公开(公告)号：US20090062520A1

公开(公告)日：2009-03-05

申请号：US11896612

申请日：2007-09-04

申请人： Naoki Sugimoto ,  Daisuke Miyoshi ,  Mamiko Inoue

发明人： Naoki Sugimoto ,  Daisuke Miyoshi ,  Mamiko Inoue

IPC分类号： C07H21/04

CPC分类号： C12Q1/6811 ,  C12Q1/6813 ,  C12Q2525/301

摘要： The present invention provides a method of producing a DNA structure in which multiple quadruplex DNAs are linked, which includes (a) a step of mixing multiple DNA molecules having an antiparallel quadruplex structural part, and at least two single stranded sticky ends extended from the end of the quadruplex structural part, wherein the single stranded sticky end of the each DNA molecule has a base sequence that can form a duplex through interaction with the single stranded sticky end of other DNA molecule.

摘要翻译： 本发明提供了一种制备其中连接多个四链体DNA的DNA结构的方法，其包括（a）混合具有反平行四重结构部分的多个DNA分子的步骤和从末端延伸的至少两个单链粘性末端 的四重结构部分，其中每个DNA分子的单链粘性末端具有通过与其它DNA分子的单链粘性末端相互作用形成双链体的碱基序列。

公开(公告)号：US20070105139A1

公开(公告)日：2007-05-10

申请号：US11543037

申请日：2006-10-05

申请人： Junji Nishigaki ,  Hiroyuki Hirai ,  Naoki Sugimoto ,  Daisuke Miyoshi

发明人： Junji Nishigaki ,  Hiroyuki Hirai ,  Naoki Sugimoto ,  Daisuke Miyoshi

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6818 ,  C12Q2563/155 ,  C12Q2563/107

摘要： A fluorescent probe having a base sequence complementary to a specific sequence in a target nucleic acid, wherein the fluorescent probe has one end labeled with a nano particle fluorescent material, and the other end labeled with a fluorescent dye capable of fluorescence resonance energy transfer from the nano particle fluorescent material.

摘要翻译： 一种具有与目标核酸中的特定序列互补的碱基序列的荧光探针，其中所述荧光探针具有用纳米颗粒荧光材料标记的一端，另一端用能够从所述荧光共振能量转移的荧光染料标记 纳米颗粒荧光材料。

公开(公告)号：US08119347B2

公开(公告)日：2012-02-21

申请号：US12649948

申请日：2009-12-30

申请人： Hidenobu Yaku ,  Daisuke Miyoshi

发明人： Hidenobu Yaku ,  Daisuke Miyoshi

IPC分类号： C12Q1/68

CPC分类号： G01N33/5308 ,  C12Q1/48 ,  G01N33/583 ,  G01N2333/9128 ,  Y10T436/147777

摘要： A method is provided for specifically detecting a G-quadruplex, and the like. The method is characterized by including the steps of preparing a solution including an anionic planar phthalocyanine and mixing the solution with a sample solution to obtain a liquid mixture. The solution includes an anionic planar phthalocyanine. The method also includes a step of measuring the absorbance at 640 to 740 nm of the obtained liquid mixture.

摘要翻译： 提供了用于特异性检测G-quadruplex等的方法。 该方法的特征在于包括以下步骤：制备包含阴离子平面酞菁的溶液并将溶液与样品溶液混合以获得液体混合物。 该溶液包括阴离子平面酞菁。 该方法还包括测量所得液体混合物在640至740nm处的吸光度的步骤。

公开(公告)号：US20100261246A1

公开(公告)日：2010-10-14

申请号：US12710837

申请日：2010-02-23

申请人： Hidenobu YAKU ,  Daisuke Miyoshi

发明人： Hidenobu YAKU ,  Daisuke Miyoshi

IPC分类号： C12N9/99

CPC分类号： C12N9/99 ,  C12Q1/6844 ,  C12Q2521/113 ,  C12Q2523/10

摘要： An object of the present invention is to provide a method for inhibiting a DNA extension reaction by telomerase.More specifically, the present invention relates to a method for inhibiting a DNA extension reaction by telomerase, the method being characterized by including the step of adding an anionic phthalocyanine to a solution containing telomerase, a DNA to be a substrate of a telomerase reaction, and dNTPs.

