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    Vortex flow meter detector and vortex flow meter
    1.
    发明授权
    Vortex flow meter detector and vortex flow meter 失效
    涡街流量计检测器和涡街流量计

    公开(公告)号：US5736647A

    公开(公告)日：1998-04-07

    申请号：US690523

    申请日：1996-07-31

    申请人： Naoki Matsubara ,  Jun Tanimoto ,  Kenichi Takai ,  Shinsuke Imai

    发明人： Naoki Matsubara ,  Jun Tanimoto ,  Kenichi Takai ,  Shinsuke Imai

    IPC分类号： G01F1/32

    CPC分类号： G01F1/3263

    摘要： A vortex flowmeter detector is attached to or detachable from a vortex generation body for measurement of flow rate. The vortex flowmeter detector comprises a cylindrical oscillation tube having a bottom and a vortex detection part which is fixed by a fixing flange of the oscillation tube, wherein the oscillation tube is detachably inserted into a hole which is open to the vortex generation body at one end thereof, and the oscillation tube is fixed by a flange. An alternate pressure caused by a Karman vortex is introduced from both sides of the vortex generation body into the hole so as to oscillate the oscillation tube. This oscillation is received by a spring plate by way of an element cover, and it is transmitted to piezoelectric elements which are stuck to two chamfers of an elastic base material.

    摘要翻译： 涡流流量计检测器附接到涡流发生体或从涡流发生体上拆卸，用于测量流量。 涡街流量计检测器包括具有底部的圆柱形振荡管和由振荡管的固定凸缘固定的涡流检测部分，其中振荡管可拆卸地插入在一端向涡流发生体开放的孔中 并且振荡管由凸缘固定。 由卡门涡流引起的交替压力从涡流发生体的两侧引入到孔中以摆动振荡管。 该振动由弹簧板通过元件盖接收，并且传递到粘附到弹性基材的两个倒角的压电元件。

    Isozymes of lacrimator component synthase and gene encoding the same
    3.
    发明授权
    Isozymes of lacrimator component synthase and gene encoding the same 失效
    流感组分合成酶的同工酶和编码相同的基因

    公开(公告)号：US07371554B2

    公开(公告)日：2008-05-13

    申请号：US10344158

    申请日：2001-08-30

    申请人： Shinsuke Imai ,  Nobuaki Tsuge ,  Muneaki Tomotake

    发明人： Shinsuke Imai ,  Nobuaki Tsuge ,  Muneaki Tomotake

    IPC分类号： C12N9/00 ,  C12N9/88 ,  C07H21/04

    CPC分类号： C12N9/90 ,  C07K14/415 ,  C12N9/88

    摘要： It is an object of the present invention to provide isozymes of the lachrymatory factor producing enzyme, the amino acid sequences of these isozymes and a gene that codes for these amino acid sequences, and the present invention relates to three types of isozymes of the lachrymatory factor producing enzyme that contributes to the production of the lachrymatory factor that is present in onions and the like, amino acid sequences indicated by SEQ ID Nos. 1 to 3 which constitute the proteins or polypeptides of these isozymes, DNA indicated by SEQ ID Nos. 4 and 5 which contains base sequences that code for the abovementioned proteins or polypeptides, a method of producing the abovementioned isozymes, a recombinant vector which contains the abovementioned DNA, a transformant formed by transforming a host cell with the abovementioned recombinant vector, a method of producing proteins or polypeptides that have lachrymatory factor producing enzyme activity by culturing the abovementioned host cell, and anti-sense RNA which has a base sequence that is complementary to that of the mRNA corresponding to the abovementioned DNA.

    摘要翻译： 本发明的一个目的是提供产生泪液因子的酶的同工酶，这些同工酶的氨基酸序列和编码这些氨基酸序列的基因，本发明涉及三种类型的变应原因子的同功酶 产生有助于产生存在于洋葱等中的变应原因子的酶，由构成这些同功酶的蛋白质或多肽的SEQ ID NO.1至3表示的氨基酸序列，SEQ ID No.4所示的DNA 和5，其包含编码上述蛋白质或多肽的碱基序列，产生上述同功酶的方法，含有上述DNA的重组载体，通过用上述重组载体转化宿主细胞形成的转化体，制备 蛋白质或多肽，其具有通过培养上述宿主c产生酶活性的lachrymatory因子 和具有与对应于上述DNA的mRNA互补的碱基序列的反义RNA。

