公开(公告)号：US07435603B2

公开(公告)日：2008-10-14

申请号：US11249683

申请日：2005-10-12

申请人： Mingde Zhu ,  Lee Olech

发明人： Mingde Zhu ,  Lee Olech

IPC分类号： G01N33/533 ,  C07D309/00 ,  C07H23/00

CPC分类号： G01N33/54366 ,  G01N33/6839 ,  G01N2021/6439 ,  Y10S435/968 ,  Y10S436/80 ,  Y10T436/13

摘要： The proteins in a biological sample that is sought to be analyzed for its protein composition by an electrophoretic or chromatographic procedure are coupled to a dye in an unusually efficient manner by combining the sample with a solid dry composition containing the dye, a buffering agent, and in preferred embodiments, a denaturing agent as well. The solid and dry form of the composition prevents the dye from deteriorating or decomposing, and the combination of components in the composition allows the dye to couple to the proteins in a relatively uniform manner with no overstaining of the protein when the composition and the sample are heated together and held at an elevated temperature for a short period of time.

摘要翻译： 通过电泳或色谱方法试图分析其蛋白质组成的生物样品中的蛋白质通过将样品与含有染料，缓冲剂和固体干燥组合物的固体干燥组合物以非常有效的方式偶联到染料上 在优选的实施方案中，还可以使用变性剂。 组合物的固体和干燥形式可防止染料劣化或分解，组合物中组分的组合允许染料以相对均匀的方式偶联蛋白质，当组合物和样品为 一起加热并在高温下保持短时间。

公开(公告)号：US20060052279A1

公开(公告)日：2006-03-09

申请号：US11249683

申请日：2005-10-12

申请人： Mingde Zhu ,  Lee Olech

发明人： Mingde Zhu ,  Lee Olech

IPC分类号： A61K31/00 ,  C07K1/26

CPC分类号： G01N33/54366 ,  G01N33/6839 ,  G01N2021/6439 ,  Y10S435/968 ,  Y10S436/80 ,  Y10T436/13

摘要： The proteins in a biological sample that is sought to be analyzed for its protein composition by an electrophoretic or chromatographic procedure are coupled to a dye in an unusually efficient manner by combining the sample with a solid dry composition containing the dye, a buffering agent, and in preferred embodiments, a denaturing agent as well. The solid and dry form of the composition prevents the dye from deteriorating or decomposing, and the combination of components in the composition allows the dye to couple to the proteins in a relatively uniform manner with no overstaining of the protein when the composition and the sample are heated together and held at an elevated temperature for a short period of time.

公开(公告)号：US06995023B2

公开(公告)日：2006-02-07

申请号：US10278745

申请日：2002-10-21

申请人： Mingde Zhu ,  Lee Olech

发明人： Mingde Zhu ,  Lee Olech

IPC分类号： G01N21/76

CPC分类号： G01N33/54366 ,  G01N33/6839 ,  G01N2021/6439 ,  Y10S435/968 ,  Y10S436/80 ,  Y10T436/13

摘要： The proteins in a biological sample that is sought to be analyzed for its protein composition by an electrophoretic or chromatographic procedure are coupled to a dye in an unusually efficient manner by combining the sample with a solid dry composition containing the dye, a buffering agent, and in preferred embodiments, a denaturing agent as well. The solid and dry form of the composition prevents the dye from deteriorating or decomposing, and the combination of components in the composition allows the dye to couple to the proteins in a relatively uniform manner with no overstaining of the protein when the composition and the sample are heated together and held at an elevated temperature for a short period of time.

