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    Intradermal delivery of active agents by needle-free injection and electroporation
    3.
    发明授权
    Intradermal delivery of active agents by needle-free injection and electroporation 有权
    通过无针注射和电穿孔活性剂的皮内递送

    公开(公告)号：US07171264B1

    公开(公告)日：2007-01-30

    申请号：US10339708

    申请日：2003-01-08

    申请人： Gunter A. Hofmann ,  Dietmar P. Rabussay ,  Lei Zhang

    发明人： Gunter A. Hofmann ,  Dietmar P. Rabussay ,  Lei Zhang

    IPC分类号： A61N1/30

    CPC分类号： A61N1/0424 ,  A61N1/0428 ,  A61N1/325

    摘要： Methods are proved for introducing a biologically active agent into cells of a subject by introducing the agent in a form suitable for electrotransport into a region of tissue of the subject using one or more needle-free injectors, and applying a pulsed electric field to the region of tissue, thereby causing electroporation of the region of tissue. The combination of needle-free injection and electroporation is sufficient to introduce the agent into cells in skin, muscle or mucosa. For example, the region of tissue can be contacted with two oppositely charged injectors, one acting as the donor electrode and one acting as the counter electrode, or a single injector and one or more electrodes can be used. In addition, needle-free injection may be used in combination with suitable non-invasive electrode configurations. The active agents delivered into cells using the invention method can be small molecules, polynucleotides, polypeptides, and the like.

    摘要翻译： 证明通过使用一种或多种无针注射器将适合于电转移到受试者组织区域的形式引入生物活性剂来引入生物活性剂的方法，以及向该区域施加脉冲电场 的组织，从而引起组织区域的电穿孔。 无针注射和电穿孔的组合足以将药剂引入皮肤，肌肉或粘膜中的细胞中。 例如，组织区域可以与两个相反电荷的注射器接触，一个充当施主电极，另一个充当对电极，或者可以使用单个注射器和一个或多个电极。 此外，无针注射可以与合适的非侵入性电极结构组合使用。 使用本发明方法递送到细胞中的活性剂可以是小分子，多核苷酸，多肽等。

    Method of electroporation-enhanced delivery of active agents
    4.
    发明授权
    Method of electroporation-enhanced delivery of active agents 有权
    电穿孔增强活性剂递送方法

    公开(公告)号：US06520950B1

    公开(公告)日：2003-02-18

    申请号：US09567404

    申请日：2000-05-08

    申请人： Gunter A. Hofmann ,  Dietmar P. Rabussay ,  Lei Zhang

    发明人： Gunter A. Hofmann ,  Dietmar P. Rabussay ,  Lei Zhang

    IPC分类号： A61M2500

    CPC分类号： A61N1/327 ,  A61N1/0412 ,  A61N1/0424 ,  A61N1/0428 ,  A61N1/0476 ,  A61N1/30 ,  A61N1/306 ,  A61N1/325 ,  Y10S977/906

    摘要： Methods are provided for introducing a biologically active agent into cells of a subject by injecting the agent with a needle-free injector and applying a pulsed electric field to the region of tissue to cause electrotransport of the agent into cells of the tissue. Preferably the agent is either ionized to some degree or contained in an ionized medium for electrotransport. The needle-free injector can serve as an electrode by which the pulsed electric field is applied to the region of tissue. The active agents delivered into cells by this method include small molecules, polynucleotides, polypeptides, and the like. Polynucleotides introduced into cells using this method can be used to accomplish gene therapy or to modulate expression of an endogenous gene.

