公开(公告)号：US20240035056A1

公开(公告)日：2024-02-01

申请号：US18274770

申请日：2021-05-06

Applicant: NANJING FORESTRY UNIVERSITY

Inventor： Caoxing HUANG ,  Qiang YONG ,  Yuheng TAO ,  Chenhuan LAI ,  Yong XU ,  Xin LI

IPC: C12P19/04 ,  C12N9/40 ,  C12N9/24

CPC classification number: C12P19/04 ,  C12N9/2465 ,  C12Y302/01022 ,  C12N9/2491 ,  C12Y302/01025

Abstract: A method and special complex enzyme for hydrolyzing a galactomannan (GM) to prepare a small-molecule GM and a galactomannan oligosaccharide (GMOS) is provided. The method includes: conducting fermentation with microcrystalline cellulose (MCC) and melibiose as carbon sources and Trichoderma reesei (T. reesei) as an enzyme-producing strain to obtain a supernatant, which is a complex enzyme solution with enzymatic activities of β-mannanase and α-galactosidase; and directly using the complex enzyme solution for enzymatic hydrolysis of a GM as a substrate to prepare the small-molecule GM and the GMOS.