摘要翻译： 本发明的目的是提供一种通过端粒酶抑制DNA延伸反应的方法。 更具体地，本发明涉及一种通过端粒酶抑制DNA延伸反应的方法，该方法的特征在于包括将阴离子酞菁加入到含有端粒酶的溶液，作为端粒酶反应的底物的DNA和 dNTPs。

公开(公告)号：US08101357B2

公开(公告)日：2012-01-24

申请号：US12710837

申请日：2010-02-23

申请人： Hidenobu Yaku ,  Daisuke Miyoshi

发明人： Hidenobu Yaku ,  Daisuke Miyoshi

IPC分类号： C12Q1/68

CPC分类号： C12N9/99 ,  C12Q1/6844 ,  C12Q2521/113 ,  C12Q2523/10

摘要： An object of the present invention is to provide a method for inhibiting a DNA extension reaction by telomerase.More specifically, the present invention relates to a method for inhibiting a DNA extension reaction by telomerase, the method being characterized by including the step of adding an anionic phthalocyanine to a solution containing telomerase, a DNA to be a substrate of a telomerase reaction, and dNTPs.

摘要翻译： 本发明的目的是提供一种通过端粒酶抑制DNA延伸反应的方法。 更具体地，本发明涉及一种通过端粒酶抑制DNA延伸反应的方法，该方法的特征在于包括将阴离子酞菁加入到含有端粒酶的溶液，作为端粒酶反应的底物的DNA和 dNTPs。

公开(公告)号：US08232054B2

公开(公告)日：2012-07-31

申请号：US13335498

申请日：2011-12-22

申请人： Hidenobu Yaku ,  Daisuke Miyoshi

发明人： Hidenobu Yaku ,  Daisuke Miyoshi

IPC分类号： C12Q1/68

CPC分类号： G01N33/5308 ,  C12Q1/48 ,  G01N33/583 ,  G01N2333/9128 ,  Y10T436/147777

摘要： A method is provided for specifically detecting a G-quadruplex, and the like. The method is characterized by including the steps of preparing a solution including an anionic planar phthalocyanine and mixing the solution with a sample solution to obtain a liquid mixture. The solution includes an anionic planar phthalocyanine. The method also includes a step of measuring the absorbance at 640 to 740 nm of the obtained liquid mixture.

公开(公告)号：US20100173306A1

公开(公告)日：2010-07-08

申请号：US12649948

申请日：2009-12-30

申请人： Hidenobu YAKU ,  Daisuke Miyoshi

发明人： Hidenobu YAKU ,  Daisuke Miyoshi

IPC分类号： C12Q1/68 ,  G01N33/48

CPC分类号： G01N33/5308 ,  C12Q1/48 ,  G01N33/583 ,  G01N2333/9128 ,  Y10T436/147777

摘要： The present invention provides a method for specifically detecting a G-quadruplex, and the like.The method for detecting a G-quadruplex of the present invention is characterized by including the steps of: (a) preparing a solution including an anionic planar phthalocyanine; (b) mixing the solution including an anionic planar phthalocyanine with the sample solution; and (c) measuring the absorbance at 640 to 740 nm of a liquid mixture obtained following the step (b).

摘要翻译： 本发明提供了特异性检测G-四链体等的方法。 检测本发明的G-四链体的方法的特征在于包括以下步骤：（a）制备包括阴离子平面酞菁的溶液; （b）将包含阴离子平面酞菁的溶液与样品溶液混合; 和（c）测量在步骤（b）之后获得的液体混合物在640至740nm处的吸光度。