公开(公告)号：US5879686A

公开(公告)日：1999-03-09

申请号：US853504

申请日：1997-05-08

申请人： Milan S. Blake ,  Joseph Y. Tai ,  Huilin L. Qi ,  Shu-Mei Liang ,  Lucjan J. J. Hronowski ,  Jeffrey K. Pullen

发明人： Milan S. Blake ,  Joseph Y. Tai ,  Huilin L. Qi ,  Shu-Mei Liang ,  Lucjan J. J. Hronowski ,  Jeffrey K. Pullen

IPC分类号： C12N15/09 ,  A61K39/00 ,  A61K39/095 ,  A61P31/04 ,  C07K1/18 ,  C07K14/01 ,  C07K14/22 ,  C12N1/21 ,  C12N15/31 ,  C12N15/70 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  C12R1/36

CPC分类号： C07K14/005 ,  C07K14/22 ,  C12N15/70 ,  A61K39/00 ,  C07K2319/00 ,  C12N2795/10022 ,  Y10S435/849

摘要： The present invention relates, in general, to a method for the high level expression of the outer membrane protein meningococcal group B porin proteins and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein meningococcal group B porin proteins in E. coli wherein the meningococcal group B porin proteins and fusion proteins thereof comprise more than 2% of the total protein expressed in E. coli. The invention also relates to a method of purification and refolding of the meningococcal group B porin proteins and fusion proteins thereof and to their use in vaccines.

公开(公告)号：US5747287A

公开(公告)日：1998-05-05

申请号：US877109

申请日：1997-06-17

申请人： Milan S. Blake ,  Joseph Y. Tai ,  Huilin L. Qi ,  Shu-Mei Liang ,  Lucjan J. J. Hronowski ,  Jeffrey K. Pullen

发明人： Milan S. Blake ,  Joseph Y. Tai ,  Huilin L. Qi ,  Shu-Mei Liang ,  Lucjan J. J. Hronowski ,  Jeffrey K. Pullen

IPC分类号： A61K39/00 ,  C07K14/01 ,  C07K14/22 ,  C07K1/00 ,  A61K39/05 ,  C12N15/00 ,  C12P21/06

CPC分类号： C07K14/005 ,  C07K14/22 ,  A61K39/00 ,  C07K2319/00 ,  C12N2795/10022

摘要： The present invention relates, in general, to a method for the high level expression of the outer membrane protein meningococcal group B porin proteins and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein meningococcal group B porin proteins in E. coli wherein the meningococcal group B porin proteins and fusion proteins thereof comprise more than 2% of the total protein expressed in E. coli. The invention also relates to a method of purification and refolding of the meningococcal group B porin proteins and fusion proteins thereof and to their use in vaccines.

公开(公告)号：US5439808A

公开(公告)日：1995-08-08

申请号：US96182

申请日：1993-07-23

申请人： Milan S. Blake ,  Joseph Y. Tai ,  Huilin L. Qi ,  Shu-Mei Liang ,  Lucjan J. J. Hronowski ,  Jeffrey K. Pullen

发明人： Milan S. Blake ,  Joseph Y. Tai ,  Huilin L. Qi ,  Shu-Mei Liang ,  Lucjan J. J. Hronowski ,  Jeffrey K. Pullen

IPC分类号： C12N15/09 ,  A61K39/00 ,  A61K39/095 ,  A61P31/04 ,  C07K1/18 ,  C07K14/01 ,  C07K14/22 ,  C12N1/21 ,  C12N15/31 ,  C12N15/70 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  C12R1/36 ,  C12N15/62 ,  C12N15/67

CPC分类号： C07K14/005 ,  C07K14/22 ,  C12N15/70 ,  A61K39/00 ,  C07K2319/00 ,  C12N2795/10022 ,  Y10S435/849

摘要： The present invention relates, in general, to a method for the high level expression of the outer membrane protein meningococcal group B porin proteins and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein meningococcal group B porin proteins in E. coli wherein the meningococcal group B porin proteins yard fusion proteins thereof comprise more than 2% of the total protein expressed in E. coli. The invention also relates to a method of purification and refolding of the meningococcal group B porin proteins and fusion proteins thereof and to their use in vaccines.

摘要翻译： 本发明一般涉及外膜蛋白脑膜炎球菌B族孔蛋白及其融合蛋白的高水平表达的方法。 特别地，本发明涉及在大肠杆菌中表达外膜蛋白质脑膜炎双球菌B蛋白质的蛋白质的方法，其中脑膜炎球菌B蛋白质组蛋白质的蛋白质融合蛋白质包含在大肠杆菌中表达的总蛋白的2％以上 。 本发明还涉及脑膜炎球菌B族孔蛋白及其融合蛋白的纯化和重折叠方法及其在疫苗中的应用。

公开(公告)号：US6013267A

公开(公告)日：2000-01-11

申请号：US798760

申请日：1997-02-11

申请人： Milan S. Blake ,  Joseph Y. Tai ,  Huilin L. Qi ,  Shu-Mei Liang ,  Lucjan J.J. Hronowski ,  Jeffrey K. Pullen

发明人： Milan S. Blake ,  Joseph Y. Tai ,  Huilin L. Qi ,  Shu-Mei Liang ,  Lucjan J.J. Hronowski ,  Jeffrey K. Pullen

IPC分类号： C12N15/09 ,  A61K39/00 ,  A61K39/095 ,  A61P31/04 ,  C07K1/18 ,  C07K14/01 ,  C07K14/22 ,  C12N1/21 ,  C12N15/31 ,  C12N15/70 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  C12R1/36

CPC分类号： C07K14/005 ,  C07K14/22 ,  C12N15/70 ,  A61K39/00 ,  C07K2319/00 ,  C12N2795/10022 ,  Y10S435/849

摘要： The present invention relates, in general, to a method for the high level expression of the outer membrane protein meningococcal group B porin proteins and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein meningococcal group B porin proteins in E. coli wherein the meningococcal group B porin proteins and fusion proteins thereof comprise more than 2% of the total protein expressed in E. coli. The invention also relates to a method of purification and refolding of the meningococcal group B porin proteins and fusion proteins thereof and to their use in vaccines.

摘要翻译： 本发明一般涉及外膜蛋白脑膜炎球菌B族孔蛋白及其融合蛋白的高水平表达的方法。 特别地，本发明涉及一种在大肠杆菌中表达外膜蛋白质脑膜炎球菌B蛋白孔蛋白的方法，其中脑膜炎球菌B族孔蛋白及其融合蛋白包含大肠杆菌中表达的总蛋白的2％以上 。 本发明还涉及脑膜炎球菌B族孔蛋白及其融合蛋白的纯化和重折叠方法及其在疫苗中的应用。