    摘要翻译： 提供了通过用无针注射器注入药剂并将组织上的脉冲电场施加到组织区域以引起药物转移到组织细胞中而将生物活性剂引入受试者细胞的方法。 优选地，试剂在一定程度上被电离或包含在用于电转运的电离介质中。 无针注射器可用作电极，通过该电极将脉冲电场施加到组织区域。 通过该方法递送到细胞中的活性剂包括小分子，多核苷酸，多肽等。 使用该方法引入细胞的多核苷酸可用于完成基因治疗或调节内源基因的表达。

    Electrically induced vessel vasodilation
    6.
    发明授权
    Electrically induced vessel vasodilation 有权
    电导血管血管扩张

    公开(公告)号：US06865416B2

    公开(公告)日：2005-03-08

    申请号：US09969367

    申请日：2001-10-01

    申请人： Nagendu B. Dev ,  Sukhendu B. Dev ,  Gunter A. Hofmann

    发明人： Nagendu B. Dev ,  Sukhendu B. Dev ,  Gunter A. Hofmann

    IPC分类号： A61N1/30 ,  A61N1/32 ,  A61N1/36

    CPC分类号： A61N1/327 ,  A61N1/325 ,  Y10S977/883 ,  Y10S977/904 ,  Y10S977/905

    摘要： The invention provides methods for inducing or increasing the vasodilation of a vessel. The invention further provides methods for inducing or increasing the flow of fluid through a vessel. An electrical impulse is applied to the vessel in order to induce or increase vessel vasodilation or to induce or increase the flow of fluid through the vessel. In a particular embodiment, a novel double-balloon catheter system incorporating electroporation technology has been designed and is used to apply the electrical impulse endoluminally.

    摘要翻译： 本发明提供诱导或增加血管的血管舒张的方法。 本发明还提供了用于诱导或增加通过容器的流体流动的方法。 为了诱导或增加血管血管舒张或诱导或增加通过血管的流体流动，向血管施加电脉冲。 在一个具体的实施方案中，已经设计了一种包含电穿孔技术的新型双气囊导管系统，并且被用于内腔内施加电脉冲。

    Apparatus and method for the delivery of drugs and genes into tissue
    8.
    发明授权
    Apparatus and method for the delivery of drugs and genes into tissue 失效
    用于将药物和基因递送到组织中的装置和方法

    公开(公告)号：US06192270B1

    公开(公告)日：2001-02-20

    申请号：US09134245

    申请日：1998-08-14

    申请人： Gunter A. Hofmann ,  Dietmar Rabussay ,  Arnt Tonnessen

    发明人： Gunter A. Hofmann ,  Dietmar Rabussay ,  Arnt Tonnessen

    IPC分类号： A61N130

    CPC分类号： A61N1/0424 ,  A61N1/042 ,  A61N1/0436 ,  A61N1/044 ,  A61N1/30 ,  A61N1/325

    摘要： An electrode assembly for an apparatus for and a method of trans-surface molecular delivery, including a non-conductive carrier having a proximal surface, a distal surface, and a plurality of through holes from the proximal surface to the distal surface, a first electrode disposed on at least a portion of the distal surface and extending through at least a portion of the plurality of through holes and onto at least a portion of the proximal surface, a second electrode disposed on at least a portion of the proximal surface and in closely spaced relation with the first electrode for engaging a tissue surface through which to apply an electric field, and an insulating barrier that is disposed on at least a portion of the proximal surface of the carrier between the first electrode and the second electrode.

    摘要翻译： 一种用于反式表面分子传递的装置和方法的电极组件，包括具有近端表面，远端表面和从近端表面到远端表面的多个通孔的非导电载体，第一电极 设置在所述远端表面的至少一部分上并且延伸穿过所述多个通孔的至少一部分并且延伸到所述近端表面的至少一部分上，第二电极，设置在所述近端表面的至少一部分上并且紧密地 与用于接合施加电场的组织表面的第一电极间隔开的关系;以及设置在第一电极和第二电极之间的载体近端表面的至少一部分上的绝缘屏障。

    Method and apparatus for electrically assisted topical delivery of agents for cosmetic applications
    9.
    发明授权
    Method and apparatus for electrically assisted topical delivery of agents for cosmetic applications 失效
    用于化妆品应用的药剂的电辅助局部递送的方法和装置

