公开(公告)号：US5798233A

公开(公告)日：1998-08-25

申请号：US683458

申请日：1996-07-18

申请人： Emil C. Gotschlich

发明人： Emil C. Gotschlich

IPC分类号： C12N15/09 ,  A61K35/74 ,  A61K39/095 ,  A61P31/04 ,  C07K14/22 ,  C12N1/21 ,  C12N9/10 ,  C12N15/54 ,  C12P19/18 ,  C12R1/36

CPC分类号： C07K14/22 ,  C12N9/1048 ,  C12N9/1051 ,  C12P19/18

摘要： The present invention is directed to nucleic acids encoding glycosyltransferases, the proteins encoded thereby, and to methods for synthesizing oligosaccharides using the glycosyltransferases of the invention. In particular, the present application is directed to identification a glycosyltransferase locus of Neisseria gonorrhoeae containing five open reading frames for five different glycosyltransferases. The functionally active glycosyltransferases of the invention are characterized by catalyzing reactions such as adding Gal .beta.1.fwdarw.4 to GlcNAc or Glc; adding GalNAc or GlcNAc .beta.1.fwdarw.3 to Gal; and adding Gal .alpha.1.fwdarw.4 to Gal. The glycosyltransferases of the invention are particularly suited to the synthesis of the oligosaccharides Gal.beta.1.fwdarw.4GlcNAc .beta.1.fwdarw.3Gal.beta.1.fwdarw.4Glc (a mimic of lacto-N-neotetraose), GalNAc.beta.1.fwdarw.3Gal.beta.l.fwdarw.4GlcNAc.beta.1.fwdarw.3Gal.beta.1.fwdarw.4Glc.beta.1.fwdarw.4 (a mimic ganglioside), and Gal.alpha.1.fwdarw.4Gal.beta.1.fwdarw.4Glc.beta.1.fwdarw.4Hep.fwdarw.R (a mimic of the saccharide portion of globo-glycolipids).

摘要翻译： 本发明涉及编码糖基转移酶的核酸，由此编码的蛋白质，以及使用本发明的糖基转移酶合成寡糖的方法。 特别地，本申请涉及鉴定含有五个不同糖基转移酶的五个开放阅读框的淋病奈瑟氏球菌的糖基转移酶基因座。 本发明的功能活性糖基转移酶的特征在于催化反应，例如向GlcNAc或Glc加入Galβ1-4; 将GalNAc或GlcNAcβ1-> 3加入Gal; 并将Galα1-> 4加入Gal。 本发明的糖基转移酶特别适用于低聚糖Galβ1-> 4GlcNAcβ1->3Galβ1-> 4Glc（模拟乳-N-新四糖），GalNAcβ1->3Galβ1- > 4GlcNAcβ1->3Galβ1-> 4Glcβ1-> 4（模拟神经节苷脂）和Galα1->4Galβ1-> 4Glcβ1-> 4Hep-> R（模拟糖部分 的糖尿病）。

公开(公告)号：US5705367A

公开(公告)日：1998-01-06

申请号：US683426

申请日：1996-07-18

申请人： Emil C. Gotschlich

发明人： Emil C. Gotschlich

IPC分类号： C12N15/09 ,  A61K35/74 ,  A61K39/095 ,  A61P31/04 ,  C07K14/22 ,  C12N1/21 ,  C12N9/10 ,  C12N15/54 ,  C12P19/18 ,  C12R1/36

