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公开(公告)号：US10351863B2
公开(公告)日：2019-07-16
申请号：US16018726
申请日：2018-06-26
Applicant: Novozymes A/S
Inventor： Carsten Lillelund Olsen
Abstract: The present invention relates to methods for constructing a recombinant fungal host cell comprising one or more copies of a polynucleotide construct integrated in its genome, said method comprising transforming a fungal host cell with an integrative polynucleotide construct comprising a first polynucleotide encoding a selectable marker, wherein the first polynucleotide, a 5′ untranslated region thereof and/or a riboswitch operably linked therewith comprises a spliceosomal intron which has 5 nucleotides or less between its branch site and its acceptor site; and a second polynucleotide encoding a polypeptide of interest; as well as suitable polynucleotide constructs, resulting fungal host cells and methods of manufacture.
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公开(公告)号：US20180298394A1
公开(公告)日：2018-10-18
申请号：US16018726
申请日：2018-06-26
Applicant: Novozymes A/S
Inventor： Carsten Lillelund Olsen
CPC classification number: C12N15/80 , C12N9/0044 , C12N9/20 , C12N9/88 , C12N15/67 , C12N15/902 , C12P21/00 , C12Y107/01003 , C12Y107/01004 , C12Y402/01011
Abstract: The present invention relates to methods for constructing a recombinant fungal host cell comprising one or more copies of a polynucleotide construct integrated in its genome, said method comprising transforming a fungal host cell with an integrative polynucleotide construct comprising a first polynucleotide encoding a selectable marker, wherein the first polynucleotide, a 5′ untranslated region thereof and/or a riboswitch operably linked therewith comprises a spliceosomal intron which has 5 nucleotides or less between its branch site and its acceptor site; and a second polynucleotide encoding a polypeptide of interest; as well as suitable polynucleotide constructs, resulting fungal host cells and methods of manufacture.
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公开(公告)号：US20150175981A1
公开(公告)日：2015-06-25
申请号：US14403355
申请日：2013-05-29
Applicant: Novozymes A/S
Inventor： Carsten Lillelund Olsen
CPC classification number: C12N15/80 , C12N9/0044 , C12N9/20 , C12N9/88 , C12N15/67 , C12N15/902 , C12P21/00 , C12Y107/01003 , C12Y107/01004 , C12Y402/01011
Abstract: The present invention relates to methods for constructing a recombinant fungal host cell comprising one or more copies of a polynucleotide construct integrated in its genome, said method comprising transforming a fungal host cell with an integrative polynucleotide construct comprising a first polynucleotide encoding a selectable marker, wherein the first polynucleotide, a 5′ untranslated region thereof and/or a riboswitch operably linked therewith comprises a spliceosomal intron which has 5 nucleotides or less between its branch site and its acceptor site; and a second polynucleotide encoding a polypeptide of interest; as well as suitable polynucleotide constructs, resulting fungal host cells and methods of manufacture.
Abstract translation: 本发明涉及用于构建重组真菌宿主细胞的方法，其包含整合在其基因组中的多核苷酸构建体的一个或多个拷贝，所述方法包括用包含编码可选择标记的第一多核苷酸的整合型多核苷酸构建体转化真菌宿主细胞，其中 第一个多核苷酸，其5'非翻译区和/或与其可操作地连接的核糖开关包含在其分支位点与其受体位点之间具有5个核苷酸或更少的剪接体内含子; 和编码目标多肽的第二个多核苷酸; 以及合适的多核苷酸构建体，得到的真菌宿主细胞和制造方法。
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Patent Agency Ranking
公开(公告)号：US20220073898A1
公开(公告)日：2022-03-10
申请号：US17416752
申请日：2019-12-17
Applicant: Novozymes A/S
Inventor： Richard Jan Steven Baerends , Carsten Lillelund Olsen , Josè Arnau
Abstract: The present invention provides means and methods for improving polypeptide expression yield by increasing the number of polypeptides that can be transported into the endoplasmic reticulum (ER) per translocation event.
