公开(公告)号：US20020194634A1

公开(公告)日：2002-12-19

申请号：US10068460

申请日：2002-02-06

Applicant: Minos Biosystems

Inventor： Roger Craig ,  Charalambos Savakis

IPC: A01K067/033 ,  A01N043/40 ,  A01N057/00

CPC classification number: A01K67/033 ,  A01N63/00 ,  A01N63/02 ,  C07K14/43595 ,  C12N9/78 ,  C12N15/70 ,  C12N15/85 ,  C12N2800/90 ,  C12N2830/002 ,  C12N2830/75

Abstract: A method for controlling a population of target insects comprising providing a gene comprising a coding sequence encoding one constituent of an enzyme/pro-pesticide system and a regulatory region operatively linked to said coding sequence. The regulatory region of the present invention regulates expression of said coding sequence in a target insect of said population of target insects in a sex specific manner. The method of the present invention further comprises transforming a plurality of said target insects with said gene, and allowing said gene to spread within the target insect population. The method further comprises administering to said population of target insects the remaining constituent(s) of said enzyme/pro-pesticide system wherein said enzyme catalyzes the conversion of the pro-pesticide to a pesticide.

Abstract translation: 一种用于控制目标昆虫群体的方法，包括提供包含编码酶/前 - 农药系统的一个成分的编码序列和与所述编码序列有效连接的调节区的基因。 本发明的调节区域以性别特异性方式调节所述编码序列在所述目标昆虫种群的目标昆虫中的表达。 本发明的方法还包括用所述基因转化多个所述目标昆虫，并允许所述基因在目标昆虫群体内扩散。 所述方法还包括向所述群体施用所述酶/前 - 农药系统的剩余组分，其中所述酶催化所述前农药转化为农药。

公开(公告)号：US20030037346A1

公开(公告)日：2003-02-20

申请号：US10124903

申请日：2002-04-18

Applicant: Minos Biosystems

Inventor： Roger Craig ,  Charalambos Savakis

IPC: A01K067/033 ,  C07K014/435

CPC classification number: C12P21/02 ,  A01K67/0333 ,  A01K67/0339 ,  A01K2217/05 ,  C07K14/47 ,  C07K14/61 ,  C12N9/22 ,  C12N9/2408 ,  C12N15/90 ,  C12Y302/0102

Abstract: The intention relates to a method for producing a protein of interest, comprising transforming an insect with a non-viral expression system capable of expressing the protein of interest in the larvae of the insect; breeding the insect to produce larvae; culturing the larvae; and isolating the protein of interest from the larvae. The transformation is advantageously performed by means of a transposon vector.

Abstract translation: 本发明涉及生产目的蛋白质的方法，包括用能够在昆虫幼虫中表达目的蛋白质的非病毒表达系统转化昆虫; 养殖昆虫以产生幼虫; 培养幼虫; 并从幼虫中分离出目的蛋白质。 转化有利地通过转座子载体进行。

公开(公告)号：US20040092018A1

公开(公告)日：2004-05-13

申请号：US10634314

申请日：2003-08-05

Applicant: Minos Biosystems Limited

Inventor： Charalambos Savakis ,  Frank Grosveld

IPC: C12N015/86 ,  A01K067/00

CPC classification number: C12N15/102 ,  C12N15/90 ,  C12N2800/30 ,  C12N2800/60 ,  C12N2800/90 ,  C12N2830/003 ,  C12N2830/205

Abstract: The inventon provides a method for producing a library of genetic mutations in a cell population by insertional mutagenesis, wherein a viral vector comprising a transposon is used to deliver said transposon to said cell population, which cell population stably expresses the cognate transposase for said transposon, and the transposon is mobilised to give a rise to the genetic mutations.

Abstract translation: 本发明提供了一种用于通过插入诱变在细胞群体中产生遗传突变文库的方法，其中包含转座子的病毒载体用于将所述转座子递送至所述细胞群体，所述细胞群稳定表达所述转座子的同源转座酶， 并且转座子被动员以引起遗传突变。

公开(公告)号：US20040003429A1

公开(公告)日：2004-01-01

申请号：US10385768

申请日：2003-03-11

Applicant: Minos Biosystems Limited

Inventor： Roger Craig ,  Charalambos Savakis

IPC: A01H001/00 ,  C12N015/82 ,  A01N057/00 ,  A01N043/40

CPC classification number: C12N15/8286 ,  A01K2217/05 ,  A01N63/00 ,  C12N9/14 ,  C12N15/8279 ,  Y02A40/162 ,  A01N2300/00

Abstract: The present invention provides a method for controlling a population of target pests, comprising: a) providing gene comprising a coding sequence encoding one constituent of an enzyme/pro-pesticide system and a promoter capable of driving the coding sequence in the target plants or vertebrate host; b) transforming the target plant or vertebrate with the gene; and c) administering to the transformed population of target plant or vertebrates the remaining constituents of the enzyme/pro-pesticide system such that inactive pro-pesticide is converted to active pesticide on or within the transformed plant or vertebrate host leading to the death of any invading population of target pests.

Abstract translation: 本发明提供了一种控制目标害虫群体的方法，包括：a）提供基因，其包含编码酶/前农药体系的一个成分的编码序列和能够驱动靶植物或脊椎动物中编码序列的启动子 主办; b）用该基因转化靶植物或脊椎动物; 并且c）向目标植物或脊椎动物的转化群体施用酶/前农药体系的剩余成分，使得在转化的植物或脊椎动物宿主或脊椎动物宿主中或其内的无活性的农药转化为活性农药导致任何 入侵病虫害群体。