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    Microfluidic devices and methods for performing serum separation and blood cross-matching
    3.
    发明授权
    Microfluidic devices and methods for performing serum separation and blood cross-matching 有权

    公开(公告)号：US10386377B2

    公开(公告)日：2019-08-20

    申请号：US14889366

    申请日：2014-05-07

    申请人： Micronics, Inc.

    发明人： Andrew W. Kolb ,  Carolina L. Elmufdi ,  C. Frederick Battrell

    IPC分类号： B01L3/00 ,  G01N33/80 ,  G01N33/49

    摘要： Microfluidic cartridges or devices for serum separation and blood cross-match analysis are provided. The devices may include a serum separation subcircuit alone or in combination with a solute mixing subcircuit. The serum separation subcircuit promotes on-cartridge clotting of a blood sample and manipulates the flow of the separated serum sample for subsequent cross-match analysis with a second blood sample, for example. The solute mixing subcircuit includes at least two intake channels, one for a whole blood sample from, for example, a blood donor and the other for the separated serum sample from, for example, a transfusion recipient. The solute mixing subcircuit further includes a serpentine mixing channel conjoined to a downstream channel. Under vacuum generated by a conjoined finger pump, the two input streams fill the serpentine mixing and downstream channels due to capillary action, enabling visualization of an agglutination reaction.

    Microfluidic apparatus and methods for performing blood typing and crossmatching
    4.
    发明授权
    Microfluidic apparatus and methods for performing blood typing and crossmatching 有权

    公开(公告)号：US10107797B2

    公开(公告)日：2018-10-23

    申请号：US14830539

    申请日：2015-08-19

    申请人： Micronics, Inc.

    发明人： C. Frederick Battrell ,  Diane Wierzbicki ,  John Clemmens ,  Jason Capodanno ,  John R. Williford ,  Carolina Elmufdi ,  Isaac Sprague

    IPC分类号： B01L3/00 ,  G01N33/53 ,  G01N33/80

    摘要： Microfluidic cartridges for agglutination reactions are provided. The cartridges include a microfluidic reaction channel with at least two intake channels, one for an antigen-containing fluid and the other for an antibody-containing fluid, conjoined to a reaction channel modified by incorporation of a downstream flow control channel. At low Reynolds Number, the two input streams layer one on top of the other in the reaction channel and form a flowing, unmixed horizontally-stratified laminar fluid diffusion (HLFD) interface for an extended duration of reaction. Surprisingly, the design, surface properties, and flow regime of microfluidic circuits of the present invention potentiate detection of antibody mediated agglutination at the stratified interface. Antigen:antibody reactions involving agglutination potentiated by these devices are useful in blood typing, in crossmatching for blood transfusion, and in immunodiagnostic agglutination assays, for example.

    PORTABLE FLUORESCENCE DETECTION SYSTEM AND MICROASSAY CARTRIDGE
    5.
    发明申请
    PORTABLE FLUORESCENCE DETECTION SYSTEM AND MICROASSAY CARTRIDGE 审中-公开

    公开(公告)号：US20180292319A1

    公开(公告)日：2018-10-11

    申请号：US15804869

    申请日：2017-11-06

    申请人： Micronics, Inc.

    发明人： C. Frederick Battrell ,  Troy D. Daiber ,  William Samuel Hunter

    IPC分类号： G01N21/64 ,  B01L7/00 ,  B01L3/00

    CPC分类号： G01N21/6428 ,  B01L3/502715 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2300/0816 ,  B01L2300/168 ,  B01L2300/1827 ,  B01L2300/1844 ,  B01L2400/0487 ,  G01N21/645 ,  G01N2201/062 ,  G01N2201/12 ,  Y02A90/26

    摘要： Disclosed is a compact, microprocessor-controlled instrument for fluorometric assays in liquid samples, the instrument having a floating stage with docking bay for receiving a microfluidic cartridge and a scanning detector head with on-board embedded microprocessor operated under control of a ODAP daemon resident in the detector head for controlling source LEDs, emission signal amplification and filtering in an isolated, low noise, high-gain environment within the detector head. Multiple optical channels may be incorporated in the scanning head. In a preferred configuration, the assay is validated using dual channel optics for monitoring a first fluorophore associated with a target analyte and a second fluorophore associated with a control. Applications include molecular biological assays based on PCR amplification of target nucleic acids and fluorometric assays in general, many of which require temperature control during detection. Sensitivity and resistance to bubble interference during scanning are shown to be improved by use of a heating block with reflective mirror face in intimate contact with a thermo-optical window enclosing the liquid sample.

