公开(公告)号：US20220119826A1

公开(公告)日：2022-04-21

申请号：US17520352

申请日：2021-11-05

Applicant: Monsanto Technology LLC

Inventor： Beth Jo Calabotta ,  Kevin Lee Depperman ,  Erik D. Dersch ,  Jerald D. Heise ,  Angela Ranae Koestel ,  Cindy L. Ludwig ,  Brian J. Martinell ,  Edward Williams

IPC: C12N15/82 ,  C12N5/00 ,  A01H4/00 ,  C12N5/04 ,  G01N33/00 ,  C12N9/10

Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

公开(公告)号：US20210254084A1

公开(公告)日：2021-08-19

申请号：US17124115

申请日：2020-12-16

Applicant: Monsanto Technology LLC

Inventor： Beth Jo Calabotta ,  Kevin Lee Depperman ,  Erik D. Dersch ,  Jerald D. Heise ,  Angela Ranae Koestel ,  Cindy L. Ludwig ,  Brian J. Martinell ,  Edward Williams

IPC: C12N15/82 ,  C12N5/00 ,  A01H4/00 ,  C12N5/04 ,  G01N33/00 ,  C12N9/10

Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.