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公开(公告)号：US10351861B2
公开(公告)日：2019-07-16
申请号：US15296433
申请日：2016-10-18
Applicant: Novozymes A/S
Inventor： Carsten Lillelund Olsen
Abstract: The present invention relates to methods for constructing a recombinant fungal host cell comprising one or more copies of a polynucleotide construct integrated in its genome, said method comprising transforming a fungal host cell with an integrative polynucleotide construct comprising a first polynucleotide encoding a selectable marker, wherein the first polynucleotide, a 5′ untranslated region thereof and/or a riboswitch operably linked therewith comprises a spliceosomal intron which has 5 nucleotides or less between its branch site and its acceptor site; and a second polynucleotide encoding a polypeptide of interest; as well as suitable polynucleotide constructs, resulting fungal host cells and methods of manufacture.
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公开(公告)号：US20170029830A1
公开(公告)日：2017-02-02
申请号：US15296433
申请日：2016-10-18
Applicant: Novozymes A/S
Inventor： Carsten Lillelund Olsen
CPC classification number: C12N15/80 , C12N9/0044 , C12N9/20 , C12N9/88 , C12N15/67 , C12N15/902 , C12P21/00 , C12Y107/01003 , C12Y107/01004 , C12Y402/01011
Abstract: The present invention relates to methods for constructing a recombinant fungal host cell comprising one or more copies of a polynucleotide construct integrated in its genome, said method comprising transforming a fungal host cell with an integrative polynucleotide construct comprising a first polynucleotide encoding a selectable marker, wherein the first polynucleotide, a 5′ untranslated region thereof and/or a riboswitch operably linked therewith comprises a spliceosomal intron which has 5 nucleotides or less between its branch site and its acceptor site; and a second polynucleotide encoding a polypeptide of interest; as well as suitable polynucleotide constructs, resulting fungal host cells and methods of manufacture.
Abstract translation: 本发明涉及用于构建重组真菌宿主细胞的方法，其包含整合在其基因组中的多核苷酸构建体的一个或多个拷贝，所述方法包括用包含编码可选择标记的第一多核苷酸的整合型多核苷酸构建体转化真菌宿主细胞，其中 第一个多核苷酸，其5'非翻译区和/或与其可操作地连接的核糖开关包含在其分支位点与其受体位点之间具有5个核苷酸或更少的剪接体内含子; 和编码目标多肽的第二个多核苷酸; 以及合适的多核苷酸构建体，得到的真菌宿主细胞和制造方法。
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公开(公告)号：US09487767B2
公开(公告)日：2016-11-08
申请号：US14403355
申请日：2013-05-29
Applicant: Novozymes A/S
Inventor： Carsten Lillelund Olsen
CPC classification number: C12N15/80 , C12N9/0044 , C12N9/20 , C12N9/88 , C12N15/67 , C12N15/902 , C12P21/00 , C12Y107/01003 , C12Y107/01004 , C12Y402/01011
Abstract: The present invention relates to methods for constructing a recombinant fungal host cell comprising one or more copies of a polynucleotide construct integrated in its genome, said method comprising transforming a fungal host cell with an integrative polynucleotide construct comprising a first polynucleotide encoding a selectable marker, wherein the first polynucleotide, a 5′ untranslated region thereof and/or a riboswitch operably linked therewith comprises a spliceosomal intron which has 5 nucleotides or less between its branch site and its acceptor site; and a second polynucleotide encoding a polypeptide of interest; as well as suitable polynucleotide constructs, resulting fungal host cells and methods of manufacture.
Abstract translation: 本发明涉及用于构建重组真菌宿主细胞的方法，其包含整合在其基因组中的多核苷酸构建体的一个或多个拷贝，所述方法包括用包含编码可选择标记的第一多核苷酸的整合型多核苷酸构建体转化真菌宿主细胞，其中 第一个多核苷酸，其5'非翻译区和/或与其可操作地连接的核糖开关包含在其分支位点与其受体位点之间具有5个核苷酸或更少的剪接体内含子; 和编码目标多肽的第二个多核苷酸; 以及合适的多核苷酸构建体，得到的真菌宿主细胞和制造方法。
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公开(公告)号：US20160304905A1
公开(公告)日：2016-10-20
申请号：US15101252
申请日：2014-12-03
Applicant: NOVOZYMES A/S
Inventor： Bjarne G. Hansen , Carsten Lillelund Olsen , Jesper Vind
CPC classification number: C12N15/902 , C12N15/80
Abstract: The present invention relates to a method for site-specific chromosomal integration of a gene library in a filamentous fungal host cell and a polynucleotide construct suitable for this purpose.
Abstract translation: 本发明涉及丝状真菌宿主细胞中基因文库的位点特异性染色体整合方法和适用于该目的的多核苷酸构建体。
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