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    CONSTRUCTION OF MICRORNA GENE-MEDIATED NOVEL TISSUE ENGINEERED NERVE AND APPLICATIONS THEREOF IN REPAIRING NERVE DEFECT
    2.
    发明申请
    CONSTRUCTION OF MICRORNA GENE-MEDIATED NOVEL TISSUE ENGINEERED NERVE AND APPLICATIONS THEREOF IN REPAIRING NERVE DEFECT 审中-公开

    公开(公告)号：US20190111179A1

    公开(公告)日：2019-04-18

    申请号：US16089190

    申请日：2017-04-05

    申请人： NANTONG UNIVERSITY

    发明人： Xiaosong GU ,  Fei DING ,  Xin TANG ,  Yumin YANG ,  Bin YU ,  Shiying LI ,  Songlin ZHOU ,  Luzhong ZHANG ,  Yaxian WANG ,  Yun GU ,  Hualin SUN

    IPC分类号： A61L27/36 ,  A61L27/54 ,  A61L27/56

    摘要： Provided is a use of one or more MicroRNA genes selected from miRNAs of Family Let-7, miR-21 or miR-222 in the construction of tissue engineered nerves and in the repair of peripheral nerve defects. An outer and/or internal surface or pores of a tissue engineered nerve graft are coated or adsorbed with polymeric nanomicrospheres carrying a Let-7 family miRNA inhibitor, miR-21, or miR-222, or a mimetic thereof, wherein the polymeric material is composed of biocompatible fibronectin and heparin. The regeneration of peripheral nerves and the construction of tissue engineered nerves are promoted by regulating the expression of MicroRNA genes which can effectively promote the proliferation of primary Schwann cells cultured in vitro and have an anti-apoptotic effect on neuronal cells. In-vivo test proves that bridging of the tissue engineered nerve graft can facilitate the regeneration of peripheral nerves, thus being useful in the treatment of peripheral nerve injury.

    FRUCTUS FORSYTHIAE AND RADIX ASTRAGALI COMPOUND PREPARATION, AND PREPARATION METHOD THEREFOR AND USE THEREOF
    3.
    发明申请
    FRUCTUS FORSYTHIAE AND RADIX ASTRAGALI COMPOUND PREPARATION, AND PREPARATION METHOD THEREFOR AND USE THEREOF 有权

    公开(公告)号：US20230040479A1

    公开(公告)日：2023-02-09

    申请号：US17788010

    申请日：2020-07-14

    申请人： NANTONG UNIVERSITY

    发明人： Xiaosong GU ,  Chunkang TANG ,  Leilei GONG ,  Yu ZHANG ,  Qiong CHENG ,  Xiaoming YANG ,  Xiaomin WANG

    IPC分类号： A61K36/634 ,  A61K36/481 ,  A61K31/235 ,  A61K9/16 ,  A61P31/16

    摘要： A Fructus Forsythiae and Radix Astragali compound preparation, and a preparation method therefor and a use thereof is provided. Traditional Chinese medicine formulation components consist of the following raw materials or raw material extracts in parts by mass: 9-11 parts of Flos Lonicerae, 9-11 parts of Fructus Forsythiae, 9-11 parts of Radix Scutellariae, 9-11 parts of Herba Artemisiae Annuae, 9-11 parts of Radix Astragali, 9-11 parts of stir-fried Rhizoma Atractylodis Macrocephalae, 9-11 parts of Herba Pogostemonis, 5-7 parts of Radix Saposhnikoviae, 9-11 parts of Radix Ophiopogonis, and 5-7 parts of Radix Glycyrrhizae. The components can be made into an oral liquid, a granule, a dissolved medicine, or a tablet. Further disclosed is a use of the Fructus Forsythiae and Radix Astragali compound preparation in preparation of medicines for preventing and/or treating a viral influenza disease.

    CONSTRUCTION AND APPLICATION OF DIFFERENTIALLY REGULATED TISSUE-ENGINEERED NERVE GRAFTS
    4.
    发明申请
    CONSTRUCTION AND APPLICATION OF DIFFERENTIALLY REGULATED TISSUE-ENGINEERED NERVE GRAFTS 有权

    公开(公告)号：US20210268149A1

    公开(公告)日：2021-09-02

    申请号：US17044837

    申请日：2019-09-11

    申请人： NANTONG UNIVERSITY

    发明人： Xin TANG ,  Cheng SUN ,  Gang CHEN ,  Xiaosong GU ,  Yumin YANG ,  Fei DING ,  Yanpei GONG ,  Qianru HE ,  Hongkui WANG ,  Mi SHEN

    IPC分类号： A61L27/36 ,  A61L27/38 ,  A61L27/54 ,  A61L27/56

    摘要： A differential tissue-engineered nerve including motor-like nerves and sensory-like nerves. The motor-like nerve and the sensory-like nerve respectively includes a motor-like nerve outer tube and a motor-like nerve fiber in the outer tube as well as a sensory-like nerve outer tube and a sensory-like nerve fiber in the outer tube. Schwann cells and/or fibroblasts derived from motor nerves and sensory nerves are respectively contained in surfaces or pores of the motor-like and sensory-like nerve outer tubes. Transsynaptic signal molecules Neuroligin-1 and Neuroligin-2 are contained in surfaces or pores of the motor-like and sensory-like nerve fibers. Neuroligin-1 is selectively used to specifically promote synaptic remodeling of motor neurons, while Neuroligin-2 is selectively used to specifically promote synaptic remodeling of sensory neurons, so that repair efficiency of motor nerve cells and sensory nerve cells is improved.

    PREPARATION METHOD FOR SILK FIBROIN NERVE GRAFT FUSED WITH NT3
    8.
    发明公开
    PREPARATION METHOD FOR SILK FIBROIN NERVE GRAFT FUSED WITH NT3 审中-公开

    公开(公告)号：US20230144643A1

    公开(公告)日：2023-05-11

    申请号：US17633950

    申请日：2020-07-24

    申请人： NANTONG UNIVERSITY

    发明人： Yan LIU ,  Mei LIU ,  Xiaosong GU ,  Tuchen GUAN

    IPC分类号： A61L27/22

    CPC分类号： A61L27/227 ,  A61L2430/32

    摘要： A silk fibroin nerve graft fused with NT3 and a preparation method therefor is provided. The method includes the steps of: synthesizing a gene fragment containing a silk fibroin light chain and NT-3, connecting the fragment to a pET-30 expression vector, and transferring the obtained recombinant expression vector into BL21 Escherichia coli to obtain a fusion protein; placing a silk fibroin fiber web in a mold, mixing the fusion protein and a silk fibroin solution, and performing freeze drying to enable the silk fibroin to be crosslinked with the fusion protein to form a nerve conduit; and after deformation processing, finally obtaining a silk fibroin nerve graft having NT-3 activity. The silk fibroin nerve graft can provide a mechanical support, exert nerve protection and nerve regeneration promotion functions in a long term, and adjust the proportion of NT-3 bioactive peptides in the nerve conduit, facilitating repair of nerve injuries.