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    METHOD AND SYSTEM FOR EXPRESSING FOREIGN GENE IN A PLANT
    1.
    发明申请
    METHOD AND SYSTEM FOR EXPRESSING FOREIGN GENE IN A PLANT 审中-公开

    公开(公告)号：US20190062767A1

    公开(公告)日：2019-02-28

    申请号：US15691507

    申请日：2017-08-30

    Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

    Inventor： Noriho Fukuzawa ,  Takeshi Matsumura

    IPC: C12N15/82

    Abstract: A method is provided for expressing a foreign gene, comprising the steps of: (a) producing a plant which functionally expresses an RNA1 genome and/or an RNA2 genome of Cucumber Mosaic Virus (CMV); (b) introducing a nucleic acid vector into the plant, said nucleic acid vector comprising, arranged in this order: (i) a right border sequence (RB) derived from the Agrobacterium T-DNA sequence; (ii) an expression promoter sequence workable in the plant; (iii) a sequence corresponding to an RNA3 genome of CMV in which a 3b gene, which codes for a 3b protein (coat protein), of CMV has been prevented from being expressed and the foreign gene has been inserted downstream of, and operably linked with, a subgenomic promoter which otherwise promotes the expression of the 3b gene; and (iv) a left border sequence (LB) derived from the Agrobacterium T-DNA sequence; and (c) culturing the plant containing the nucleic acid vector such that the RNA1 and/or RNA2 genome(s) of CMV is functionally expressed by the plant while the foreign gene is expressed transiently from the nucleic acid vector along with the CMV RNA3 genome lacking a region corresponding to the 3b gene.

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