公开(公告)号：US20160320305A1

公开(公告)日：2016-11-03

申请号：US14741629

申请日：2015-06-17

Applicant: National Central University

Inventor： Szu-Yu CHEN ,  Poyu SU ,  Chiao-Sheng LU ,  Yu John HSU

IPC: G01N21/64 ,  G01J3/28 ,  G01J3/12 ,  G01J3/02 ,  G01J3/10

CPC classification number: G01N21/6458 ,  G01J3/0208 ,  G01J3/021 ,  G01J3/0229 ,  G01J3/0267 ,  G01J3/04 ,  G01J3/06 ,  G01J3/10 ,  G01J3/12 ,  G01J3/2803 ,  G01J3/2823 ,  G01J3/4406 ,  G01J2003/2813 ,  G01J2003/282 ,  G01N2021/6421 ,  G01N2021/6423 ,  G01N2021/6476 ,  G01N2021/6478

Abstract: A fluorescence hyperspectral microscopy system featuring structured illumination and parallel recording includes a light projection sub-system, a detection sub-system, and an electrical controller. The light projection sub-system includes a digital light processing (DLP) module for generating linear excitation light, a first lens set, an optical path allocation element, and an objective lens. The detection sub-system includes a second lens set, a frequency-dividing reflection element, a two-dimensional light detector, and a light collection element. With the detection sub-system performing detection in conjunction with the light projection sub-system, and the electrical controller controlling the DLP module, a two-dimensional moving platform, and the two-dimensional light detector, the fluorescence hyperspectral microscopy system provides increased resolution and can obtain accurate information in spatial and spectral dimensions and hence a four-dimensional hyperspectral image of the object under detection.

Abstract translation: 具有结构照明和并行记录的荧光高光谱显微系统包括光投影子系统，检测子系统和电控制器。 光投影子系统包括用于产生线性激发光的数字光处理（DLP）模块，第一透镜组，光路分配元件和物镜。 检测子系统包括第二透镜组，分频反射元件，二维光检测器和光收集元件。 通过检测子系统与光投影子系统进行检测，控制DLP模块的电气控制器，二维移动平台和二维光检测器，荧光高光谱显微系统提供更高的分辨率 并且可以在空间和光谱维度上获得准确的信息，并因此获得被检测物体的四维高光谱图像。