公开(公告)号：US20240229109A1

公开(公告)日：2024-07-11

申请号：US18284938

申请日：2022-03-31

Applicant: ModernaTX, Inc.

Inventor： Amy E. Rabideau ,  David Reid ,  Huijuan Li ,  Kristian Link ,  Tao Jiang ,  Eva-Maria Schneeberger

IPC: C12Q1/6825 ,  C12Q1/6806 ,  C12Q1/6876

CPC classification number: C12Q1/6825 ,  C12Q1/6876 ,  C12Q1/6806 ,  C12Q2600/16

Abstract: Aspects of the disclosure relate to methods for analyzing compositions comprising RNA species with unique nucleotide sequences for identification and/or ratio determination of RNAs. The disclosure is based, in part, on methods of cleaving identifying sequences from RNAs in a composition, and detecting the abundance of each identifying sequence to quantify the abundance of corresponding RNA species. Other aspects relate to methods for producing compositions comprising more than two RNA species, such as multivalent RNA compositions. The disclosure is based, in part, on methods of determining the proper amount of input DNA for in vitro transcription (IVT) reactions that will result in RNA being transcribed in a predetermined ratio. In some aspects, the disclosure relates to pharmaceutical compositions comprising multivalent RNA compositions produced by methods described, by the disclosure.