公开(公告)号：US20240004176A1

公开(公告)日：2024-01-04

申请号：US18368621

申请日：2023-09-15

Applicant: NIKON CORPORATION

Inventor： Shota TSUCHIDA ,  Satoshi IKEDA

IPC: G02B21/00 ,  G01J9/00 ,  G01J9/02

CPC classification number: G02B21/0056 ,  G01J9/00 ,  G01J9/0246 ,  G01J2009/0211 ,  G01J2009/0269

Abstract: A quantitative phase image generating method for a microscope, includes: irradiating an object with illumination light; disposing a focal point of an objective lens at each of a plurality of positions that are mutually separated by gaps Δz along an optical axis of the objective lens, and detecting light from the object; generating sets of light intensity distribution data corresponding to each of the plurality of positions based upon the detected light; and generating a quantitative phase image based upon the light intensity distribution data; wherein the gap Δz is set based upon setting information of the microscope.

公开(公告)号：US20200271910A1

公开(公告)日：2020-08-27

申请号：US15931995

申请日：2020-05-14

Applicant: NIKON CORPORATION

Inventor： Shota TSUCHIDA ,  Satoshi IKEDA

IPC: G02B21/00 ,  G02B21/36

Abstract: A quantitative phase image generating method for a microscope, includes: irradiating an object with illumination light; disposing a focal point of an objective lens at each of a plurality of positions that are mutually separated by gaps Δz along an optical axis of the objective lens, and detecting light from the object; generating sets of light intensity distribution data corresponding to each of the plurality of positions based upon the detected light; and generating a quantitative phase image based upon the light intensity distribution data; wherein the gap Δz is set based upon setting information of the microscope.

公开(公告)号：US20190101738A1

公开(公告)日：2019-04-04

申请号：US16193214

申请日：2018-11-16

Applicant: NIKON CORPORATION

Inventor： Shota TSUCHIDA ,  Kei HORIE

IPC: G02B21/16 ,  G01N21/64 ,  G02B21/36 ,  G02B21/06

Abstract: It is aimed to prevent excitation light from leaking and being detected by the detector in a fluorescence microscope. A microscope includes an illumination optical system configured to illuminate a specimen with excitation light, a detector configured to detect fluorescence emitted from the specimen, and an observation optical system configured to guide the fluorescence to the detector. The observation optical system includes an optical filter having a variable wavelength characteristic, a light dispersing element configured to disperse, into light beams, light that has passed through the optical filter, and a light block plate configured to block at least some of the light beams resulting from the dispersion by the light dispersing element.

公开(公告)号：US20240003814A1

公开(公告)日：2024-01-04

申请号：US18369447

申请日：2023-09-18

Applicant: NIKON CORPORATION

Inventor： Shota TSUCHIDA ,  Yasunori MORISHITA ,  Yuta OSHIDARI

IPC: G01N21/64 ,  G02B21/00

CPC classification number: G01N21/6458 ,  G02B21/0076 ,  G01N2021/6471

Abstract: A microscope includes: an illumination optical system which irradiates a specimen with excitation light; a detector which detects fluorescence emitted from the specimen; and an observation optical system which guides fluorescence to the detector and includes: a first optical filter having wavelength reflection and transmission characteristics that vary depending on where light enters; a second optical filter disposed in an optical path of light reflected by the first optical filter, having a boundary wavelength of transmission changing with respect to a position along a first direction, and transmitting light having a wavelength longer than a first boundary wavelength at where the reflected light enters; and a third optical filter disposed in the optical path and having a boundary wavelength of transmission changing with respect to a position along the first direction, and transmitting light having a wavelength shorter than a second boundary wavelength at where the reflected light enters.