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公开(公告)号：US12121898B2
公开(公告)日：2024-10-22
申请号：US17148287
申请日：2021-01-13
发明人： David A. Weitz , Adam R. Abate
IPC分类号： B01L3/00 , B01F23/41 , B01F33/3011 , B01F33/302 , B01L3/02
CPC分类号： B01L3/502761 , B01F23/41 , B01F33/3011 , B01F33/3021 , B01L3/0241 , B01L3/502784 , B01L2200/0636 , B01L2200/0652 , B01L2300/0681 , Y10T137/0318 , Y10T137/8593
摘要： The present invention is generally related to systems and methods for producing droplets. The droplets may contain varying species, e.g., for use as a library. In some cases, at least one droplet is used to create a plurality of droplets, using techniques such as flow-focusing techniques. In one set of embodiments, a plurality of droplets, containing varying species, can be divided to form a collection of droplets containing the various species therein. A collection of droplets, according to certain embodiments, may contain various subpopulations of droplets that all contain the same species therein. Such a collection of droplets may be used as a library in some cases, or may be used for other purposes.
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2.发明公开公开(公告)号：US20240316557A1
公开(公告)日：2024-09-26
申请号：US18607538
申请日：2024-03-17
发明人： Jingxuan TIAN , Ho Cheung SHUM , Aditi DEY POONAM , Lang NAN
CPC分类号： B01L3/502784 , B01L7/525 , B01L2200/0636 , B01L2200/0673 , B01L2200/147 , B01L2300/0883 , B01L2300/1822
摘要： A portable, miniaturized microfluidic droplet-based digital polymerase chain reaction (PCR) device is provided. The device includes a droplet generation layer with a flow-focusing channel and multiple capillary channels, in which the flow-focusing channel has a flow focusing structure to generate droplets from a provided aqueous sample and extract oil, and the multiple capillary channels is configured to perform an oil extraction. An incubation layer includes heating plates, a microfluidic channel, and a container for conducting a PCR thermal cycling program. Controlled by a controller and powered by a power supplier, the PCR thermal cycling program involves maintaining predefined temperatures on each heating plate sequentially. This process ensures the heating of droplets to different temperatures at distinct times, facilitating precise and efficient PCR amplification.
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3.发明授权公开(公告)号：US12083521B2
公开(公告)日：2024-09-10
申请号：US16626647
申请日：2018-07-02
申请人： BLINK AG
IPC分类号： B01F35/513 , B01F23/411 , B01F31/20 , B01L3/00 , B01L7/00 , B01L99/00 , C12M1/00 , C12M1/34 , G01N15/00 , G01N15/1433 , G01N15/14
CPC分类号： B01L7/52 , B01F23/4111 , B01F31/20 , B01F35/513 , B01L3/502776 , B01L3/505 , C12M41/14 , G01N15/1433 , B01L2200/0636 , B01L2200/0684 , B01L2300/042 , B01L2300/0681 , B01L2300/0832 , B01L2300/1822 , B01L2400/0409 , B01L2400/0457 , B01L2400/0481 , B01L2400/049 , B01L2400/0655 , G01N2015/1493
摘要： The present invention relates to a sample cartridge for incubating and/or analyzing a dispersion of particles, cells or droplets and/or for performing biochemical reactions with or in such dispersion. The present invention furthermore relates to a device for incubating a dispersion of particles, cells or droplets and/or for performing a biochemical reaction therewith. Moreover, the present invention also relates to the use of a sample cartridge or of a device for generating and/or processing a dispersion of particles, cells or droplets. Moreover, the present invention relates to a method of processing a dispersion of particles, cells or droplets. Furthermore, the present invention relates to a method of generating a dispersion of droplets and to a method of generating a dispersion of solid or semi-solid particles.
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公开(公告)号：US20240210301A1
公开(公告)日：2024-06-27
申请号：US18288218
申请日：2022-04-22
申请人： ETH Zurich
发明人： Andrew De Mello , Stavros Stavrakis , Xiaobao Cao , Mohammad Asghari , Mahmut Aslan , Bogdan Mateescu , Yingchao Meng
IPC分类号： G01N15/1404 , B01L3/00 , G01N15/14 , G01N15/1433
CPC分类号： G01N15/1404 , B01L3/502761 , G01N15/1433 , G01N15/1484 , B01L2200/0636 , G01N2015/1495
摘要： In a method of investigating cell deformations, a sample fluid including cells suspended in a suspending medium is provided. A flow of the sample fluid through a focusing microchannel is established. The suspending medium is a non-Newtonian fluid having viscoelastic properties such that cells that enter the focusing microchannel are focused towards a center of the focusing microchannel due to the viscoelastic properties of the suspending medium, causing the cells to exit the focusing microchannel in single file. Subsequently, the sample fluid that has exited the focusing microchannel is caused to flow through a deformation microchannel arranged downstream of the focusing microchannel to cause a deformation of cells that have exited the focusing microchannel and have entered the deformation microchannel, the deformation being caused by a flow pattern created by interaction of the fluid flow with the deformation microchannel.
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公开(公告)号：US20240141421A1
公开(公告)日：2024-05-02
申请号：US18384971
申请日：2023-10-30
申请人： Stokes Bio Limited
发明人： Mark DAVIES , Tara DALTON
CPC分类号： C12Q1/686 , B01F25/10 , B01F33/30 , B01L3/50273 , B01L3/502776 , B01L3/502784 , B01L7/525 , G01N35/08 , B01L2200/0636 , B01L2200/0673 , B01L2300/087 , B01L2400/0409 , B01L2400/0487 , G01N35/1095
摘要： A microfluidic analysis system (1) performs polymerase chain reaction (PCR) analysis on a bio sample. In a centrifuge (6) the sample is separated into DNA and RNA constituents. The vortex is created by opposing flow of a silicon oil primary carrier fluid effecting circulation by viscous drag. The bio sample exits the centrifuge enveloped in the primary carrier fluid. This is pumped by a flow controller (7) to a thermal stage (9). The thermal stage (9) has a number of microfluidic devices (70) each having thermal zones (71, 72, 73) in which the bio sample is heated or cooled by heat conduction to/from a thermal carrier fluid and the primary carrier fluid. Thus, the carrier fluids envelope the sample, control its flowrate, and control its temperature without need for moving parts at the micro scale.
