公开(公告)号：US11136612B2

公开(公告)日：2021-10-05

申请号：US16325847

申请日：2017-08-07

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Hitoshi Muguruma ,  Atsunori Hiratsuka ,  Hisanori Iwasa ,  Jun Takagi ,  Hiroyasu Kadoya

IPC: G01N27/327 ,  C12Q1/00 ,  C12M1/40 ,  G01N27/416 ,  C12Q1/32 ,  C01B32/158 ,  C12N9/04 ,  B82Y30/00 ,  B82Y40/00

Abstract: A reagent used for a glucose sensor for electrochemical, quantitative determination of glucose, includes a flavin adenine dinucleotide glucose dehydrogenase, single-walled carbon nanotubes, and a dispersant.

公开(公告)号：US20150267178A1

公开(公告)日：2015-09-24

申请号：US14425837

申请日：2013-09-09

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Kazumichi Ozawa ,  Noriko Sasaki ,  Atsunori Hiratsuka ,  Kenji Yokoyama ,  Nao Kinoshita ,  Hideaki Ooe ,  Jun Takagi ,  Koji Tanaka ,  Katsushige Kotani

IPC: C12N9/04 ,  C12Q1/54

CPC classification number: C12N9/0006 ,  C12Q1/006 ,  C12Q1/54 ,  C12Y101/9901 ,  G01N2333/904 ,  H01M4/90 ,  H01M8/16 ,  Y02E60/527

Abstract: The present invention provides a protein derived from a thermophilic filamentous fungus having flavin adenine dinucleotide-dependent glucose dehydrogenase activity. The present invention provides an enzyme for glucose measurement that has better thermal stability than that of conventional enzymes while being unaffected by dissolved oxygen, not requiring the addition of a coenzyme, and maintaining the excellent properties of FADGDH in terms of having excellent substrate specificity (particularly low reactivity with maltose).

Abstract translation: 本发明提供衍生自具有黄素腺嘌呤二核苷酸依赖性葡萄糖脱氢酶活性的嗜热丝状真菌的蛋白质。 本发明提供了一种具有比常规酶更好的热稳定性的酶的葡萄糖测定酶，而不受溶解氧的影响，不需要加入辅酶，并且在具有优异的底物特异性（特别是，特别是）的情况下，保持了FADGDH的优异性能 低麦芽糖的反应性）。