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    DNA and vector for repressing expression of gene of lachrymatory factor-producing enzyme, method for repressing expression of gene of lachrymatory factor-producing enzyme with them and vegetables having repressed expression of gene of lachrymatory factor-producing enzyme
    2.
    发明申请
    DNA and vector for repressing expression of gene of lachrymatory factor-producing enzyme, method for repressing expression of gene of lachrymatory factor-producing enzyme with them and vegetables having repressed expression of gene of lachrymatory factor-producing enzyme 失效
    用于抑制产生Lachrymatory因子的酶基因表达的DNA和载体，用它们抑制产生lachrymative因子的酶基因表达的方法和产生产生Lachrymatory因子的酶的基因的表达的蔬菜

    公开(公告)号：US20050022263A1

    公开(公告)日：2005-01-27

    申请号：US10932950

    申请日：2004-09-01

    申请人： Shinsuke Imai ,  Nobuaki Tsuge ,  Yasuhiro Kamata ,  Noriya Masamura ,  Jinji Shono ,  Kentaro Horie

    发明人： Shinsuke Imai ,  Nobuaki Tsuge ,  Yasuhiro Kamata ,  Noriya Masamura ,  Jinji Shono ,  Kentaro Horie

    IPC分类号： C12N9/90 ,  C12N15/29 ,  C12N15/82 ,  A01H1/00 ,  C07H21/04 ,  C12N5/04 ,  C12N9/02

    CPC分类号： C12N15/8243 ,  C12N9/90 ,  C12N15/8242

    摘要： The object of the present invention is to provide DNA and RNA designed on the basis of the sequence of an enzyme gene for repressing the expression of the enzyme gene generating a lachrymatory factor from a precursor of the lachrymatory factor, and also a vector required for introducing DNA for repressing the expression of the gene of the lachrymatory factor-producing enzyme into a vegetable. The present invention relates to DNA comprising at least one sequence selected from the following sequences and a regulatory sequence connected to said sequence so as to make the transcription possible: (a) a DNA sequence encoding a protein or a polypeptide of a lachrymatory factor-producing enzyme or a part of the DNA sequence in a sense orientation, an antisense orientation or both the orientations, wherein the protein or the polypeptide has an effect of converting 1-propenylsulfenic acid into the lachrymatory factor; (b) a regulatory sequence of a DNA in a vegetable genome determined on the basis of the DNA encoding the protein or the polypeptide of the lachrymatory factor-producing enzyme or a part of the regulatory sequence in a sense orientation, an antisense orientation or both the orientations; and (c) a DNA sequence located between the DNA encoding the protein or the polypeptide of the lachrymatory factor-producing enzyme and the regulatory sequence of the DNA in the vegetable genome DNA determined on the basis of the DNA encoding the protein or the polypeptide of the lachrymatory factor-producing enzyme or a part of the DNA sequence in a sense orientation, an antisense orientation or both the orientations.

    摘要翻译： 本发明的目的是提供基于酶基因序列设计的DNA和RNA，其用于抑制来自产生泪液因子的前体的产生泪液因子的酶基因的表达，以及引入所需的载体 用于将产生致命菌素的酶的基因的表达抑制成植物的DNA。 本发明涉及包含至少一个选自以下序列的序列的DNA，以及与所述序列连接的调控序列，以使转录成为可能：（a）编码产生产生泪液的因子的蛋白质或多肽的DNA序列 酶或一部分DNA序列在正义取向，反义方向或取向两者中，其中所述蛋白质或多肽具有将1-丙烯基亚磺酸转化为变应原因子的作用; （b）基于编码产生产生泪液因子的酶的蛋白质或多肽的DNA或有义方向，反义方向或两者的一部分调节序列确定的植物基因组DNA的调节序列 方向; 和（c）位于编码产生致命菌素的酶的蛋白质或多肽的DNA与植物基因组DNA中的DNA的调节序列之间的DNA序列，其基于编码所述蛋白质或多肽的DNA 衍生因子产生酶或部分DNA序列在正向，反义方向或取向上。

