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1.发明授权公开(公告)号：US07122304B2
公开(公告)日：2006-10-17
申请号：US09725897
申请日：2000-11-30
CPC分类号： C12N15/1096 , C12Q1/6806 , C12Q2565/625
摘要： The invention relates to storage of nucleic acid (particularly mRNA) on a solid support and to using such nucleic acid in nucleic acid synthesis or amplification reactions. In a preferred aspect, the invention provides synthesis of cDNA and cDNA libraries.
摘要翻译： 本发明涉及在固体支持物上存储核酸（特别是mRNA），并且在核酸合成或扩增反应中使用这种核酸。 在优选的方面，本发明提供cDNA和cDNA文库的合成。
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公开(公告)号：US07833705B2
公开(公告)日：2010-11-16
申请号：US10600584
申请日：2003-06-19
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806
摘要： The invention relates to a solid medium or matrix for storage of nucleic acid molecules (e.g. RNA and/or DNA), particularly vectors and especially plasmids, comprising a solid matrix preferably having a compound or composition which protects against degradation of nucleic acids incorporated into or absorbed on the matrix. The invention also relates to methods for storage or isolation/purification of nucleic acids using this solid medium, and in situ use of the stored or isolated/purified nucleic acids.
摘要翻译： 本发明涉及用于储存核酸分子（例如RNA和/或DNA）的固体培养基或基质，特别是载体，特别是质粒，其包含固体基质，优选具有保护免于降解结合到或 吸收在矩阵上。 本发明还涉及使用该固体培养基存储或分离/纯化核酸的方法，以及原位使用所储存或分离/纯化的核酸。
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3.发明授权Compositions and methods for the release of nucleic acid molecules from solid matrices 失效用于从固体基质释放核酸分子的组合物和方法公开(公告)号：US06803200B2
公开(公告)日：2004-10-12
申请号：US10012443
申请日：2001-12-12
IPC分类号： C12Q168
CPC分类号： C12N15/1017 , C12N15/1006 , C12Q1/6806 , C12Q2527/125
摘要： The present invention relates to compositions and methods for releasing nucleic acid molecules from solid matrices. The invention further relates to compositions and methods for purifying and isolating nucleic acid molecules from biological materials such as animal tissues and plant matter. The methods of the invention can be readily adapted for rapid processing of multiple samples. Thus, the invention further provides automated methods for the purification of nucleic acid molecules from numerous samples. The invention also relates to kits for removing nucleic acid molecules from solid matrices.
摘要翻译： 本发明涉及从固体基质中释放核酸分子的组合物和方法。 本发明还涉及从生物材料如动物组织和植物物质中纯化和分离核酸分子的组合物和方法。 本发明的方法可以容易地适用于多个样品的快速处理。 因此，本发明还提供了用于从许多样品纯化核酸分子的自动化方法。 本发明还涉及从固体基质中除去核酸分子的试剂盒。
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公开(公告)号：US06750059B1
公开(公告)日：2004-06-15
申请号：US09354664
申请日：1999-07-16
IPC分类号： C12N1564
CPC分类号： C12Q1/6806
摘要： The invention relates to a solid medium or matrix for storage of nucleic acid molecules (e.g. RNA and/or DNA), particularly vectors and especially plasmids, comprising a solid matrix preferably having a compound or composition which protects against degradation of nucleic acids incorporated into or absorbed on the matrix. The invention also relates to methods for storage or isolation/purification of nucleic acids using this solid medium, and in situ use of the stored or isolated/purified nucleic acids.
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