CPC分类号： C07K14/22 ,  C12N9/1048 ,  C12N9/1051 ,  C12P19/18

摘要： The present invention is directed to nucleic acids encoding glycosyltransferases, the proteins encoded thereby, and to methods for synthesizing oligosaccharides using the glycosyltransferases of the invention. In particular, the present application is directed to identification a glycosyltransferase locus of Neisseria gonorrhoeae containing five open reading frames for five different glycosyltransferases. The functionally active glycosyltransferases of the invention are characterized by catalyzing reactions such as adding Gal .beta..fwdarw.4 to GlcNAc or Glc; adding GalNAc or GlcNAc .beta.1.fwdarw.3 to Gal; and adding Gal .alpha.1.fwdarw.4 to Gal. The glycosyltransferases of the invention are particularly suited to the synthesis of the oligosaccharides Gal.beta.1.fwdarw.4GlcNAc.beta.1.fwdarw.3Gal.beta.1.fwdarw.4Glc (a mimic of lacto-N-neotetraose), GalNAc.beta.1.fwdarw.3Gal.beta.1.fwdarw.4GlcNAc.beta.1.fwdarw.3Gal.beta.1.fwdarw.4Glc.beta.1.fwdarw.4 (a mimic ganglioside), and Gal.alpha.1.fwdarw.4Gal.beta.1.fwdarw.4Glc.beta.1.fwdarw.4Hep.fwdarw.R (a mimic of the saccharide portion of globo-glycolipids).

摘要翻译： 本发明涉及编码糖基转移酶的核酸，由此编码的蛋白质，以及使用本发明的糖基转移酶合成寡糖的方法。 特别地，本申请涉及鉴定含有五个不同糖基转移酶的五个开放阅读框的淋病奈瑟氏球菌的糖基转移酶基因座。 本发明的功能活性糖基转移酶的特征在于催化反应，例如向GlcNAc或Glc加入Galβ→4; 将GalNAc或GlcNAcβ1-> 3加入Gal; 并将Galα1-> 4加入Gal。 本发明的糖基转移酶特别适用于合成低聚糖Galβ1-> 4GlcNAcβ1->3Galβ1-> 4Glc（模拟乳-N-新四糖），GalNAcβ1->3Galβ1- > 4GlcNAcβ1->3Galβ1-> 4Glcβ1-> 4（模拟神经节苷脂）和Galα1->4Galβ1-> 4Glcβ1-> 4Hep-> R（模拟糖部分 的糖尿病）。

公开(公告)号：US5550040A

公开(公告)日：1996-08-27

申请号：US214861

申请日：1994-03-17

申请人： Ashok P. Purohit ,  Sheryl B. Silver

发明人： Ashok P. Purohit ,  Sheryl B. Silver

IPC分类号： C07H21/04 ,  C12N15/09 ,  C12Q1/68 ,  C12R1/36 ,  C12P19/34 ,  C07H21/02 ,  C12N15/00

CPC分类号： C12Q1/689 ,  Y10S435/81

摘要： Methods, reagents and kits are provided for simultaneously amplifying and detecting polynucleotide sequences in bacteria causing Neisseria gonorrhoeae and/or Chlamydia trachomatis using primers and probes specific for each bacterial species.

摘要翻译： 提供方法，试剂和试剂盒，用于使用针对每种细菌物种特异的引物和探针同时扩增和检测导致淋病奈瑟氏球菌和/或沙眼衣原体的细菌中的多核苷酸序列。

公开(公告)号：US5139942A

公开(公告)日：1992-08-18

申请号：US196028

申请日：1988-05-19

申请人： Jack S. Benner

发明人： Jack S. Benner

IPC分类号： C12N9/16 ,  C12N1/21 ,  C12N9/10 ,  C12N9/22 ,  C12N15/09 ,  C12N15/54 ,  C12N15/55 ,  C12R1/19 ,  C12R1/36

CPC分类号： C12N9/22 ,  C12N9/1007

摘要： The present invention is directed to a method for cloning and producing the Nde I restriction endonuclease by 1) introducing the restriction endonuclease gene from Neisseria denitrificans into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the vector encoding and expressing the Nde I restriction endonuclease, and 3) purifying the Nde I restriction endonuclease from the fermented host which contains the vector encoding and expressing the Nde I restriction endonuclease activity.