    Isozymes of lacrimator component synthase and gene encoding the same
    6.
    发明申请
    Isozymes of lacrimator component synthase and gene encoding the same 失效
    流感组分合成酶的同工酶和编码相同的基因

    公开(公告)号：US20050176126A1

    公开(公告)日：2005-08-11

    申请号：US10344158

    申请日：2001-08-30

    申请人： Shinsuke Imai ,  Nobuaki Tsuge ,  Muneaki Tomotake

    发明人： Shinsuke Imai ,  Nobuaki Tsuge ,  Muneaki Tomotake

    IPC分类号： C07K14/415 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N9/88 ,  C12N9/90 ,  C12N15/61 ,  C12N9/64 ,  C07H21/04

    CPC分类号： C12N9/90 ,  C07K14/415 ,  C12N9/88

    摘要： It is an object of the present invention to provide isozymes of the lachrymatory factor producing enzyme, the amino acid sequences of these isozymes and a gene that codes for these amino acid sequences, and the present invention relates to three types of isozymes of the lachrymatory factor producing enzyme that contributes to the production of the lachrymatory factor that is present in onions and the like, amino acid sequences indicated by SEQ ID Nos. 1 to 3 which constitute the proteins or polypeptides of these isozymes, DNA indicated by SEQ ID Nos. 4 and 5 which contains base sequences that code for the abovementioned proteins or polypeptides, a method of producing the abovementioned isozymes, a recombinant vector which contains the abovementioned DNA, a transformant formed by transforming a host cell with the abovementioned recombinant vector, a method of producing proteins or polypeptides that have lachrymatory factor producing enzyme activity by culturing the abovementioned host cell, and anti-sense RNA which has a base sequence that is complementary to that of the mRNA corresponding to the abovementioned DNA.

    摘要翻译： 本发明的一个目的是提供产生泪液因子的酶的同工酶，这些同工酶的氨基酸序列和编码这些氨基酸序列的基因，本发明涉及三种类型的变应原因子的同功酶 产生有助于产生存在于洋葱等中的变应原因子的酶，由构成这些同功酶的蛋白质或多肽的SEQ ID NO.1至3表示的氨基酸序列，SEQ ID No.4所示的DNA 和5，其包含编码上述蛋白质或多肽的碱基序列，产生上述同功酶的方法，含有上述DNA的重组载体，通过用上述重组载体转化宿主细胞形成的转化体，制备 蛋白质或多肽，其具有通过培养上述宿主c产生酶活性的lachrymatory因子 和具有与对应于上述DNA的mRNA互补的碱基序列的反义RNA。

    DNA and vector for repressing expression of gene of lachrymatory factor-producing enzyme, method for repressing expression of gene of lachrymatory factor-producing enzyme with them and vegetables having repressed expression of gene of lachrymatory factor-producing enzyme
    8.
    发明申请
    DNA and vector for repressing expression of gene of lachrymatory factor-producing enzyme, method for repressing expression of gene of lachrymatory factor-producing enzyme with them and vegetables having repressed expression of gene of lachrymatory factor-producing enzyme 失效
    用于抑制产生Lachrymatory因子的酶基因表达的DNA和载体，用它们抑制产生lachrymative因子的酶基因表达的方法和产生产生Lachrymatory因子的酶的基因的表达的蔬菜

    公开(公告)号：US20050022263A1

    公开(公告)日：2005-01-27

    申请号：US10932950

    申请日：2004-09-01

    申请人： Shinsuke Imai ,  Nobuaki Tsuge ,  Yasuhiro Kamata ,  Noriya Masamura ,  Jinji Shono ,  Kentaro Horie

    发明人： Shinsuke Imai ,  Nobuaki Tsuge ,  Yasuhiro Kamata ,  Noriya Masamura ,  Jinji Shono ,  Kentaro Horie