    FENUGREEK SEED HAVING REDUCED BITTER TASTE AND METHOD FOR PRODUCING THE SAME
    5.
    发明申请
    FENUGREEK SEED HAVING REDUCED BITTER TASTE AND METHOD FOR PRODUCING THE SAME 审中-公开
    具有减少叮咬味道的柚子种子及其生产方法

    公开(公告)号：US20100055241A1

    公开(公告)日：2010-03-04

    申请号：US12450906

    申请日：2008-04-18

    申请人： Yuki Nakano ,  Shohei Hoshino ,  Jinji Shono ,  Nobuaki Tsuge

    发明人： Yuki Nakano ,  Shohei Hoshino ,  Jinji Shono ,  Nobuaki Tsuge

    IPC分类号： A23L1/36 ,  A23L1/211

    CPC分类号： A23L25/20 ,  A23L11/33

    摘要： It is an object of the present invention to obtain fenugreek seeds having reduced bitter taste without causing significant changes in non-bitter components contained in fenugreek seeds.The present invention relates to a method for producing fenugreek seeds having reduced bitter taste, comprising allowing β-glucosidase to act on an eluate in which the components of fenugreek seeds have been eluted and then allowing the fenugreek seeds to absorb the eluate and β-glucosidase, and a food containing such fenugreek seeds.

    摘要翻译： 本发明的目的在于获得葫芦巴种子具有降低的苦味，而不会引起葫芦巴种子中所含的非苦味成分的显着变化。 本发明涉及一种生产具有降低苦味的葫芦巴种子的方法，其特征在于包括使葡萄糖苷酶作用于洗脱液中，其中胡芦巴种子的组分已被洗脱，然后使胡芦巴种子吸收洗脱液和 葡萄糖苷酶和含有这种葫芦巴种子的食物。

    Processed product of fenugreek seeds and method for producing the same
    7.
    发明授权
    Processed product of fenugreek seeds and method for producing the same 有权
    葫芦巴种子的加工产品及其生产方法

    公开(公告)号：US08287927B2

    公开(公告)日：2012-10-16

    申请号：US12596406

    申请日：2008-04-17

    申请人： Yuki Nakano ,  Shohei Hoshino ,  Jinji Shono ,  Nobuaki Tsuge

    发明人： Yuki Nakano ,  Shohei Hoshino ,  Jinji Shono ,  Nobuaki Tsuge

    IPC分类号： A61K36/00

    CPC分类号： C12P1/00 ,  A23L11/33 ,  A23L33/105 ,  A61K36/48

    摘要： It is an object of the present invention to enhance the α-amylase inhibitory activity of a fenugreek seed component. According to the present invention, the bitter taste of fenugreek seeds can be collaterally reduced.The present invention relates to a processed product of fenugreek seeds obtained by allowing β-glucosidase to act on a fenugreek seed-derived material and the like, and the method for producing the same.

    摘要翻译： 本发明的目的是提高葫芦巴种子成分的α-淀粉酶抑制活性。 根据本发明，葫芦巴种子的苦味可以相应减少。 本发明涉及通过使β-葡糖苷酶作用于葫芦巴种子衍生物质等而得到的葫芦巴种子的加工品及其制造方法。

    Isozymes of lacrimator component synthase and gene encoding the same
    9.
    发明申请
    Isozymes of lacrimator component synthase and gene encoding the same 失效
    流感组分合成酶的同工酶和编码相同的基因

    公开(公告)号：US20050176126A1

    公开(公告)日：2005-08-11

    申请号：US10344158

    申请日：2001-08-30

    申请人： Shinsuke Imai ,  Nobuaki Tsuge ,  Muneaki Tomotake

    发明人： Shinsuke Imai ,  Nobuaki Tsuge ,  Muneaki Tomotake

    IPC分类号： C07K14/415 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N9/88 ,  C12N9/90 ,  C12N15/61 ,  C12N9/64 ,  C07H21/04