公开(公告)号：US5075232A

公开(公告)日：1991-12-24

申请号：US225246

申请日：1988-07-28

申请人： Richard D. Morgan

发明人： Richard D. Morgan

IPC分类号： C12N1/21 ,  C12N9/10 ,  C12N9/16 ,  C12N9/22 ,  C12N15/54 ,  C12N15/55 ,  C12R1/19 ,  C12R1/36

CPC分类号： C12N9/22 ,  C12N9/1007

摘要： The present invention is directed to a method for cloning and producing the NlaIV restriction endonuclease by 1) introducing the restriction endonuclease gene from N. lactamica NRCC 2118 into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the vector encoding and expressing the NlaIV restriction endonuclease, and 3) purifying the NlaIV restriction endonuclease from the fermented host which contains the vector encoding and expressing the NlaIV restriction endonuclease activity.

公开(公告)号：US5075221A

公开(公告)日：1991-12-24

申请号：US255921

申请日：1988-10-07

申请人： John C. Mauck ,  Robert W. Zercie

发明人： John C. Mauck ,  Robert W. Zercie

IPC分类号： C08K5/00 ,  C08L101/00 ,  C12N9/50 ,  C12P21/00 ,  C12R1/01 ,  C12R1/36 ,  G01N33/569 ,  G01N33/571

CPC分类号： C12N9/50 ,  G01N33/56927 ,  G01N33/571 ,  Y10S436/826 ,  Y10T436/2525

摘要： A composition including a sulfhydryl-containing reducing agent is stabilized for long term storage with a hydrophilic polymer. This composition can be combined with one or more reagents useful for extracting antigens from chlamydial or gonococcal organisms. The extracted antigen can be determined using immunological reactions and appropriate labeled reactants.

摘要翻译： 包含含巯基还原剂的组合物被稳定以用亲水性聚合物长期保存。 该组合物可以与一种或多种用于从衣原体或淋球菌生物体提取抗原的试剂组合。 提取的抗原可以使用免疫反应和合适的标记反应物来测定。

公开(公告)号：US4795803A

公开(公告)日：1989-01-03

申请号：US817849

申请日：1986-02-19

申请人： Frederick C. Lindberg ,  Bjorn O. Lund ,  Britt M. Baga ,  Mari E. Norgren ,  Mikael Goransson ,  Bernt E. Uhlin ,  Jan S. Normark ,  David L. Lark

发明人： Frederick C. Lindberg ,  Bjorn O. Lund ,  Britt M. Baga ,  Mari E. Norgren ,  Mikael Goransson ,  Bernt E. Uhlin ,  Jan S. Normark ,  David L. Lark

IPC分类号： G01N33/531 ,  A61K20060101 ,  A61K39/00 ,  A61K39/02 ,  A61K39/108 ,  A61P31/04 ,  C07H20060101 ,  C07H21/04 ,  C07K20060101 ,  C07K14/00 ,  C07K14/195 ,  C07K14/21 ,  C07K14/22 ,  C07K14/235 ,  C07K14/24 ,  C07K14/245 ,  C07K14/31 ,  C07K14/315 ,  C07K14/41 ,  C07K16/00 ,  C07K16/12 ,  C12N20060101 ,  C12N5/00 ,  C12N5/10 ,  C12N15/00 ,  C12N15/09 ,  C12N15/31 ,  C12N15/62 ,  C12P20060101 ,  C12P21/00 ,  C12P21/02 ,  C12P21/08 ,  C12R1/01 ,  C12R1/125 ,  C12R1/19 ,  C12R1/36 ,  C12R1/38 ,  C12R1/44 ,  C12R1/46 ,  G01N33/569 ,  C07K15/00 ,  C07K15/04

CPC分类号： C07K14/24 ,  C07K14/21 ,  C07K14/22 ,  C07K14/235 ,  C07K14/245 ,  C07K14/31 ,  C07K14/315 ,  A61K39/00 ,  C07K2319/00 ,  C07K2319/33 ,  C07K2319/40 ,  Y10S435/82 ,  Y10S530/806 ,  Y10S530/825

摘要： An antigen which, as its major immunizing component, comprises a determinant of an adhesin polypeptide or an immunogenically active subsequence thereof or a precursor therefor which is convertible to an immunogenically active form, antibodies against which determinant react with the adhesion polypeptide produced by pathogenic adhesin-forming bacteria which adhere to mammalian tissue, antibodies against such antigen, and DNA expressing, as a principal gene product thereof, such antigen.