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公开(公告)号：US11964244B2
公开(公告)日：2024-04-23
申请号：US17197177
申请日：2021-03-10
申请人： STOKES BIO LIMITED
发明人： Mark Davies , Tara Dalton
IPC分类号： B01L3/00 , B01F25/10 , B01F33/30 , B01F33/302 , B01L7/00 , G01N33/50 , G01N35/08 , G01N35/00 , G01N35/10
CPC分类号： B01F25/10 , B01F33/30 , B01F33/3021 , B01L3/502784 , B01L7/525 , G01N33/5008 , G01N35/08 , B01L2200/0636 , B01L2200/0673 , B01L2300/0816 , B01L2300/0861 , B01L2300/087 , B01L2300/185 , B01L2400/0409 , B01L2400/0487 , G01N2035/00514 , G01N35/1095
摘要： A system for analyzing a biological sample may include at least one sample acquisition stage comprising a sample acquisition device for acquiring the biological sample from a sample source; a droplet generator device for forming a droplet wrapped in an immiscible carrier fluid, wherein the wrapped droplet comprises at least the biological sample and a reagent, the droplet generator configured to receive the biological sample transferred from the sample acquisition device; a collection vessel for collecting the wrapped sample droplet from the droplet generator, the vessel configured to contain a carrier fluid for receiving and protecting the sample droplet; and an analysis system for analyzing the wrapped sample droplet and detecting products of a polymerase chain reaction.
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7.发明授权公开(公告)号：US11890616B2
公开(公告)日：2024-02-06
申请号：US16830617
申请日：2020-03-26
发明人： Jae Wan Kwon , Jussuf Kaifi
CPC分类号： B01L3/502761 , G01N15/1056 , B01L2200/0636 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2300/0861 , B01L2300/0864 , B01L2400/086
摘要： The subject matter described herein relates to devices and methods for capturing micrometer scale objects from a fluid flow, and more particularly, to devices and methods for capturing cells. The devices generally comprise an array of posts configured and arranged to selectively direct the micrometer scale objects toward trapping channels defined between posts in which the micrometer scale objects can be trapped, and to direct smaller particles through bypass channels around the posts. Methods for using the devices to trap cells, analyze cells and treat cancer are also described.
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公开(公告)号：US11873173B2
公开(公告)日：2024-01-16
申请号：US18136126
申请日：2023-04-18
申请人： CYTONOME/ST, LLC
IPC分类号： B65G51/08 , B01L3/00 , G01N15/14 , B07C5/00 , B07C5/34 , B01F33/301 , B65G51/00 , B01F33/3011
CPC分类号： B65G51/08 , B01F33/3017 , B01L3/502776 , B07C5/00 , B07C5/34 , G01N15/1404 , G01N15/1484 , B01F33/3011 , B01J2219/0086 , B01J2219/00822 , B01J2219/00831 , B01J2219/00833 , B01J2219/00891 , B01L2200/0636 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0887 , B01L2400/0487 , B65G51/00 , G01N2015/1409
摘要： A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.
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公开(公告)号：US20230405578A1
公开(公告)日：2023-12-21
申请号：US18032756
申请日：2021-09-28
申请人： QuantaMatrix Inc.
发明人： Jung Il CHOI , Sun Jae HWANG
IPC分类号： B01L3/00
CPC分类号： B01L3/502 , B01L2200/0636 , B01L2300/087 , B01L2300/0663 , B01L2300/0803 , B01L2400/0409
摘要： An antimicrobial susceptibility testing device according to an embodiment of the present invention includes a substrate and a driving unit rotating the substrate. The substrate includes first injection holes formed so as to penetrate the upper surface thereof and channels forming passages therein through which antibiotics, a bacteria sample or mixed solutions thereof injected through the first injection holes are movable and having first regions where the mixed solutions are concentrated and localized by centrifugal force applied when the substrate is rotated by the driving unit.
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公开(公告)号：US20230381781A1
公开(公告)日：2023-11-30
申请号：US18318294
申请日：2023-05-16
申请人： Intabio, LLC
发明人： Luc BOUSSE , Don Wesley ARNOLD , Wesley CHANG , Erik GENTALEN , Eric GWERDER , Scott MACK
IPC分类号： B01L3/00 , G01N27/447
CPC分类号： B01L3/502776 , B01L2400/0427 , B01L3/502761 , G01N27/44795 , B01L3/5027 , B01L3/502715 , G01N27/44791 , B01L2200/0647 , B01L2300/0645 , B01L2300/161 , B01L2300/12 , B01L2200/0636 , B01L2300/047 , B01L2300/0816 , B01L2300/0864 , B01L2200/0684 , B01L2400/0421 , B01L3/502792
摘要： Methods, devices, and systems for performing isoelectric focusing reactions are described. The systems or devices disclosed herein may comprise fixtures that have a membrane. In some instances, the disclosed devices may be designed to perform isoelectric focusing or other separation reactions followed by further characterization of the separated analytes using mass spectrometry. Two or more isoelectric focusing reactions may be performed in parallel. The disclosed methods, devices, and systems provide for fast, accurate separation and characterization of protein analyte mixtures or other biological molecules by isoelectric point.
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