    CPC分类号： C12N9/90 ,  C07K14/415 ,  C12N9/88

    摘要： It is an object of the present invention to provide isozymes of the lachrymatory factor producing enzyme, the amino acid sequences of these isozymes and a gene that codes for these amino acid sequences, and the present invention relates to three types of isozymes of the lachrymatory factor producing enzyme that contributes to the production of the lachrymatory factor that is present in onions and the like, amino acid sequences indicated by SEQ ID Nos. 1 to 3 which constitute the proteins or polypeptides of these isozymes, DNA indicated by SEQ ID Nos. 4 and 5 which contains base sequences that code for the abovementioned proteins or polypeptides, a method of producing the abovementioned isozymes, a recombinant vector which contains the abovementioned DNA, a transformant formed by transforming a host cell with the abovementioned recombinant vector, a method of producing proteins or polypeptides that have lachrymatory factor producing enzyme activity by culturing the abovementioned host cell, and anti-sense RNA which has a base sequence that is complementary to that of the mRNA corresponding to the abovementioned DNA.

    摘要翻译： 本发明的一个目的是提供产生泪液因子的酶的同工酶，这些同工酶的氨基酸序列和编码这些氨基酸序列的基因，本发明涉及三种类型的变应原因子的同功酶 产生有助于产生存在于洋葱等中的变应原因子的酶，由构成这些同功酶的蛋白质或多肽的SEQ ID NO.1至3表示的氨基酸序列，SEQ ID No.4所示的DNA 和5，其包含编码上述蛋白质或多肽的碱基序列，产生上述同功酶的方法，含有上述DNA的重组载体，通过用上述重组载体转化宿主细胞形成的转化体，制备 蛋白质或多肽，其具有通过培养上述宿主c产生酶活性的lachrymatory因子 和具有与对应于上述DNA的mRNA互补的碱基序列的反义RNA。

    GENE OF ENZYME HAVING ACTIVITY TO GENERATE LACHRYMATORY FACTOR
    10.
    发明申请
    GENE OF ENZYME HAVING ACTIVITY TO GENERATE LACHRYMATORY FACTOR 审中-公开
    具有活性的酵母基因生成LACHRYMATORY因子

    公开(公告)号：US20090081754A1

    公开(公告)日：2009-03-26

    申请号：US12051023

    申请日：2008-03-19

    申请人： Shinsuke IMAI ,  Nobuaki Tsuge ,  Muneaki Tomotake

    发明人： Shinsuke IMAI ,  Nobuaki Tsuge ,  Muneaki Tomotake

    IPC分类号： C12N9/00 ,  C12N15/52 ,  C12N15/74 ,  C07H21/02 ,  C12N1/00

    CPC分类号： C12N9/90 ,  C07K14/415 ,  C12N9/88

    摘要： It is an object of the present invention to provide isozymes of the lachrymatory factor producing enzyme, the amino acid sequences of these isozymes and a gene that codes for these amino acid sequences, and the present invention relates to three types of isozymes of the lachrymatory factor producing enzyme that contributes to the production of the lachrymatory factor that is present in onions and the like, amino acid sequences indicated by SEQ ID Nos. 1 to 3 which constitute the proteins or polypeptides of these isozymes, DNA indicated by SEQ ID Nos. 4 and 5 which contains base sequences that code for the abovementioned proteins or polypeptides, a method of producing the abovementioned isozymes, a recombinant vector which contains the abovementioned DNA, a transformant formed by transforming a host cell with the abovementioned recombinant vector, a method of producing proteins or polypeptides that have lachrymatory factor producing enzyme activity by culturing the abovementioned host cell, and anti-sense RNA which has a base sequence that is complementary to that of the mRNA corresponding to the abovementioned DNA.

    摘要翻译： 本发明的一个目的是提供产生泪液因子的酶的同工酶，这些同工酶的氨基酸序列和编码这些氨基酸序列的基因，本发明涉及三种类型的变应原因子的同功酶 产生有助于产生存在于洋葱等中的变应原因子的酶，由构成这些同功酶的蛋白质或多肽的SEQ ID NO.1至3表示的氨基酸序列，SEQ ID No.4所示的DNA 和5，其包含编码上述蛋白质或多肽的碱基序列，产生上述同功酶的方法，含有上述DNA的重组载体，通过用上述重组载体转化宿主细胞形成的转化体，制备 蛋白质或多肽，其具有通过培养上述宿主c产生酶活性的lachrymatory因子 和具有与对应于上述DNA的mRNA互补的碱基序列的反义RNA。