摘要翻译： PCT No.PCT / DK85 / 00045 Sec。 371日期1986年2月19日 102（e）日期1986年2月19日PCT提交1985年5月2日PCT公布。 第WO85 / 05037号公报 日期：1985年11月21日。作为其主要免疫组分的抗原包含可转化为免疫原性活性形式的粘附素多肽或其免疫原性活性亚序列或其前体的决定簇，其决定簇与 由附着于哺乳动物组织的病原性粘附素形成细菌产生的粘附多肽，抗这种抗原的抗体，以及表达作为其主要基因产物的DNA。

公开(公告)号：US4539201A

公开(公告)日：1985-09-03

申请号：US546600

申请日：1983-10-28

申请人： Robert M. Gwin

发明人： Robert M. Gwin

IPC分类号： A61K39/095 ,  A61K39/02 ,  C12R1/36 ,  C12R1/365

CPC分类号： A61K39/095 ,  A61K39/1045 ,  A61K2039/552 ,  Y10S424/823 ,  Y10S435/871 ,  Y10S435/872

摘要： A medicament and method for inducing immunity in to infectious bovine keratoconjunctivitis in cattle. The medicament comprises the gram negative cocci Neisseria or Branhamella which are non-etiological agents of infectious Keratoconjunctivitis yet unexpectedly are found to afford an immunity to infectious bovine keratoconjunctivitis when administered to cattle.

摘要翻译： 用于诱导牛中感染性牛角膜结膜炎的免疫的药物和方法。 药物包括革兰氏阴性球菌奈瑟氏球菌或布兰汉菌属，它们是传染性角膜结膜炎的非病原体，但是当被给予牛时，意外地发现可以感染牛角膜结膜炎免疫。

公开(公告)号：US11292808B2

公开(公告)日：2022-04-05

申请号：US16072895

申请日：2017-01-27

申请人： De Staat der Nederlanden, vert. door de minister van VWS, Ministerie van Volksgezondheid, Welzijn en Sport

发明人： Afshin Zariri ,  Elder Pupo Escalona ,  Peter André Van Der Ley

IPC分类号： C07H15/06 ,  C12N1/20 ,  A61K39/095 ,  A61P31/04 ,  A61K31/7024 ,  C12R1/36

摘要： The present invention relates to neisserial LPS having a hexa-acylated lipid A moiety, wherein the hexa-acylated lipid A moiety is modified as compared to the lipid A moiety of a wild-type neisserial LPS in that it comprises a palmitoleoyl instead of a lauroyl secondary acyl chain on the glucosamine at the non-reducing end of the lipid A moiety. The invention further relates to mixtures of the hexa-acylated LPS with the corresponding penta-acylated LPS, lacking a secondary acyl chain on the glucosamine at the non-reducing end of the lipid A moiety. The invention also relates to neisserial bacteria that have been genetically modified to reduce expression of the endogenous lpxL1 gene and to introduce expression of a heterologous thermosensitive lpxP gene for producing the hexa- and penta-acylated LPS. By selecting the time and/or temperature at which the bacterium is grown, it is feasible to increase or decrease the amount of hexa-acylated lipid A structure relative to the corresponding penta-acylated structure and thereby modulate the TLR4 agonist activity of the neisserial LPS of the invention, to the exact level of activity required for a particular immunotherapeutic approach.

公开(公告)号：US10881721B2

公开(公告)日：2021-01-05

申请号：US16716638

申请日：2019-12-17

申请人： GLAXOSMITHKLINE BIOLOGICALS SA

发明人： Lisa Danzig

IPC分类号： A61K39/095 ,  A61K47/64 ,  C07K14/22 ,  C07K16/12 ,  A61K39/116 ,  A61K39/00 ,  C12R1/36

摘要： Multivalent meningococcal conjugate vaccines are administered according to a schedule in which a first dose is administered to a patient aged between 0 and 12 months, and a second dose is administered to the patient aged between 12 and 24 months.