公开(公告)号：US09291572B2

公开(公告)日：2016-03-22

申请号：US13441630

申请日：2012-04-06

申请人： Mitsuhiro Oura ,  Hirotsugu Kubo ,  Sunao Takeda ,  Teruo Okano ,  Tatsuya Shimizu

发明人： Mitsuhiro Oura ,  Hirotsugu Kubo ,  Sunao Takeda ,  Teruo Okano ,  Tatsuya Shimizu

IPC分类号： G01N21/31 ,  G06F19/00 ,  G01N21/80 ,  G01N31/22 ,  C12M1/34

CPC分类号： G01N21/80 ,  C12M41/26 ,  G01N31/221 ,  G01N2021/3148 ,  G01N2021/3181 ,  G01N2201/0627

摘要： A pH measuring method includes: illuminating a medium solution, which includes: a first material; and a second material, with a plurality of light beams, which includes: a first light beam, a wavelength of which corresponds to a first absorption peak; a second light beam, a wavelength of which corresponds to a second absorption peak or a second convergence point; a third light beam, a wavelength of which corresponds to a third absorption peak or a third convergence point; and a fourth light beam, a wavelength of the fourth light beam at which an absorbance of at least one of the first and second materials is converged irrespective of a pH; receiving transmitted or reflected light beams of the first to fourth light beams; measuring absorbances at the wavelengths of the received light beams respectively; and calculating a pH of the medium solution based on the absorbances.

摘要翻译： pH测量方法包括：照射介质溶液，其包括：第一材料; 以及具有多个光束的第二材料，其包括：第一光束，其波长对应于第一吸收峰; 第二光束，其波长对应于第二吸收峰或第二会聚点; 第三光束，其波长对应于第三吸收峰或第三会聚点; 以及第四光束，所述第四光束的波长，其中所述第一和第二材料中的至少一个的吸光度在不同pH下会聚; 接收第一至第四光束的透射或反射光束; 分别测量接收光束波长处的吸光度; 并基于吸光度计算介质溶液的pH。

公开(公告)号：US09304088B2

公开(公告)日：2016-04-05

申请号：US13400106

申请日：2012-02-19

申请人： Mitsuhiro Oura ,  Teruo Okano ,  Tatsuya Shimizu ,  Hirotsugu Kubo ,  Sunao Takeda ,  Shinji Yamamori

发明人： Mitsuhiro Oura ,  Teruo Okano ,  Tatsuya Shimizu ,  Hirotsugu Kubo ,  Sunao Takeda ,  Shinji Yamamori

IPC分类号： G01N21/80 ,  G01N31/22 ,  G01N21/31 ,  C12M1/34

CPC分类号： G01N21/80 ,  C12M41/26 ,  G01N21/3151 ,  G01N31/221 ,  G01N2201/0627 ,  G01N2201/1211

摘要： A method of measuring a pH of a solution includes: emitting light beams of two wavelengths from one side of a measuring region of a solution into which an indicator is mixed, while pulsating the solution in the measuring region; receiving at least one of transmitted light beams and reflected light beams of the emitted light beams on the other side of the measuring region, while pulsating the solution in the measuring region; obtaining absorbances of the two wavelengths based on the received at least one of the transmitted light beams and the reflected light beams; obtaining an absorbance ratio from the obtained absorbances; and calculating a pH value of the solution based on the obtained absorbance ratio and an absorbance ratio/pH value correspondence database which is previously stored.

摘要翻译： 测量溶液的pH的方法包括：在测量区域中的溶液脉动的同时，从混合有指示剂的溶液的测量区域的一侧发射两个波长的光束; 在所述测量区域中的所述溶液脉动的同时，在所述测量区域的另一侧上接收所发射的光束的透射光束和反射光束中的至少一个; 基于所接收的所述透射光束和所述反射光束中的至少一个，获得所述两个波长的吸光度; 从所获得的吸光度获得吸光度比; 并且基于所获得的吸光度比和预先存储的吸光度比/ pH值对应数据库来计算溶液的pH值。

公开(公告)号：US09783773B2

公开(公告)日：2017-10-10

申请号：US13432658

申请日：2012-03-28

申请人： Mitsuhiro Oura ,  Teruo Okano ,  Tatsuya Shimizu ,  Hirotsugu Kubo ,  Sunao Takeda

发明人： Mitsuhiro Oura ,  Teruo Okano ,  Tatsuya Shimizu ,  Hirotsugu Kubo ,  Sunao Takeda

IPC分类号： C12M1/32 ,  C12M1/00

CPC分类号： C12M33/06 ,  C12M29/26 ,  C12M41/14 ,  C12M41/48

摘要： A cell culture apparatus includes: an isolator in which a sterile space accommodating a cell incubator filled with a culture solution containing cells to be cultured is disposed; a sampling unit configured to sample the culture solution in the cell incubator; a delivery flow path through which an inside of the sterile space and an outside of the sterile space communicate with each other, the delivery flow path configured to limit a flow in the delivery flow path to a direction that is directed from the inside of the sterile space toward the outside of the sterile space; and a culture solution delivering section configured to deliver the sampled culture solution to the outside of the sterile space via the delivery flow path.

公开(公告)号：US20120214250A1

公开(公告)日：2012-08-23

申请号：US13400106

申请日：2012-02-19

申请人： Mitsuhiro Oura ,  Teruo Okano ,  Tatsuya Shimizu ,  Hirotsugu Kubo ,  Sunao Takeda ,  Shinji Yamamori

发明人： Mitsuhiro Oura ,  Teruo Okano ,  Tatsuya Shimizu ,  Hirotsugu Kubo ,  Sunao Takeda ,  Shinji Yamamori

IPC分类号： G01N21/59

CPC分类号： G01N21/80 ,  C12M41/26 ,  G01N21/3151 ,  G01N31/221 ,  G01N2201/0627 ,  G01N2201/1211

摘要： A method of measuring a pH of a solution includes: emitting light beams of two wavelengths from one side of a measuring region of a solution into which an indicator is mixed, while pulsating the solution in the measuring region; receiving at least one of transmitted light beams and reflected light beams of the emitted light beams on the other side of the measuring region, while pulsating the solution in the measuring region; obtaining absorbances of the two wavelengths based on the received at least one of the transmitted light beams and the reflected light beams; obtaining an absorbance ratio from the obtained absorbances ; and calculating a pH value of the solution based on the obtained absorbance ratio and an absorbance ratio/pH value correspondence database which is previously stored.

摘要翻译： 测量溶液的pH的方法包括：在测量区域中的溶液脉动的同时，从混合有指示剂的溶液的测量区域的一侧发射两个波长的光束; 在所述测量区域中的所述溶液脉动的同时，在测量区域的另一侧上接收发射光束的透射光束和反射光束中的至少一个; 基于所接收的所述透射光束和所述反射光束中的至少一个，获得所述两个波长的吸光度; 从所获得的吸光度获得吸光度比; 并且基于所获得的吸光度比和预先存储的吸光度比/ pH值对应数据库来计算溶液的pH值。