    IPC分类号： C12N9/90 ,  C12N15/29 ,  C12N15/82 ,  A01H1/00 ,  C07H21/04 ,  C12N5/04 ,  C12N9/02

    CPC分类号： C12N15/8243 ,  C12N9/90 ,  C12N15/8242

    摘要： The object of the present invention is to provide DNA and RNA designed on the basis of the sequence of an enzyme gene for repressing the expression of the enzyme gene generating a lachrymatory factor from a precursor of the lachrymatory factor, and also a vector required for introducing DNA for repressing the expression of the gene of the lachrymatory factor-producing enzyme into a vegetable. The present invention relates to DNA comprising at least one sequence selected from the following sequences and a regulatory sequence connected to said sequence so as to make the transcription possible: (a) a DNA sequence encoding a protein or a polypeptide of a lachrymatory factor-producing enzyme or a part of the DNA sequence in a sense orientation, an antisense orientation or both the orientations, wherein the protein or the polypeptide has an effect of converting 1-propenylsulfenic acid into the lachrymatory factor; (b) a regulatory sequence of a DNA in a vegetable genome determined on the basis of the DNA encoding the protein or the polypeptide of the lachrymatory factor-producing enzyme or a part of the regulatory sequence in a sense orientation, an antisense orientation or both the orientations; and (c) a DNA sequence located between the DNA encoding the protein or the polypeptide of the lachrymatory factor-producing enzyme and the regulatory sequence of the DNA in the vegetable genome DNA determined on the basis of the DNA encoding the protein or the polypeptide of the lachrymatory factor-producing enzyme or a part of the DNA sequence in a sense orientation, an antisense orientation or both the orientations.

    摘要翻译： 本发明的目的是提供基于酶基因序列设计的DNA和RNA，其用于抑制来自产生泪液因子的前体的产生泪液因子的酶基因的表达，以及引入所需的载体 用于将产生致命菌素的酶的基因的表达抑制成植物的DNA。 本发明涉及包含至少一个选自以下序列的序列的DNA，以及与所述序列连接的调控序列，以使转录成为可能：（a）编码产生产生泪液的因子的蛋白质或多肽的DNA序列 酶或一部分DNA序列在正义取向，反义方向或取向两者中，其中所述蛋白质或多肽具有将1-丙烯基亚磺酸转化为变应原因子的作用; （b）基于编码产生产生泪液因子的酶的蛋白质或多肽的DNA或有义方向，反义方向或两者的一部分调节序列确定的植物基因组DNA的调节序列 方向; 和（c）位于编码产生致命菌素的酶的蛋白质或多肽的DNA与植物基因组DNA中的DNA的调节序列之间的DNA序列，其基于编码所述蛋白质或多肽的DNA 衍生因子产生酶或部分DNA序列在正向，反义方向或取向上。

    Method for manufacturing blue pigment
    9.
    发明授权
    Method for manufacturing blue pigment 失效
    蓝色颜料的制造方法

    公开(公告)号：US5788758A

    公开(公告)日：1998-08-04

    申请号：US874409

    申请日：1997-06-13

    申请人： Hiroshi Sawada ,  Shinsuke Imai ,  Muneaki Tomotake ,  Kaori Akita

    发明人： Hiroshi Sawada ,  Shinsuke Imai ,  Muneaki Tomotake ,  Kaori Akita

    IPC分类号： A23L1/275 ,  C08K5/00

    CPC分类号： A23L5/48

    摘要： The present invention is intended to provide a method for manufacturing a natural blue pigment that can be used in food products and the like, and the present invention relates to a method for manufacturing a blue pigment, comprising the steps of combining and warming the reaction product of PeCSO and alliinase, an amino acid, and allicin in an aqueous solution. Other embodiments of the present invention include the aforementioned method for manufacturing a blue pigment, further comprising the steps of extracting the reaction product of PeCSO and alliinase with a water-insoluble organic solvent, and allowing the reaction product to further react with an amino acid in an aqueous solution with the resulting component, and subsequently adding allicin to the reaction mixture to develop blue color, as well as the aforementioned method for manufacturing a blue pigment, further comprising the steps of combining the reaction product of PeCSO and alliinase, an amino acid, and allicin in an aqueous solution, and subsequently heat treating these materials before they are warmed.

    摘要翻译： 本发明旨在提供可用于食品等的天然蓝色颜料的制造方法，本发明涉及一种蓝色颜料的制造方法，其特征在于，包括以下步骤：将反应产物 的PeCSO和蒜氨酸酶，氨基酸和大蒜素在水溶液中。 本发明的其它实施方案包括上述蓝色颜料的制造方法，其还包括以下步骤：用水不溶性有机溶剂萃取PeCSO和蒜氨酸酶的反应产物，并使反应产物进一步与 与所得组分的水溶液，然后将大蒜素加入到反应混合物中以形成蓝色，以及上述制备蓝色颜料的方法，还包括以下步骤：将PeCSO和蒜氨酸酶的反应产物，氨基酸 ，和大蒜素在水溶液中，然后在加热之前对这些材料进行热处理。