公开(公告)号：US20240318132A1

公开(公告)日：2024-09-26

申请号：US18733979

申请日：2024-06-05

申请人： Miyaka Ogihara ,  Natsuko Hemmi ,  Naoki Satoh ,  Hidekazu Yaginuma ,  Masayuki Yumoto ,  Toshihiko Hosoya

发明人： Miyaka Ogihara ,  Natsuko Hemmi ,  Naoki Satoh ,  Hidekazu Yaginuma ,  Masayuki Yumoto ,  Toshihiko Hosoya

IPC分类号： C12N5/00 ,  B33Y70/10 ,  C08J3/075 ,  C12N5/0793

CPC分类号： C12N5/0062 ,  B33Y70/10 ,  C08J3/075 ,  C12N5/0619 ,  C08J2389/00

摘要： An object of the present invention is to provide a hydrogel that has both gel shapeability and cell adhesiveness, and enables a long-time cell culture. Provided is a hydrogel (1) including: a tetra-armed polymer (2) containing polyethylene glycol as a backbone, and containing one or more electrophilic functional groups or nucleophilic functional groups in at least one of a side chain and a terminal; and a linear polymer (3) containing two or more nucleophilic functional groups or electrophilic functional groups in at least one of a side chain and a terminal; the tetra-armed polymer (2) and the linear polymer (3) being cross-linked with each other; wherein the hydrogel is characterized in that the linear polymer (3) is protein, peptide, or polysaccharide, that the electrophilic functional group(s) is/are one or more selected from the group consisting of maleimidyl, N-hydroxy-succinimidyl (NHS), sulfosuccinimidyl, phthalimidyl, imidazoyl, acryloyl, and nitrophenyl, and that the nucleophilic functional group(s) is/are one or more selected from the group consisting of thiol, amino, and —CO2PhNO2.