摘要翻译： 通过电泳或色谱方法试图分析其蛋白质组成的生物样品中的蛋白质通过将样品与含有染料，缓冲剂和固体干燥组合物的固体干燥组合物以非常有效的方式偶联到染料上 在优选的实施方案中，还可以使用变性剂。 组合物的固体和干燥形式可防止染料劣化或分解，组合物中组分的组合允许染料以相对均匀的方式偶联蛋白质，当组合物和样品为 一起加热并在高温下保持短时间。

公开(公告)号：US20050287674A1

公开(公告)日：2005-12-29

申请号：US11196148

申请日：2005-08-02

申请人： Mingde Zhu ,  Lee Olech

发明人： Mingde Zhu ,  Lee Olech

IPC分类号： G01N33/58 ,  G01N21/76 ,  G01N21/78 ,  G01N27/447 ,  G01N33/483 ,  G01N33/543 ,  G01N33/68

CPC分类号： G01N33/54366 ,  G01N33/6839 ,  G01N2021/6439 ,  Y10S435/968 ,  Y10S436/80 ,  Y10T436/13

摘要： The proteins in a biological sample that is sought to be analyzed for its protein composition by an electrophoretic or chromatographic procedure are coupled to a dye in an unusually efficient manner by combining the sample with a solid dry composition containing the dye a buffering agent, and in preferred embodiments, a denaturing agent as well. The solid and dry form of the composition prevents the dye from deteriorating or decomposing, and the combination of components in the composition allows the dye to couple to the proteins in a relatively uniform manner with no overstaining of the protein when the composition and the sample are heated together and held at an elevated temperature for a short period of time.

摘要翻译： 通过电泳或色谱方法试图分析其蛋白质组成的生物样品中的蛋白质通过将样品与含有染料的固体干燥组合物缓冲剂以非常有效的方式偶联到染料中，并且 优选的实施方案，变性剂。 组合物的固体和干燥形式可防止染料劣化或分解，组合物中组分的组合允许染料以相对均匀的方式偶联蛋白质，当组合物和样品为 一起加热并在高温下保持短时间。

公开(公告)号：US20120285830A1

公开(公告)日：2012-11-15

申请号：US13555944

申请日：2012-07-23

申请人： Craig Rowell ,  Cory Panattoni ,  Thomas R. Berkelman ,  Sean Cater ,  Shane Petersen ,  Lee Olech ,  Xuemei Yang

发明人： Craig Rowell ,  Cory Panattoni ,  Thomas R. Berkelman ,  Sean Cater ,  Shane Petersen ,  Lee Olech ,  Xuemei Yang

IPC分类号： C07K1/26

CPC分类号： G01N27/44747 ,  B01D57/02 ,  C08F220/56 ,  C08F222/385

摘要： Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

摘要翻译： 聚丙烯酰胺凝胶在蛋白质分离中提供高分辨率并且相对于水解而言比常规聚丙烯酰胺凝胶更稳定，其依赖于Tris或Tris-Bis作为缓冲剂，通过掺入三乙醇胺代替大部分或全部的Tris或Tris-Bis制备。

公开(公告)号：US20100051462A1

公开(公告)日：2010-03-04

申请号：US12552104

申请日：2009-09-01

申请人： Craig Rowell ,  Cory Panattoni ,  Thomas R. Berkelman ,  Sean Cater ,  Shane Petersen ,  Lee Olech ,  Xuemei Yang

发明人： Craig Rowell ,  Cory Panattoni ,  Thomas R. Berkelman ,  Sean Cater ,  Shane Petersen ,  Lee Olech ,  Xuemei Yang

IPC分类号： G01N33/559 ,  B01D57/02

CPC分类号： G01N27/44747 ,  B01D57/02 ,  C08F220/56 ,  C08F222/385

摘要： Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

摘要翻译： 聚丙烯酰胺凝胶在蛋白质分离中提供高分辨率并且相对于水解而言比常规聚丙烯酰胺凝胶更稳定，其依赖于Tris或Tris-Bis作为缓冲剂，通过掺入三乙醇胺代替大部分或全部的Tris或Tris-Bis制备。

公开(公告)号：US07384528B2

公开(公告)日：2008-06-10

申请号：US10746453

申请日：2003-12-23

申请人： Cory M. Panattoni ,  Lee Olech

发明人： Cory M. Panattoni ,  Lee Olech

IPC分类号： G01N27/453

CPC分类号： G01N27/44704

摘要： In pre-cast slab gel cassettes, the formation of pathways in which proteins can migrate between the gel and the walls of the cassette to form shadow bands is avoided by the application of a coating of a nonionic amphiphilic polymer to the cassette walls. The coating also prevents the gel from sticking to the walls when the gel is to be removed from the cassette after electrophoresis.

