公开(公告)号：US20180362943A1

公开(公告)日：2018-12-20

申请号：US15766662

申请日：2016-10-07

Applicant: Monsanto Technology LLC

Inventor： Jaishree M. Chittoor ,  Ervin Nagy ,  Keith H. Turner

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/90 ,  C12N15/82

Abstract: Provided herein are systems, methods, and compositions for the modification of target DNA sequences. More particularly, systems, methods, and compositions for cleaving a target DNA in eukaryotic cells with a guide RNA capable of hybridizing with a target sequence and an RNA-guided DNA nuclease are provided. Also provided are vectors and vector systems which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods for identifying and validating novel CRISPR systems.

公开(公告)号：US20200308632A1

公开(公告)日：2020-10-01

申请号：US16642339

申请日：2018-08-29

Applicant: Monsanto Technology LLC

Inventor： Adil Anjem ,  Amy L. Caruano-Yzermans ,  Todd A. Ciche ,  Julia L. Stevens ,  Keith H. Turner

IPC: C12Q1/689 ,  C12Q1/18 ,  A01N63/27 ,  A01N63/23 ,  A01N63/36

Abstract: Methods useful in detecting viable microbes in an agricultural composition are provided. Certain embodiments relate to methods in which a sample of an agricultural composition is obtained, and an amount of at least one pre-rRNA from at least one microbe is detected. The detected pre-rRNA from an agricultural sample may be compared with the amount of pre-rRNA from a control sample to determine the presence of a viable microbe.

公开(公告)号：US11692182B2

公开(公告)日：2023-07-04

申请号：US15766662

申请日：2016-10-07

Applicant: Monsanto Technology LLC

Inventor： Jaishree Chittoor ,  Ervin Nagy ,  Keith H. Turner

IPC: C12N15/82 ,  C12N9/22 ,  C12N5/04 ,  C12N15/10 ,  C12N15/90 ,  C12N15/63 ,  C12N15/11

CPC classification number: C12N9/22 ,  C12N5/04 ,  C12N15/102 ,  C12N15/11 ,  C12N15/111 ,  C12N15/63 ,  C12N15/8213 ,  C12N15/90 ,  C12N15/902 ,  C07K2319/09 ,  C07K2319/60 ,  C12N2310/20 ,  C12N2510/00 ,  C12N2800/80

Abstract: Provided herein are systems, methods, and compositions for the modification of target DNA sequences. More particularly, systems, methods, and compositions for cleaving a target DNA in eukaryotic cells with a guide RNA capable of hybridizing with a target sequence and an RNA-guided DNA nuclease are provided. Also provided are vectors and vector systems which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods for identifying and validating novel CRISPR systems.