公开(公告)号：US11414669B2

公开(公告)日：2022-08-16

申请号：US16563581

申请日：2019-09-06

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Stanislaw Flasinski ,  Elysia Krieger ,  Ervin Nagy ,  Krishnakumar Sridharan ,  Xudong Ye

IPC: C12N15/82

Abstract: This disclosure is related to plant-optimized recombinant nucleic acids encoding Cpf1 and their use in planta. Also disclosed are compositions, expression cassettes, and plant cells comprising the recombinant nucleic acids as well as methods and kits for modifying a target sequence in a plant genome using the recombinant nucleic acids.

公开(公告)号：US10995327B2

公开(公告)日：2021-05-04

申请号：US16066996

申请日：2016-12-29

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Jaishree M. Chittoor ,  Ervin Nagy

IPC: C12N9/22 ,  C12N15/82 ,  C12N15/10 ,  C12N15/11

Abstract: Provided herein are systems, methods, and compositions for the modification of target DNA sequences. More particularly, systems, methods, and compositions for editing genomic DNA in eukaryotic cells with a CRISPR-associated transposase are provided. Also provided are vectors and vector systems which encode one or more CRISPR-associated transposases, as well as methods for the design and use of such vectors. Also provided are methods for identifying and validating novel CRISPR-associated transposases.

公开(公告)号：US20180362943A1

公开(公告)日：2018-12-20

申请号：US15766662

申请日：2016-10-07

Applicant: Monsanto Technology LLC

Inventor： Jaishree M. Chittoor ,  Ervin Nagy ,  Keith H. Turner

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/90 ,  C12N15/82

Abstract: Provided herein are systems, methods, and compositions for the modification of target DNA sequences. More particularly, systems, methods, and compositions for cleaving a target DNA in eukaryotic cells with a guide RNA capable of hybridizing with a target sequence and an RNA-guided DNA nuclease are provided. Also provided are vectors and vector systems which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods for identifying and validating novel CRISPR systems.

公开(公告)号：US12157893B2

公开(公告)日：2024-12-03

申请号：US17523755

申请日：2021-11-10

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： John Bradley ,  Larry A. Gilbertson ,  Ervin Nagy ,  Christine Shyu ,  Peizhen Yang ,  Xudong Ye

IPC: C12N15/82

Abstract: The present disclosure relates to compositions and methods for improving site-directed integration frequency in the genome of plant cells.

公开(公告)号：US11859191B2

公开(公告)日：2024-01-02

申请号：US17817196

申请日：2022-08-03

Applicant: Monsanto Technology LLC

Inventor： Stanislaw Flasinski ,  Elysia Krieger ,  Ervin Nagy ,  Krishnakumar Sridharan ,  Xudong Ye

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C12N15/8205 ,  C12N2310/20 ,  C12N2800/80

Abstract: This disclosure is related to plant-optimized recombinant nucleic acids encoding Cpf1 and their use in planta. Also disclosed are compositions, expression cassettes, and plant cells comprising the recombinant nucleic acids as well as methods and kits for modifying a target sequence in a plant genome using the recombinant nucleic acids.

公开(公告)号：US12275944B2

公开(公告)日：2025-04-15

申请号：US18501949

申请日：2023-11-03

Applicant: Monsanto Technology LLC

Inventor： Stanislaw Flasinski ,  Elysia Krieger ,  Ervin Nagy ,  Krishnakumar Sridharan ,  Xudong Ye

IPC: C12N15/82

Abstract: This disclosure is related to plant-optimized recombinant nucleic acids encoding Cpf1 and their use in planta. Also disclosed are compositions, expression cassettes, and plant cells comprising the recombinant nucleic acids as well as methods and kits for modifying a target sequence in a plant genome using the recombinant nucleic acids.

公开(公告)号：US12006521B2

公开(公告)日：2024-06-11

申请号：US17226264

申请日：2021-04-09

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Jaishree M. Chittoor ,  Ervin Nagy

IPC: C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  C12N15/82

CPC classification number: C12N9/22 ,  C12N15/102 ,  C12N15/11 ,  C12N15/8241 ,  C12N2310/20 ,  C12N2800/80

Abstract: Provided herein are systems, methods, and compositions for the modification of target DNA sequences. More particularly, systems, methods, and compositions for editing genomic DNA in eukaryotic cells with a CRISPR-associated transposase are provided. Also provided are vectors and vector systems which encode one or more CRISPR-associated transposases, as well as methods for the design and use of such vectors. Also provided are methods for identifying and validating novel CRISPR-associated transposases.

公开(公告)号：US11692182B2

公开(公告)日：2023-07-04

申请号：US15766662

申请日：2016-10-07

Applicant: Monsanto Technology LLC

Inventor： Jaishree Chittoor ,  Ervin Nagy ,  Keith H. Turner

IPC: C12N15/82 ,  C12N9/22 ,  C12N5/04 ,  C12N15/10 ,  C12N15/90 ,  C12N15/63 ,  C12N15/11

CPC classification number: C12N9/22 ,  C12N5/04 ,  C12N15/102 ,  C12N15/11 ,  C12N15/111 ,  C12N15/63 ,  C12N15/8213 ,  C12N15/90 ,  C12N15/902 ,  C07K2319/09 ,  C07K2319/60 ,  C12N2310/20 ,  C12N2510/00 ,  C12N2800/80

Abstract: Provided herein are systems, methods, and compositions for the modification of target DNA sequences. More particularly, systems, methods, and compositions for cleaving a target DNA in eukaryotic cells with a guide RNA capable of hybridizing with a target sequence and an RNA-guided DNA nuclease are provided. Also provided are vectors and vector systems which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods for identifying and validating novel CRISPR systems.

公开(公告)号：US20210147862A1

公开(公告)日：2021-05-20

申请号：US17078919

申请日：2020-10-23

Applicant: Pairwise Plants Services, Inc. ,  Monsanto Technology LLC

Inventor： Aaron Hummel ,  Joseph Matthew Watts ,  Shai Joshua Lawit ,  Nathaniel Graham ,  Larry Gilbertson ,  Ervin Nagy ,  Linda Rymarquis

IPC: C12N15/82 ,  C12N15/113 ,  C12N9/22

Abstract: This invention relates to recombinant nucleic constructs comprising a DNA binding domain, an endonuclease and a reverse transcriptase and methods of use thereof for modifying nucleic acids in plants.