公开(公告)号：US10107797B2

公开(公告)日：2018-10-23

申请号：US14830539

申请日：2015-08-19

申请人： Micronics, Inc.

发明人： C. Frederick Battrell ,  Diane Wierzbicki ,  John Clemmens ,  Jason Capodanno ,  John R. Williford ,  Carolina Elmufdi ,  Isaac Sprague

IPC分类号： B01L3/00 ,  G01N33/53 ,  G01N33/80

摘要： Microfluidic cartridges for agglutination reactions are provided. The cartridges include a microfluidic reaction channel with at least two intake channels, one for an antigen-containing fluid and the other for an antibody-containing fluid, conjoined to a reaction channel modified by incorporation of a downstream flow control channel. At low Reynolds Number, the two input streams layer one on top of the other in the reaction channel and form a flowing, unmixed horizontally-stratified laminar fluid diffusion (HLFD) interface for an extended duration of reaction. Surprisingly, the design, surface properties, and flow regime of microfluidic circuits of the present invention potentiate detection of antibody mediated agglutination at the stratified interface. Antigen:antibody reactions involving agglutination potentiated by these devices are useful in blood typing, in crossmatching for blood transfusion, and in immunodiagnostic agglutination assays, for example.

公开(公告)号：US20160209431A1

公开(公告)日：2016-07-21

申请号：US14830539

申请日：2015-08-19

申请人： Micronics, Inc.

发明人： C. Frederick Battrell ,  Diane Wierzbicki ,  John Clemmens ,  Jason Capodanno ,  John R. Williford ,  Carolina Elmufdi ,  Isaac Sprague

IPC分类号： G01N33/80 ,  B01L3/00

CPC分类号： G01N33/53 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/161 ,  G01N33/5304 ,  G01N33/80

摘要： Microfluidic cartridges for agglutination reactions are provided. The cartridges include a microfluidic reaction channel with at least two intake channels, one for an antigen-containing fluid and the other for an antibody-containing fluid, conjoined to a reaction channel modified by incorporation of a downstream flow control channel. At low Reynolds Number, the two input streams layer one on top of the other in the reaction channel and form a flowing, unmixed horizontally-stratified laminar fluid diffusion (HLFD) interface for an extended duration of reaction. Surprisingly, the design, surface properties, and flow regime of microfluidic circuits of the present invention potentiate detection of antibody mediated agglutination at the stratified interface. Antigen:antibody reactions involving agglutination potentiated by these devices are useful in blood typing, in crossmatching for blood transfusion, and in immunodiagnostic agglutination assays, for example.

公开(公告)号：US20130142708A1

公开(公告)日：2013-06-06

申请号：US13660834

申请日：2012-10-25

申请人： Micronics, Inc.

发明人： C. Frederick Battrell ,  Diane Wierzbicki ,  John Clemmens ,  Jason Capodanno ,  John R. Williford ,  Carolina Elmufdi ,  Isaac Sprague

IPC分类号： G01N33/80

CPC分类号： G01N33/53 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/161 ,  G01N33/5304 ,  G01N33/80

摘要： Microfluidic cartridges for agglutination reactions are provided. The cartridges include a microfluidic reaction channel with at least two intake channels, one for an antigen-containing fluid and the other for an antibody-containing fluid, conjoined to a reaction channel modified by incorporation of a downstream flow control channel. At low Reynolds Number, the two input streams layer one on top of the other in the reaction channel and form a flowing, unmixed horizontally-stratified laminar fluid diffusion (HLFD) interface for an extended duration of reaction. Surprisingly, the design, surface properties, and flow regime of microfluidic circuits of the present invention potentiate detection of antibody mediated agglutination at the stratified interface. Antigen:antibody reactions involving agglutination potentiated by these devices are useful in blood typing, in crossmatching for blood transfusion, and in immunodiagnostic agglutination assays, for example.

摘要翻译： 提供用于凝集反应的微流控盒。 药筒包括具有至少两个进气通道的微流体反应通道，一个用于含抗原的流体，另一个用于含抗体流体，结合到通过并入下游流动控制通道而修改的反应通道。 在低雷诺数下，两个输入流在反应通道中层叠在另一个之上，并形成流动的，未混合的水平分层的层状流体扩散（HLFD）界面，持续延长的反应时间。 令人惊奇的是，本发明的微流体回路的设计，表面性质和流动状态增强了在分层界面处的抗体介导的凝集的检测。 抗原：涉及通过这些装置增强的凝集的抗体反应可用于血型，输血交配和免疫诊断凝集试验。

公开(公告)号：US09146246B2

公开(公告)日：2015-09-29

申请号：US13660834

申请日：2012-10-25

申请人： Micronics, Inc.

发明人： C. Frederick Battrell ,  Diane Wierzbicki ,  John Clemmens ,  Jason Capodanno ,  John R. Williford ,  Carolina Elmufdi ,  Isaac Sprague

IPC分类号： B01L3/00 ,  G01N33/80 ,  G01N33/53

CPC分类号： G01N33/53 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/161 ,  G01N33/5304 ,  G01N33/80

摘要： Microfluidic cartridges for agglutination reactions are provided. The cartridges include a microfluidic reaction channel with at least two intake channels, one for an antigen-containing fluid and the other for an antibody-containing fluid, conjoined to a reaction channel modified by incorporation of a downstream flow control channel. At low Reynolds Number, the two input streams layer one on top of the other in the reaction channel and form a flowing, unmixed horizontally-stratified laminar fluid diffusion (HLFD) interface for an extended duration of reaction. Surprisingly, the design, surface properties, and flow regime of microfluidic circuits of the present invention potentiate detection of antibody mediated agglutination at the stratified interface. Antigen:antibody reactions involving agglutination potentiated by these devices are useful in blood typing, in crossmatching for blood transfusion, and in immunodiagnostic agglutination assays, for example.

摘要翻译： 提供用于凝集反应的微流控盒。 药筒包括具有至少两个进气通道的微流体反应通道，一个用于含抗原的流体，另一个用于含抗体流体，结合到通过并入下游流动控制通道而修改的反应通道。 在低雷诺数下，两个输入流在反应通道中层叠在另一个之上，并形成流动的，未混合的水平分层的层状流体扩散（HLFD）界面，持续延长的反应时间。 令人惊奇的是，本发明的微流体回路的设计，表面性质和流动状态增强了在分层界面处的抗体介导的凝集的检测。 抗原：涉及通过这些装置增强的凝集的抗体反应可用于血型，输血交配和免疫诊断凝集试验。