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公开(公告)号：US08137736B2
公开(公告)日：2012-03-20
申请号：US12348589
申请日：2009-01-05
申请人： Mingwei Zhu , Yanfeng Chen , Zhenlin Wang , Yanqing Lu , Haixiong Ge , Yuefeng Tang
发明人： Mingwei Zhu , Yanfeng Chen , Zhenlin Wang , Yanqing Lu , Haixiong Ge , Yuefeng Tang
IPC分类号： B05D3/12
CPC分类号： B81C1/00476 , A61M37/0015 , A61M2037/003 , A61M2037/0053 , B81B2201/055 , B81B2203/0338 , B81B2203/0361 , B81C2201/0108
摘要： A novel method suitable for commercially mass production of hollow microneedle with high quality for delivery of drugs across or into biological tissue is provided. It typically includes the following processes: (1) coating an elongated template of a first material with a second material to form a cover; (2) removing tips of the template and cover to form an opening in the cover; and (3) removing the template of the first material to obtain hollow microneedles of the second material. This simple, efficient and cost-effective fabrication method can mass produce hollow microneedle arrays involving no complicated and expensive equipments or techniques, which can be used in commercial fabrication of hollow needles for delivering drugs or genes across or into skin or other tissue barriers with advantages of minimal damage, painless, long-term and continuous usages.
摘要翻译： 提供了一种适用于商业上大规模生产用于将药物跨越或进入生物组织的高品质的中空微针的新方法。 它通常包括以下过程：（1）用第二材料涂覆第一材料的细长模板以形成盖子; （2）去除模板和盖子的尖端以在盖子上形成开口; 和（3）去除第一材料的模板以获得第二材料的中空微针。 这种简单，高效和成本有效的制造方法可以大规模生产中空微针阵列，其不涉及复杂而昂贵的设备或技术，其可用于商业制造用于将药物或基因跨越或进入皮肤或其它组织屏障的中空针 最小的伤害，无痛，长期和持续的使用。
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公开(公告)号：US20100062142A1
公开(公告)日：2010-03-11
申请号：US12348589
申请日：2009-01-05
申请人： Mingwei Zhu , Yanfeng Chen , Zhenlin Wang , Yanqing Lu , Haixiong Ge , Yuefeng Tang
发明人： Mingwei Zhu , Yanfeng Chen , Zhenlin Wang , Yanqing Lu , Haixiong Ge , Yuefeng Tang
IPC分类号： B05D3/00
CPC分类号： B81C1/00476 , A61M37/0015 , A61M2037/003 , A61M2037/0053 , B81B2201/055 , B81B2203/0338 , B81B2203/0361 , B81C2201/0108
摘要： A novel method suitable for commercially mass production of hollow microneedle with high quality for delivery of drugs across or into biological tissue is provided. It typically includes the following processes: (1) coating an elongated template of a first material with a second material to form a cover; (2) removing tips of the template and cover to form an opening in the cover; and (3) removing the template of the first material to obtain hollow microneedles of the second material. This simple, efficient and cost-effective fabrication method can mass produce hollow microneedle arrays involving no complicated and expensive equipments or techniques, which can be used in commercial fabrication of hollow needles for delivering drugs or genes across or into skin or other tissue barriers with advantages of minimal damage, painless, long-term and continuous usages.
摘要翻译： 提供了一种适用于商业上大规模生产用于将药物跨越或进入生物组织的高品质的中空微针的新方法。 它通常包括以下过程：（1）用第二材料涂覆第一材料的细长模板以形成盖子; （2）去除模板和盖子的尖端以在盖子上形成开口; 和（3）去除第一材料的模板以获得第二材料的中空微针。 这种简单，高效和成本有效的制造方法可以大规模生产中空微针阵列，其不涉及复杂而昂贵的设备或技术，其可用于商业制造用于将药物或基因跨越或进入皮肤或其它组织屏障的中空针 最小的伤害，无痛，长期和持续的使用。
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公开(公告)号：US20110306751A1
公开(公告)日：2011-12-15
申请号：US13122547
申请日：2009-10-05
CPC分类号： C12N15/72 , C07K14/31 , C07K2319/02 , C12N1/20 , C12N9/22 , C12N15/635 , C12N15/67 , C12Y301/00 , H05K999/99
摘要： The present invention is directed to the improved methods for the temporal induction of proteins using the condensed single protein production (cSPP) system.
摘要翻译： 本发明涉及使用缩合单蛋白质生产（cSPP）系统来时间诱导蛋白质的改进方法。
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4.发明申请Labeled Biomolecular Compositions and Methods for the Production and Uses Thereof 有权标记的生物分子组合物及其生产和使用方法公开(公告)号：US20140011228A1
公开(公告)日：2014-01-09
申请号：US13902228
申请日：2013-05-24
CPC分类号： C12P21/00 , C12N9/22 , C12N15/635 , C12P21/02 , G01N24/087 , G01N33/534 , G01N33/60 , G01N2333/922 , Y02P20/52
摘要： Disclosed herein is a set of E. coli single-protein production (SPP) technologies with protein NMR (SPP-NMR) for (i) using isotope-enriched membrane proteins produced with the SPP system in screening detergent conditions suitable for purification and/or three-dimensional structure analysis without the requirement for protein purification, (ii) producing 2H, 13C, 15N enriched proteins suitable for high throughput and membrane protein NMR studies, and (iii) labeling with 13C—15N specific peptide bonds in proteins (referred to herein as SPP-PBL).
摘要翻译： 本文公开了一组具有蛋白质NMR（SPP-NMR）的大肠杆菌单蛋白生产（SPP）技术，用于（i）使用在SPP系统中产生的同位素富集的膜蛋白筛选适合于纯化的洗涤剂条件和/或 不需要蛋白质纯化的三维结构分析，（ii）生产适合于高通量和膜蛋白质NMR研究的2H，13C，15N富集的蛋白质，和（iii）用蛋白质中13C-15N特异性肽键进行标记（参考 本文作为SPP-PBL）。
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5.发明授权Labeled biomolecular compositions and methods for the production and uses thereof 有权标记的生物分子组合物及其制备方法和用途公开(公告)号：US08450085B2
公开(公告)日：2013-05-28
申请号：US12531447
申请日：2008-03-17
IPC分类号： C12P21/06
CPC分类号： C12P21/00 , C12N9/22 , C12N15/635 , C12P21/02 , G01N24/087 , G01N33/534 , G01N33/60 , G01N2333/922 , Y02P20/52
摘要： Disclosed herein is a set of E. coli single-protein production (SPP) technologies with protein NMR (SPP-NMR) for (i) using isotope-enriched membrane proteins produced with the SPP system in screening detergent conditions suitable for purification and/or three-dimensional structure analysis without the requirement for protein purification, (ii) producing 2H, 13C, 15N enriched proteins suitable for high throughput and membrane protein NMR studies, and (iii) labeling with 13C—15N specific peptide bonds in proteins (referred to herein as SPP-PBL).
摘要翻译： 本文公开了一组具有蛋白质NMR（SPP-NMR）的大肠杆菌单蛋白生产（SPP）技术，用于（i）使用在SPP系统中产生的同位素富集的膜蛋白筛选适合于纯化的洗涤剂条件和/或 不需要蛋白质纯化的三维结构分析，（ii）生产适合于高通量和膜蛋白质NMR研究的2H，13C，15N富集的蛋白质，和（iii）用蛋白质中13C-15N特异性肽键进行标记（参考 本文作为SPP-PBL）。
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