公开(公告)号：US20230203086A1

公开(公告)日：2023-06-29

申请号：US17854187

申请日：2022-06-30

Applicant: ModernaTX, Inc.

Inventor： William Joseph ISSA ,  John Grant AUNINS ,  Stephane BANCEL

IPC: C07H21/02 ,  C12Q1/6806 ,  C12N15/10 ,  C07H1/06 ,  C08B37/00

CPC classification number: C07H21/02 ,  C07H1/06 ,  C08B37/0039 ,  C12N15/1006 ,  C12Q1/6806

Abstract: Disclosed herein are methods for purifying RNA comprising poly A. Also disclosed herein are compositions such as surfaces and oligonucleotides for purifying RNA comprising polyA. Other embodiments are also disclosed. Commercially-available resins having polythymidine oligonucleotide ligands typically contain less than 30 thymidine (2′deoxy) residues and some commercial resin suppliers utilize a distribution of dT chain lengths, not of a discreet length.

公开(公告)号：US20190085368A1

公开(公告)日：2019-03-21

申请号：US16144282

申请日：2018-09-27

Applicant: ModernaTX, Inc.

Inventor： Stephane BANCEL ,  William Joseph ISSA ,  John Grant AUNINS ,  Tirtha CHAKRABORTY

IPC: C12P19/34 ,  C12N15/10 ,  C12Q1/6865

Abstract: Described are methods for production of RNA transcripts using a non-amplified, linearized DNA tempate in an in vitro transcription reaction. Enzymatic 5′ capping and oligo dT purification can also be included in the methods.