An optical means or an interchangeable device comprising a plurality of optical means, such as filters, plane-parallel plates or wedge plate, is integrated into the pupil plane in the camera adapter.

摘要翻译： 建议包括图像光束路径中的相机连接的显微镜。 相机适配器和相机可以适应相机连接，并且显微镜可用于在相机的传感器上再现样品的图像。 照相机适配器中的光束路径的成像包括具有瞳孔平面的光瞳面的中间成像，瞳平面的光束直径减小，并且至少一个光学装置可以在缩小光束横截面的区域插入到再现光束路径中， 部分。 包括多个光学装置（诸如滤光器，平面平行板或楔形板）的光学装置或可互换装置被集成到相机适配器中的光瞳平面中。

公开(公告)号：US06545765B1

公开(公告)日：2003-04-08

申请号：US09708778

申请日：2000-11-08

申请人： Michael Ganser ,  Albrecht Weiss

发明人： Michael Ganser ,  Albrecht Weiss

IPC分类号： G01B1106

CPC分类号： G01B11/0616

摘要： A method and an apparatus for measuring thickness of transparent films is described in which an illumination beam is directed through an objective onto an object comprising a transparent film. A structured focusing aid is disposed in the illumination beam, a camera is disposed in an imaging beam. The focusing aid and the camera each are disposed in locations conjugated with the focal plane of the objective. The focal plane of the objective is displaced stepwise through the object. At each position, a camera image is recorded and its focus score is determined, the image of the focusing aid being used as the contrast indicator. The positions with maximal focus scores are assigned to the locations of the interfaces. The thickness of the transparent film is calculated from the difference between the positions of its interfaces.

摘要翻译： 描述了一种用于测量透明膜的厚度的方法和装置，其中将照明光束通过物镜引导到包括透明膜的物体上。 结构化聚焦辅助设置在照明光束中，照相机设置在成像光束中。 聚焦辅助器和照相机各自设置在与物镜的焦平面共轭的位置。 物镜的焦平面通过物体逐步移位。 在每个位置，记录摄像机图像并确定其对焦分数，聚焦辅助图像被用作对比度指示器。 具有最大聚焦分数的位置被分配给接口的位置。 透明膜的厚度根据其界面的位置之间的差异来计算。

公开(公告)号：US06369939B1

公开(公告)日：2002-04-09

申请号：US09555844

申请日：2000-06-05

申请人： Albrecht Weiss ,  Michael Ganser ,  Helmut Rühl ,  Manfred Gilbert

发明人： Albrecht Weiss ,  Michael Ganser ,  Helmut Rühl ,  Manfred Gilbert

IPC分类号： G02B2112

CPC分类号： G02B21/086

摘要： An illumination system (1) for a microscope, in which a first light source (2), a collector lens (3), an aperture diaphragm (4), and a condenser lens ((5) are arranged in the illumination beam path (6), is described. The illumination light is directed through the aperture diaphragm (4) via the condenser lens (5) into the object plane (9). Arranged in the illumination beam path (6) on the optical axis (20) is a second light source (8) that is imaged at infinity by the condenser lens (5).

摘要翻译： 一种用于显微镜的照明系统（1），其中第一光源（2），集光透镜（3），孔径光阑（4）和聚光透镜（（5））布置在照明光束路径 照明光通过聚光透镜（5）通过孔径光阑（4）引导到物平面（9）中，在光轴（20）上的照明光束路径（6）中排列为 由聚光透镜（5）在无限远处成像的第二光源（8）。

公开(公告)号：US20130027770A1

公开(公告)日：2013-01-31

申请号：US13559778

申请日：2012-07-27

申请人： Michael Ganser ,  Albrecht Weiss

发明人： Michael Ganser ,  Albrecht Weiss

IPC分类号： G02B21/06

CPC分类号： G02B21/088 ,  G02B5/22 ,  G02B21/125 ,  G02B21/16

摘要： The invention relates to a microscope illumination method, to a microscope illumination set, comprising a white light LED (4) and a correction filter (6), and to a corresponding microscope system (1) for analysing a sample (10) alternately or simultaneously in transmitted light bright field illumination and in incident light fluorescence illumination, wherein a white light LED (4) is used for the transmitted light bright field illumination, and a correction filter (6) is activated at a location in the illumination beam path of the transmitted light bright field illumination both during transmitted light bright field illumination and during incident light fluorescence illumination, wherein the correction filter (6) has a spectral transmission profile which has a minimum in the wavelength range of at least one maximum of the spectrum of the white light LED (4).