摘要翻译： 在预制板坯凝胶盒中，通过将非离子两亲聚合物的涂层施加到盒壁上，避免蛋白质可以在胶体与盒壁之间迁移以形成阴影带的途径的形成。 当凝胶在电泳后要从盒中取出时，涂层也可以防止凝胶粘到壁上。

公开(公告)号：US08506781B2

公开(公告)日：2013-08-13

申请号：US13555944

申请日：2012-07-23

申请人： Craig Rowell ,  Cory Panattoni ,  Tom R Berkelman ,  Sean Cater ,  Shane Petersen ,  Lee Olech ,  Xuemei Yang

发明人： Craig Rowell ,  Cory Panattoni ,  Tom R Berkelman ,  Sean Cater ,  Shane Petersen ,  Lee Olech ,  Xuemei Yang

IPC分类号： G01N27/447 ,  C07K1/26

CPC分类号： G01N27/44747 ,  B01D57/02 ,  C08F220/56 ,  C08F222/385

摘要： Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

摘要翻译： 聚丙烯酰胺凝胶在蛋白质分离中提供高分辨率并且相对于水解而言比常规聚丙烯酰胺凝胶更稳定，其依赖于Tris或Tris-Bis作为缓冲剂，通过掺入三乙醇胺代替大部分或全部的Tris或Tris-Bis制备。

公开(公告)号：US20050074895A1

公开(公告)日：2005-04-07

申请号：US10931943

申请日：2004-08-31

申请人： Lee Olech

发明人： Lee Olech

IPC分类号： C07K14/47 ,  C12Q20060101 ,  G01N33/00 ,  G01N33/20 ,  G01N33/68

CPC分类号： G01N33/6848 ,  Y10T436/142222

摘要： Comparative studies of proteins from two or more cell populations are performed by alkylating the cysteine thiol groups of the proteins of all populations with vinyl pyridines in which the pyridine ring in at least one of the populations, in addition to the vinyl substituent, bears one or more alkyl substituents, the various populations thus differing in the alkyl substituents on the pyridine ring. Proteins of one population are thus alkylated with vinyl pyridine itself and those of another population with an alkyl-substituted vinyl pyridine, or both with alkyl-substituted vinyl pyridines using different alkyl groups or a different number of alkyl groups. The mass differential afforded by the difference in alkyl substitution affords a means of pairing individual proteins of one population with those of another while differentiating between the two, using analytical techniques disclosed in the prior art for isotope-coded affinity tagging.

摘要翻译： 来自两个或多个细胞群体的蛋白质的比较研究是通过用乙烯基吡啶烷基化所有群体的蛋白质的半胱氨酸硫醇基团进行的，其中至少一个群体中的吡啶环除了乙烯基取代基之外，还含有一个或 更多的烷基取代基，因此各种群体在吡啶环上的烷基取代基不同。 因此，一个群体的蛋白质由乙烯基吡啶本身和另一个群体与烷基取代的乙烯基吡啶烷基化，或两者使用不同烷基或不同数目的烷基烷基取代的乙烯基吡啶二烷基化。 通过烷基取代的差异提供的质量差异提供了使用现有技术中公开的用于同位素编码的亲和标记的分析技术，将一个群体的单个蛋白质与另一个群体的单个蛋白质与两者之间的差异进行分化的方法。

公开(公告)号：US07022214B2

公开(公告)日：2006-04-04

申请号：US10763405

申请日：2004-01-21

申请人： Lee Olech

发明人： Lee Olech

IPC分类号： G01N27/26 ,  C07C279/00 ,  C07C279/12

CPC分类号： G01N27/44795 ,  C07C279/12 ,  G01N27/44747

摘要： Carboxylic acid-substituted polyalkylene polyamines in which amine nitrogen atoms on the polyamine backbone structure are replaced by guanidine groups provide a pH range extending into high pH values. These substances are useful as carrier ampholytes in isoelectric focusing.

摘要翻译： 羧胺取代的聚亚烷基多胺，其中多胺骨架结构上的胺氮原子被胍基团取代，提供了延伸到高pH值的pH范围。 这些物质可用作等电聚焦中的载体两性电解质。