公开(公告)号：US09618452B2

公开(公告)日：2017-04-11

申请号：US14741629

申请日：2015-06-17

Applicant: National Central University

Inventor： Szu-Yu Chen ,  Poyu Su ,  Chiao-Sheng Lu ,  Yu John Hsu

IPC: G01N21/64 ,  G01J3/02 ,  G01J3/10 ,  G01J3/12 ,  G01J3/28

CPC classification number: G01N21/6458 ,  G01J3/0208 ,  G01J3/021 ,  G01J3/0229 ,  G01J3/0267 ,  G01J3/04 ,  G01J3/06 ,  G01J3/10 ,  G01J3/12 ,  G01J3/2803 ,  G01J3/2823 ,  G01J3/4406 ,  G01J2003/2813 ,  G01J2003/282 ,  G01N2021/6421 ,  G01N2021/6423 ,  G01N2021/6476 ,  G01N2021/6478

Abstract: A fluorescence hyperspectral microscopy system featuring structured illumination and parallel recording includes a light projection sub-system, a detection sub-system, and an electrical controller. The light projection sub-system includes a digital light processing (DLP) module for generating linear excitation light, a first lens set, an optical path allocation element, and an objective lens. The detection sub-system includes a second lens set, a frequency-dividing reflection element, a two-dimensional light detector, and a light collection element. With the detection sub-system performing detection in conjunction with the light projection sub-system, and the electrical controller controlling the DLP module, a two-dimensional moving platform, and the two-dimensional light detector, the fluorescence hyperspectral microscopy system provides increased resolution and can obtain accurate information in spatial and spectral dimensions and hence a four-dimensional hyperspectral image of the object under detection.