    公开(公告)号：US06302874B1

    公开(公告)日：2001-10-16

    申请号：US09352818

    申请日：1999-07-13

    申请人： Lei Zhang ,  Gunter A. Hofmann

    发明人： Lei Zhang ,  Gunter A. Hofmann

    IPC分类号： A61M3100

    CPC分类号： A61N1/327 ,  A61N1/0424 ,  A61N1/0428 ,  A61N1/0436 ,  A61N1/0448 ,  A61N1/0468 ,  A61N1/325

    摘要： The present invention is based on the development of a method for cosmetic delivery of L-ascorbic acid-containing compositions to the layer of the skin wherein collagen formation takes place to enhance production of collagen and thereby combat some of the effects of aging and oxy-radical damage on skin. Sufficient electric pulses applied to a region of skin surface temporarily create new pathways through the lipid skin barrier, thereby delivering an L-ascorbic acid-containing composition through the lipid skin barrier and enhancing production of collagen in the region of the skin so treated. A handheld electrical pulser apparatus, optionally table-top adaptable, and methods of use for cosmetic treatment of degenerative skin conditions are provided by the invention.

    摘要翻译： 本发明基于开发用于将L-抗坏血酸的组合物美容递送到皮肤层的方法，其中发生胶原蛋白形成以增强胶原蛋白的产生，从而抵抗老化和氧化 - 对皮肤有根本的伤害。 施加到皮肤表面区域的足够的电脉冲暂时产生通过脂质皮肤屏障的新途径，从而通过脂质皮肤屏障输送含L-抗坏血酸的组合物，并增强如此治疗的皮肤区域中的胶原蛋白的产生。 本发明提供了一种手持式电脉冲发生器装置，任选桌面适应性，以及用于美容治疗退行性皮肤病症的方法。

    Method and apparatus for electrically assisted topical delivery of agents for cosmetic applications
    10.
    发明授权
    Method and apparatus for electrically assisted topical delivery of agents for cosmetic applications 失效
    用于化妆品应用的药剂的电辅助局部递送的方法和装置

    公开(公告)号：US06947791B2

    公开(公告)日：2005-09-20

    申请号：US09966390

    申请日：2001-09-27

    申请人： Lei Zhang ,  Gunter A. Hofmann

    发明人： Lei Zhang ,  Gunter A. Hofmann

    IPC分类号： A61N1/30 ,  A61N1/32

    CPC分类号： A61N1/327 ,  A61N1/0424 ,  A61N1/0428 ,  A61N1/0436 ,  A61N1/0448 ,  A61N1/0468 ,  A61N1/325

    摘要： The present invention is based on the development of a method for cosmetic delivery of L-ascorbic acid-containing compositions to the layer of the skin wherein collagen formation takes place to enhance production of collagen and thereby combat some of the effects of aging and oxy-radical damage on skin. Sufficient electric pulses applied to a region of skin surface temporarily create new pathways through the lipid skin barrier, thereby delivering an L-ascorbic acid-containing composition through the lipid skin barrier and enhancing production of collagen in the region of the skin so treated. A handheld electrical pulser apparatus, optionally table-top adaptable, and methods of use for cosmetic treatment of degenerative skin conditions are provided by the invention.

    摘要翻译： 本发明基于开发用于将L-抗坏血酸的组合物美容递送到皮肤层的方法，其中发生胶原蛋白形成以增强胶原蛋白的产生，从而抵抗老化和氧化 - 对皮肤有根本的伤害。 施加到皮肤表面区域的足够的电脉冲暂时产生通过脂质皮肤屏障的新途径，从而通过脂质皮肤屏障输送含L-抗坏血酸的组合物，并增强如此治疗的皮肤区域中的胶原蛋白的产生。 本发明提供了一种手持式电脉冲发生器装置，任选桌面适应性，以及用于美容治疗退行性皮肤病症的方法。