Cell lines and virus culture
    1.
    发明授权
    Cell lines and virus culture 失效
    细胞系和病毒培养

    公开(公告)号:US3871954A

    公开(公告)日:1975-03-18

    申请号:US43091874

    申请日:1974-01-04

    Applicant: NAT RES DEV

    CPC classification number: C12N7/00 C12N5/067 C12N2770/32364

    Abstract: A method for producing a human embryo liver cell line which comprises disaggregating human embryo liver, suspending the cells in a growth medium, replacing this with fresh medium if it becomes acid, and allowing a confluent sheet of spindle-shaped cells of the cell line to form. The cells of the cell line, which are designated by A.T.C.C. No. CL99, may be sub-cultured and may be used as the basis of a cell-culture system for the culturing of mammalian cells in tissue culture. Viruses may be cultivated in a culture system based on cells of the cell line to provide antigenic material for the preparation of vaccines.

    Abstract translation: 一种生产人胚胎肝细胞系的方法,其包括将人胚肝分解,将细胞悬浮于生长培养基中,如果其变为酸,则用新鲜培养基代替该胚细胞,并将细胞系的纺锤形细胞的汇合片 形成。 细胞系的细胞,由A.T.C.C. 编号CL99可以被亚培养,并且可以用作组织培养中哺乳动物细胞培养的细胞培养系统的基础。 可以在基于细胞系的细胞的培养系统中培养病毒,以提供用于疫苗制备的抗原材料。

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