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    MICROFLUIDIC DEVICE FOR LIVE CELL MANIPULATION AND ANALYSIS
    1.
    发明申请
    MICROFLUIDIC DEVICE FOR LIVE CELL MANIPULATION AND ANALYSIS 有权

    公开(公告)号：US20220126292A1

    公开(公告)日：2022-04-28

    申请号：US17511246

    申请日：2021-10-26

    Applicant: NORTHWESTERN UNIVERSITY

    Inventor： Horacio Dante Espinosa ,  Milan Mrksich ,  Prithvijit Mukherjee ,  Eric Jason Berns ,  Cesar Andres Patino

    IPC: B01L3/00

    Abstract: A cell analysis system includes a multi-layer microfluidic device that includes a layer of microfluidic channels, a layer of microwells, a membrane with nanochannels, and a layer of extraction chambers. The microwells and the membrane are configured to allow culturing of cells that are adhered to the membrane or suspended in the microwells, and the membrane is configured to allow diffusion of substances across the membrane into the layer of extraction chambers. The cell analysis system includes a top conductive layer and a bottom conductive layer on the opposite sides of the multi-layer microfluidic device. The cell analysis system also includes a function generator configured to apply an electroporation pulse between the top conductive layer and the bottom conductive layer.

    ENZYME COUPLED ASSAY FOR QUANTIFICATION OF PROTEIN AND PEPTIDE BINDING BY SAMDI MASS SPECTROMETRY
    2.
    发明申请
    ENZYME COUPLED ASSAY FOR QUANTIFICATION OF PROTEIN AND PEPTIDE BINDING BY SAMDI MASS SPECTROMETRY 审中-公开
    通过SAMDI质谱法定量蛋白质和肽结合的酶联合测定

    公开(公告)号：US20160252501A1

    公开(公告)日：2016-09-01

    申请号：US15051338

    申请日：2016-02-23

    Applicant: NORTHWESTERN UNIVERSITY

    Inventor： Milan Mrksich ,  Patrick O'Kane

    IPC: G01N33/543

    CPC classification number: G01N33/54306 ,  G01N33/54353 ,  G01N2333/47 ,  G01N2333/98 ,  G01N2560/00

    Abstract: Disclosed herein are methods of using an immobilized substrate, immobilized ligand, and a fusion protein of an enzyme for the substrate and a receptor for the ligand, where the immobilized substrate can react to form an immobilized product that has a different mass than the immobilized substrate, and using this transformation to indirectly determine the binding of the receptor and the ligand. These methods can be used for high-throughput screening for possible modulators (e.g., inhibitors or activators) of the ligand-receptor interaction.

    Abstract translation: 本文公开了使用固定化底物，固定化配体和用于底物的酶的融合蛋白和配体受体的方法，其中固定化的底物可以反应以形成具有与固定化基质不同的质量的固定化产物 并且使用该转化间接测定受体和配体的结合。 这些方法可用于高通量筛选配体 - 受体相互作用的可能的调节剂（例如抑制剂或激活剂）。

    Fusion Protein Construct
    6.
    发明申请
    Fusion Protein Construct 审中-公开

    公开(公告)号：US20190161556A1

    公开(公告)日：2019-05-30

    申请号：US16307621

    申请日：2017-06-06

    Applicant: NORTHWESTERN UNIVERSITY

    Inventor： Milan Mrksich ,  Justin A. Modica

    IPC: C07K16/46 ,  C07K16/32 ,  A61P35/00 ,  A61P19/02 ,  A61P37/00

    Abstract: The disclosure provides constructs comprising a first fusion protein, a second fusion protein, and a linker, wherein the first fusion protein and the second fusion protein each include an affinity reagent and a reactive enzyme, and the linker includes a first and second functional groups specific for irreversibly inhibiting the first and second fusion protein reactive enzymes. The disclosure further provides a method including (a) contacting a first fusion protein including an affinity reagent and a reactive enzyme with a linker including a functional group specific for irreversibly inhibiting the first fusion protein reactive enzyme thereby coupling the first fusion protein and the linker, and (b) contacting a second fusion protein including an affinity reagent and a reactive enzyme with the linker, the linker including a functional group specific for irreversibly inhibiting the second fusion protein reactive enzyme thereby coupling the second fusion protein and the linker.

    Fusion Protein Construct
    7.
    发明申请
    Fusion Protein Construct 有权

    公开(公告)号：US20250059301A1

    公开(公告)日：2025-02-20

    申请号：US18805059

    申请日：2024-08-14

    Applicant: NORTHWESTERN UNIVERSITY

    Inventor： Milan Mrksich ,  Justin A. Modica

    IPC: C07K16/46 ,  A61K39/00 ,  A61P19/02 ,  A61P35/00 ,  A61P37/00 ,  C07K16/32 ,  C12N9/18

    Abstract: The disclosure provides constructs comprising a first fusion protein, a second fusion protein, and a linker, wherein the first fusion protein and the second fusion protein each include an affinity reagent and a reactive enzyme, and the linker includes a first and second functional groups specific for irreversibly inhibiting the first and second fusion protein reactive enzymes. The disclosure further provides a method including (a) contacting a first fusion protein including an affinity reagent and a reactive enzyme with a linker including a functional group specific for irreversibly inhibiting the first fusion protein reactive enzyme thereby coupling the first fusion protein and the linker, and (b) contacting a second fusion protein including an affinity reagent and a reactive enzyme with the linker, the linker including a functional group specific for irreversibly inhibiting the second fusion protein reactive enzyme thereby coupling the second fusion protein and the linker.

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