公开(公告)号：US11359178B2

公开(公告)日：2022-06-14

申请号：US17268234

申请日：2019-08-23

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Yuzo Takayama ,  Yasuyuki Kida

IPC: C12N5/0793

Abstract: The present invention provides a method for producing parasympathetic neurons from neural crest cells or autonomic neural progenitor cells derived therefrom, comprising a step of culturing the neural crest cells or autonomic neural progenitor cells derived therefrom in the presence of a cAMP production promoter, a BDNF signaling pathway activator, a GDNF signaling pathway activator, an NGF signaling pathway activator, an NT-3 signaling pathway activator, vitamin C, a protein kinase C activator, and a retinoic acid receptor agonist.

公开(公告)号：US20210171905A1

公开(公告)日：2021-06-10

申请号：US17268234

申请日：2019-08-23

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Yuzo Takayama ,  Yasuyuki Kida

IPC: C12N5/0793

Abstract: The present invention provides a method for producing parasympathetic neurons from neural crest cells or autonomic neural progenitor cells derived therefrom, comprising a step of culturing the neural crest cells or autonomic neural progenitor cells derived therefrom in the presence of a cAMP production promoter, a BDNF signaling pathway activator, a GDNF signaling pathway activator, an NGF signaling pathway activator, an NT-3 signaling pathway activator, vitamin C, a protein kinase C activator, and a retinoic acid receptor agonist.

公开(公告)号：US20180155682A1

公开(公告)日：2018-06-07

申请号：US15578454

申请日：2016-04-26

Applicant: National Institute of Advanced Industrial Science and Technology

Inventor： Yasuyuki Kida ,  Yuzo Takayama

IPC: C12N5/0793

CPC classification number: C12N5/0619 ,  C12N1/00 ,  C12N5/00 ,  C12N2501/155 ,  C12N2501/30 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2501/48 ,  C12N2506/03

Abstract: Provided is a method for inducing differentiation of neural crest cells into neurons of the autonomic nervous system, the method including the step of culturing neural crest cells in the presence of at least one of a BMP signaling pathway activator, an SHH signaling pathway inhibitor, and a Wnt signaling pathway inhibitor.

公开(公告)号：US11186821B2

公开(公告)日：2021-11-30

申请号：US15578454

申请日：2016-04-26

Applicant: National Institute of Advanced Industrial Science and Technology

Inventor： Yasuyuki Kida ,  Yuzo Takayama

IPC: C12N5/00 ,  C12N5/0793 ,  C12N1/00

Abstract: Provided is a method for inducing differentiation of neural crest cells into neurons of the autonomic nervous system, the method including the step of culturing neural crest cells in the presence of at least one of a BMP signaling pathway activator, an SHH signaling pathway inhibitor, and a Wnt signaling pathway inhibitor.

公开(公告)号：US20240201067A1

公开(公告)日：2024-06-20

申请号：US18555186

申请日：2022-03-22

Applicant: National Institute of Advanced Industrial Science and Technology

Inventor： Yuka Akagi ,  Yasuyuki Kida ,  Nobuhito Mori ,  Yuzo Takayama

IPC: G01N15/1434 ,  G01N15/01

CPC classification number: G01N15/1434 ,  G01N15/01

Abstract: Provided is a spectral data acquisition method which can perform laser irradiation more uniformly while further reducing cell damage. The spectral data acquisition method involves receiving, by means of a spectroscope, Raman scattered light output from a cell by spirally irradiating an area in which the cell is present with laser light from the laser light source, and detecting a Raman spectrum in response to the received Raman scattered light by means of a charge-coupled device (CCD) detector.