公开(公告)号：US11203790B2

公开(公告)日：2021-12-21

申请号：US16656708

申请日：2019-10-18

Applicant: NOVOZYMES A/S

Inventor： Shikha Gupta ,  Allan Svendsen ,  Vivek Srivastava ,  Marco Malten ,  Thomas Agersten Poulsen ,  Preethi Ramaiya ,  Beth Nelson ,  Vasudeva Prahlada Rao ,  Padmavathi Balumuri

IPC: C12P19/02 ,  C12P19/14 ,  C12P19/20 ,  C12N9/34

Abstract: The present invention relates to glucoamylase variants, polynucleotides encoding the variants, methods of producing the variants, and methods of using the variants. Also described are the use of glucoamylases of the invention for starch conversion to produce fermentation products, such as ethanol, and syrups, such as glucose. The invention also relates to a composition comprising a glucoamylase variant of the invention.

公开(公告)号：US20190048413A1

公开(公告)日：2019-02-14

申请号：US16079141

申请日：2017-02-23

Applicant: NOVOZYMES A/S

Inventor： Marco Malten ,  Thomas Agersten Poulsen ,  Preethi Ramaiya ,  Michael Bargisen ,  Beth Nelson

IPC: C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6876

Abstract: The present invention enables the determination by NGS of the full nucleotide sequence of one or more polynucleotides of interest that are more than 800 bp in length after only one round of PCR amplification and subsequent fragmentation by adding at least one known oligonucleotide index to at least one end of each polynucleotide to produce elongated polynucleotides, wherein each known oligonucleotide index added to each polynucleotide is unique; fragmenting the elongated polynucleotides; sequencing the resulting fragments with paired forward and reverse reads using bridge amplification and next-generation sequencing; sorting the paired sequences based on the known oligonucleotide; and performing manual or in silico assembly of the sorted paired sequences, thereby providing the full coding sequence of each of the multitude of polynucleotides.

公开(公告)号：US20180298456A1

公开(公告)日：2018-10-18

申请号：US15767551

申请日：2016-10-12

Applicant: NOVOZYMES A/S

Inventor： Shikha Gupta ,  Allan Svendsen ,  Vivek Srivastava ,  Marco Malten ,  Thomas Agersten Poulsen ,  Preethi Ramaiya ,  Beth Nelson ,  Vasudeva P. Rao ,  Padmavathi Balumuri

IPC: C12P19/02 ,  C12P19/20 ,  C12N9/34 ,  C12P19/14

Abstract: The present invention relates to glucoamylase variants having an increase in specific activity and/or an increase in melting temperature measured by TSA of at least 0.5 degrees Celsius, and further wherein the variants have at least at least 85%, at least 90%, at least 95% identity, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US11046736B2

公开(公告)日：2021-06-29

申请号：US16319780

申请日：2017-07-19

Applicant: Novozymes A/S

Inventor： Hiroshi Teramoto ,  Kaihei Kojima ,  Preethi Ramaiya

IPC: C12N1/14 ,  C12N15/80 ,  C07K14/38 ,  C12P21/02

Abstract: The instant invention relates to filamentous fungal host cells producing and secreting a heterologous polypeptide of interest, said mutant host cell comprising and expressing a mutated ireA gene or a homologue thereof encoding a modified IreA polypeptide or a homologue thereof, said modified IreA polypeptide or homologue thereof comprising amino acid substitutions in 5 positions corresponding to positions 81 and 84 in the Aspergillus niger IreA amino acid sequence shown in SEQ ID NO:16, as well as methods of producing a polypeptide of interest in said cells and methods for constructing said cells.

公开(公告)号：US20190169238A1

公开(公告)日：2019-06-06

申请号：US16319780

申请日：2017-07-19

Applicant: Novozymes A/S

Inventor： Hiroshi Teramoto ,  Kaihei Kojima ,  Preethi Ramaiya

IPC: C07K14/38 ,  C12N15/80 ,  C12P21/02

Abstract: The instant invention relates to filamentous fungal host cells producing and secreting a heterologous polypeptide of interest, said mutant host cell comprising and expressing a mutated ireA gene or a homologue thereof encoding a modified IreA polypeptide or a homologue thereof, said modified IreA polypeptide or homologue thereof comprising amino acid substitutions in 5 positions corresponding to positions 81 and 84 in the Aspergillus niger IreA amino acid sequence shown in SEQ ID NO:16, as well as methods of producing a polypeptide of interest in said cells and methods for constructing said cells.