公开(公告)号：US12098215B2

公开(公告)日：2024-09-24

申请号：US16307621

申请日：2017-06-06

Applicant: NORTHWESTERN UNIVERSITY

Inventor： Milan Mrksich ,  Justin A. Modica

IPC: C07K16/46 ,  A61P19/02 ,  A61P35/00 ,  A61P37/00 ,  C07K16/32 ,  C12N9/18 ,  A61K39/00

CPC classification number: C07K16/46 ,  A61P19/02 ,  A61P35/00 ,  A61P37/00 ,  C07K16/32 ,  C12N9/18 ,  C12Y301/01074 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/92 ,  C07K2317/94 ,  C07K2319/00 ,  C07K2319/61 ,  C12Y201/01037 ,  C12Y301/21002 ,  C12Y302/01021 ,  C12Y304/11018 ,  C12Y304/24024 ,  C12Y305/02006

Abstract: The disclosure provides constructs comprising a first fusion protein, a second fusion protein, and a linker, wherein the first fusion protein and the second fusion protein each include an affinity reagent and a reactive enzyme, and the linker includes a first and second functional groups specific for irreversibly inhibiting the first and second fusion protein reactive enzymes. The disclosure further provides a method including (a) contacting a first fusion protein including an affinity reagent and a reactive enzyme with a linker including a functional group specific for irreversibly inhibiting the first fusion protein reactive enzyme thereby coupling the first fusion protein and the linker, and (b) contacting a second fusion protein including an affinity reagent and a reactive enzyme with the linker, the linker including a functional group specific for irreversibly inhibiting the second fusion protein reactive enzyme thereby coupling the second fusion protein and the linker.

公开(公告)号：US20190161556A1

公开(公告)日：2019-05-30

申请号：US16307621

申请日：2017-06-06

Applicant: NORTHWESTERN UNIVERSITY

Inventor： Milan Mrksich ,  Justin A. Modica

IPC: C07K16/46 ,  C07K16/32 ,  A61P35/00 ,  A61P19/02 ,  A61P37/00

Abstract: The disclosure provides constructs comprising a first fusion protein, a second fusion protein, and a linker, wherein the first fusion protein and the second fusion protein each include an affinity reagent and a reactive enzyme, and the linker includes a first and second functional groups specific for irreversibly inhibiting the first and second fusion protein reactive enzymes. The disclosure further provides a method including (a) contacting a first fusion protein including an affinity reagent and a reactive enzyme with a linker including a functional group specific for irreversibly inhibiting the first fusion protein reactive enzyme thereby coupling the first fusion protein and the linker, and (b) contacting a second fusion protein including an affinity reagent and a reactive enzyme with the linker, the linker including a functional group specific for irreversibly inhibiting the second fusion protein reactive enzyme thereby coupling the second fusion protein and the linker.

公开(公告)号：US20250059301A1

公开(公告)日：2025-02-20

申请号：US18805059

申请日：2024-08-14

Applicant: NORTHWESTERN UNIVERSITY

Inventor： Milan Mrksich ,  Justin A. Modica

IPC: C07K16/46 ,  A61K39/00 ,  A61P19/02 ,  A61P35/00 ,  A61P37/00 ,  C07K16/32 ,  C12N9/18

Abstract: The disclosure provides constructs comprising a first fusion protein, a second fusion protein, and a linker, wherein the first fusion protein and the second fusion protein each include an affinity reagent and a reactive enzyme, and the linker includes a first and second functional groups specific for irreversibly inhibiting the first and second fusion protein reactive enzymes. The disclosure further provides a method including (a) contacting a first fusion protein including an affinity reagent and a reactive enzyme with a linker including a functional group specific for irreversibly inhibiting the first fusion protein reactive enzyme thereby coupling the first fusion protein and the linker, and (b) contacting a second fusion protein including an affinity reagent and a reactive enzyme with the linker, the linker including a functional group specific for irreversibly inhibiting the second fusion protein reactive enzyme thereby coupling the second fusion protein and the linker.

公开(公告)号：US11530404B2

公开(公告)日：2022-12-20

申请号：US16306563

申请日：2017-05-25

Applicant: Northwestern University

Inventor： Omar K. Farha ,  Peng Li ,  Justin A. Modica ,  Milan Mrksich ,  Joseph T. Hupp

IPC: C12N11/02 ,  C12N11/14 ,  C12P9/00 ,  A62D3/02 ,  C12N9/16 ,  C12N9/18 ,  A62D101/02 ,  A62D101/26

Abstract: Enzyme-immobilizing MOFs and methods for their use in enzymatically catalyzed reactions are provided. The MOFs are channel-type MOFs that present a hierarchical pore structure comprising a first set of large channels sized for enzyme immobilization and a second set of smaller channels running alongside of the large channels that remain enzyme-free and allow for reactant delivery to the enzymes and product expulsion from the larger channels.