    DEVICE FOR PREPARATION AND ANALYSIS OF NUCLEIC ACIDS
    6.
    发明申请
    DEVICE FOR PREPARATION AND ANALYSIS OF NUCLEIC ACIDS 审中-公开
    核酸的制备和分析装置

    公开(公告)号：US20160090588A1

    公开(公告)日：2016-03-31

    申请号：US14889374

    申请日：2014-05-07

    申请人： MICRONICS, INC.

    发明人： Alan K. Lofquist ,  C. Frederick Battrell ,  Heather K. Bouzek

    IPC分类号： C12N15/10 ,  C12Q1/68

    CPC分类号： C12N15/1017 ,  B01L3/502753 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0406 ,  B01L2400/0481 ,  B01L2400/049 ,  C12Q1/6806

    摘要： An integrated “lab-on-a-chip” microfluidic device performs nucleic acid sample preparation and diagnostic analysis from test samples containing cells and/or particles. The device analyzes DNA or RNA targets, or both, from a common test sample. Dried and/or liquid reagents necessary for nucleic acid sample preparation and analysis are contained on the device, such that the device only requires addition of test sample. Clay mineral and alkaline buffer reagents are employed for overcoming the problems of nucleic acid degradation and contamination during sample preparation. The device may include a composite filter to separate plasma or serum from other blood constituents when the test sample is a blood product. The microfluidic device utilizes a plurality of microfluidic channels, inlets, valves, membranes, pumps, and other elements arranged in various configurations to manipulate the flow of the liquid sample, in particular, in order to prepare nucleic acids and perform further diagnostic analysis.

    摘要翻译： 集成的“芯片上实验室”微流体装置从含有细胞和/或颗粒的测试样品进行核酸样品制备和诊断分析。 该设备从普通测试样品中分析DNA或RNA靶标，或两者。 在设备上包含用于核酸样品制备和分析所需的干燥和/或液体试剂，使得该装置仅需要加入测试样品。 粘土矿物和碱性缓冲剂用于克服样品制备过程中核酸降解和污染的问题。 当测试样品是血液制品时，该装置可以包括复合过滤器以将血浆或血清与其它血液成分分离。 微流体装置利用多个微流体通道，入口，阀，膜，泵和以各种配置布置的其它元件来操纵液体样品的流动，特别是为了制备核酸并进行进一步的诊断分析。

    Lapping machine and method
    9.
    发明授权
    Lapping machine and method 失效
    LAPPING MACHINE AND METHOD

    公开(公告)号：US3653160A

    公开(公告)日：1972-04-04

    申请号：US3653160D

    申请日：1969-04-23

    申请人： IND MICRONICS INC

    发明人： RAICKLE JOHN A

    IPC分类号： B24B37/08 ,  B24B7/02

    CPC分类号： B24B37/08

    摘要： A lapping machine employing first and second lapping discs mounted coaxially for rotation and having first and second air cushion means for biassing the discs outwardly with respect to each other against the inwardly biassing force of spring means engaging the outer sides of the discs with the air cushion means being mounted for axial adjustment with respect to each other to consequently adjust the axial distance between the discs and with such adjustments being enabled by means of adjustable shift plates supporting the air cushion means and movable toward and from each other by means of first and second dovetail wedge block couples located on each end of said plates so that relative movement of the plates with respect to each other causes the plates to move toward or away from each other.

    摘要翻译： 一种研磨机，其使用同轴地旋转的第一和第二研磨盘，并具有第一和第二气垫装置，用于相对于彼此向外偏压所述盘，以克服弹簧装置的向内偏移力，所述弹簧装置与盘的外侧接合， 装置被安装用于相对于彼此轴向调节，从而调节盘之间的轴向距离，并且借助于可调整的移位板，借助于可调节的移动板来支撑气垫装置，并通过第一和第二 位于所述板的每个端部上的燕尾楔块组合使得板相对于彼此的相对运动导致板彼此移动或远离。

    Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions
    10.
    发明授权
    Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions 有权

    公开(公告)号：US10190153B2

    公开(公告)日：2019-01-29

    申请号：US14889370

    申请日：2014-05-07

    申请人： Micronics, Inc.

    发明人： Heather K. Bouzek

    IPC分类号： C12Q1/68 ,  C12Q1/6806 ,  C12N15/10 ,  C12Q1/70 ,  C07H21/02

    摘要： The present invention provides a rapid and highly effective method for the preparation of biological samples for detection of both DNA and RNA target molecules. The method comprises treatment of the sample with a clay mineral followed by lysis of the sample with an alkaline solution. The method is particularly suited for preparing complex biological samples, such as blood or plasma, for the simultaneous detection of DNA and RNA targets. Samples prepared by the method of the invention may be directly used as targets in PCR amplification. The method of the invention may conveniently be coupled with further steps and devices to perform molecular analyses for diagnostic and other applications.