公开(公告)号：US3871954A

公开(公告)日：1975-03-18

申请号：US43091874

申请日：1974-01-04

Applicant: NAT RES DEV

Inventor： ZUCKERMAN ARIE JEREMY

IPC: C12N5/071 ,  C12N7/08 ,  C12K9/00

CPC classification number: C12N7/00 ,  C12N5/067 ,  C12N2770/32364

Abstract: A method for producing a human embryo liver cell line which comprises disaggregating human embryo liver, suspending the cells in a growth medium, replacing this with fresh medium if it becomes acid, and allowing a confluent sheet of spindle-shaped cells of the cell line to form. The cells of the cell line, which are designated by A.T.C.C. No. CL99, may be sub-cultured and may be used as the basis of a cell-culture system for the culturing of mammalian cells in tissue culture. Viruses may be cultivated in a culture system based on cells of the cell line to provide antigenic material for the preparation of vaccines.

Abstract translation: 一种生产人胚胎肝细胞系的方法，其包括将人胚肝分解，将细胞悬浮于生长培养基中，如果其变为酸，则用新鲜培养基代替该胚细胞，并将细胞系的纺锤形细胞的汇合片 形成。 细胞系的细胞，由A.T.C.C. 编号CL99可以被亚培养，并且可以用作组织培养中哺乳动物细胞培养的细胞培养系统的基础。 可以在基于细胞系的细胞的培养系统中培养病毒，以提供用于疫苗制备的抗原材料。