摘要翻译： 本发明涉及一种显微照明方法，涉及一种包括白光LED（4）和校正滤光片（6）的显微照明组件，以及用于交替或同时分析样品（10）的相应显微系统（1） 在透射光明场照明和入射光荧光照明中，其中白光LED（4）用于透射光明场照明，校正滤光片（6）在照明光束路径中的位置被激活 在透射光明场照明期间和在入射光荧光照明期间的透射光明场照明，其中所述校正滤光片（6）具有光谱透射分布，所述光谱透射分布在白色光谱的至少一个最大值的波长范围内具有最小值 灯LED（4）。

公开(公告)号：US20080198448A1

公开(公告)日：2008-08-21

申请号：US12032435

申请日：2008-02-15

申请人： Michael Ganser ,  Albrecht Weiss

发明人： Michael Ganser ,  Albrecht Weiss

IPC分类号： G02B21/06

CPC分类号： G02B21/16 ,  G02B21/06 ,  G02B27/1006

摘要： An arrangement for fluorescence microscopic examination of specimens includes: a fluorescence microscope; an illumination device that includes: a housing including an interface configured to optically couple the housing and the fluorescence microscope; a plurality of light-emitting diodes disposed in the housing; a respective collector disposed downstream of each of the light emitting diodes and configured to generate a directed light flux; and at least one dichroic splitter disposed in the housing, the at least one splitter and the light-emitting diodes being spatially disposed with respect to one another so that the directed light fluxes are combinable via the at least one splitter into a common illumination beam path directed onto the interface; and a logical control device common to the fluorescence microscope and the illumination device.

摘要翻译： 用于荧光显微镜检查的样品的布置包括：荧光显微镜; 一种照明装置，包括：壳体，包括被配置为光耦合所述壳体和所述荧光显微镜的接口; 设置在所述壳体中的多个发光二极管; 设置在每个所述发光二极管的下游并且被配置为产生有向光通量的相应集电极; 以及设置在所述壳体中的至少一个二向色分配器，所述至少一个分离器和所述发光二极管相对于彼此在空间上设置，使得所述定向光束可经由所述至少一个分离器组合成公共照明光束路径 指向界面; 以及荧光显微镜和照明装置通用的逻辑控制装置。

公开(公告)号：US20060291040A1

公开(公告)日：2006-12-28

申请号：US11421127

申请日：2006-05-31

申请人： Michael Ganser

发明人： Michael Ganser

IPC分类号： G02B21/00

CPC分类号： G02B21/36 ,  G02B21/365

摘要： A microscope system and an image production method utilizing a microscope having one or more setting elements for modifying at least one microscope setting, and a camera for acquiring a microscope image, which camera is operable in predeterminable image modes. At least one of the setting elements of the microscope is operatively connected to the camera in such a way that a specific image mode of the camera is settable as a function of a modification of the microscope setting. Alternatively or additionally, a microscope setting can be modified as a function of an image mode of the camera that is present.

摘要翻译： 显微镜系统和使用具有用于修改至少一个显微镜设置的一个或多个设置元件的显微镜的显微镜系统和图像产生方法以及用于获取显微镜图像的相机，该相机可在可预定的图像模式中操作。 显微镜的至少一个设置元件可操作地连接到相机，使得相机的特定图像模式可以根据显微镜设置的修改来设置。 或者或另外，可以根据存在的相机的图像模式来修改显微镜设置。