公开(公告)号：US20250110152A1

公开(公告)日：2025-04-03

申请号：US18844070

申请日：2023-08-31

Applicant: NANJING FORESTRY UNIVERSITY

Inventor： Caoxing HUANG ,  Xiaoxue ZHAO ,  Qiang YONG ,  Chenhuan LAI ,  Yongcan JIN

IPC: G01Q60/42 ,  C12Q1/40 ,  G01Q60/28

Abstract: A method for characterizing the interaction force between lignin and cellulase is provided. The method includes the following steps: (1) preparation of lignin film: preparing a lignin solution, then dropwise adding the solution onto a silicon wafer, repeatedly spin-coating with a spin coater, and vacuum-drying the prepared lignin film; (2) AFM probe modification: modifying an AFM probe coated with a gold film on the surface with HS-PEG-COOH and then activating the carboxyl group, immersing the AFM probe after the carboxyl group activation in a PBS solution of cellulase for incubation, and after the modification is completed, placing the washed AFM probe in a PBS buffer solution for storage for later use; (3) testing the lignin-cellulase interaction force by using an AFM instrument in a liquid phase environment. AFM is used to quantitatively characterize the interaction between the cellulase and lignin in a liquid environment.

公开(公告)号：US20240035056A1

公开(公告)日：2024-02-01

申请号：US18274770

申请日：2021-05-06

Applicant: NANJING FORESTRY UNIVERSITY

Inventor： Caoxing HUANG ,  Qiang YONG ,  Yuheng TAO ,  Chenhuan LAI ,  Yong XU ,  Xin LI

IPC: C12P19/04 ,  C12N9/40 ,  C12N9/24

CPC classification number: C12P19/04 ,  C12N9/2465 ,  C12Y302/01022 ,  C12N9/2491 ,  C12Y302/01025

Abstract: A method and special complex enzyme for hydrolyzing a galactomannan (GM) to prepare a small-molecule GM and a galactomannan oligosaccharide (GMOS) is provided. The method includes: conducting fermentation with microcrystalline cellulose (MCC) and melibiose as carbon sources and Trichoderma reesei (T. reesei) as an enzyme-producing strain to obtain a supernatant, which is a complex enzyme solution with enzymatic activities of β-mannanase and α-galactosidase; and directly using the complex enzyme solution for enzymatic hydrolysis of a GM as a substrate to prepare the small-molecule GM and the GMOS.