公开(公告)号：US20240344959A1

公开(公告)日：2024-10-17

申请号：US18291952

申请日：2022-07-29

Applicant: TECHNISCHE UNIVERSITÄT MÜNCHEN

Inventor： Oliver HAYDEN ,  Johanna ERBER ,  Sebastian RASCH ,  Tobias LAHMER ,  Stefan RÖHRL ,  Christian KLENK

IPC: G01N15/1404 ,  G01N15/01 ,  G01N15/1433

CPC classification number: G01N15/1404 ,  G01N15/01 ,  G01N15/1433 ,  G01N2015/016 ,  G01N2015/018 ,  G01N2015/1413

Abstract: Disclosed herein is a method for detecting cell aggregates of biological cells using a quantitative phase-contrast microscope and a device for detecting cell aggregates of biological cells using said method. The method comprises preparing a suspension comprising biological cells from a sample. A flow of the suspension is generated along a microfluidic channel to viscoelastically and/or hydrodynamically focus cell aggregates in the suspension in a focal plane of the quantitative phase-contrast microscope. One or more phase shift images of the biological cells in the suspension are taken using the quantitative phase-contrast microscope. Cell aggregates in the one or more phase shift images are identified. The sample is a whole blood sample or a blood fraction sample and identifying cell aggregates in the one or more phase shift images comprises identifying platelet aggregates in the one or more phase shift images.

公开(公告)号：US12066369B2

公开(公告)日：2024-08-20

申请号：US18417986

申请日：2024-01-19

Applicant: Slingshot Biosciences, Inc.

Inventor： Jeffrey Kim ,  Oliver Liu ,  Jeremy Agresti ,  Anh Tuan Nguyen

IPC: G01N15/10 ,  C12Q1/04 ,  G01N1/28 ,  G01N15/14 ,  G01N15/1434 ,  G01N15/01 ,  G01N15/149

CPC classification number: G01N15/1012 ,  C12Q1/04 ,  G01N1/28 ,  G01N15/1434 ,  G01N15/1459 ,  G01N2001/2893 ,  G01N15/01 ,  G01N2015/1006 ,  G01N2015/1014 ,  G01N15/149

Abstract: Synthetic human cell mimic hydrogel particles and their use in cytometric or coulter device applications are described. The synthetic human cell mimic hydrogel particles described herein are selectively tunable to have at least one optical, volumetric, or capacitance property that is substantially similar to a corresponding optical, volumetric, or capacitance property of the human cell mimic hydrogel particle's natural biological cell counterpart.

公开(公告)号：US12031896B2

公开(公告)日：2024-07-09

申请号：US17425414

申请日：2020-01-24

Applicant: UNIVERSITY OF VIRGINIA PATENT FOUNDATION

Inventor： Nathan Swami ,  Walter Varhue ,  Vahid Farmehini

IPC: B01L3/00 ,  G01N15/01 ,  G01N15/1031 ,  G01N33/483 ,  G01R27/16

CPC classification number: G01N15/1031 ,  B01L3/502715 ,  B01L3/502761 ,  G01N33/4833 ,  G01R27/16 ,  B01L2200/0647 ,  B01L2300/0627 ,  G01N15/01

Abstract: Disclosed herein are methods and systems that facilitate the integration of on-chip impedance sensors and measurement circuitries for quantifying the impedance/frequency response of microfluidic device under the same, or similar, conditions used for particle manipulation. The methods and systems can use a microfluidic chip comprising a microfluidic channel with one or more electric-field-generating structures located therein, including a first electric-field-generating structure, wherein the one or more electric-field-generating structures is configured to selectively polarize or manipulate biologic or particle components flowing within the microfluidic channel. The method and system can also employ a circuit configured for automated determination and quantification of parasitic voltage drops during AC electrokinetic particle manipulation, without the need to use valuable biological samples or model particles. The determined impedance response can be used to assess efficacy of the microfluidic device geometry as well as to provide control signals to inform downstream cell separation decisions.

公开(公告)号：US20240198339A1

公开(公告)日：2024-06-20

申请号：US18595927

申请日：2024-03-05

Applicant: The General Hospital Corporation

Inventor： Ravi Kapur ,  Kyle C. Smith ,  Mehmet Toner

IPC: B01L3/00 ,  A61K35/28 ,  G01N1/40 ,  G01N15/00 ,  G01N15/01 ,  G01N15/02 ,  G01N15/06 ,  G01N15/14 ,  G01N15/149

CPC classification number: B01L3/502761 ,  A61K35/28 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  G01N1/4077 ,  G01N15/0255 ,  G01N15/0618 ,  G01N15/1484 ,  B01L3/502776 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0877 ,  B01L2300/185 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N2001/4088 ,  G01N2015/0053 ,  G01N15/01 ,  G01N2015/0288 ,  G01N2015/1486 ,  G01N15/149 ,  G01N2015/1493

Abstract: A microfluidic device includes: a first microfluidic channel; a second microfluidic channel extending along the first microfluidic channel; and a first array of islands separating the first microfluidic channel from the second microfluidic channel, in which each island is separated from an adjacent island in the array by an opening that fluidly couples the first microfluidic channel to the second microfluidic channel, in which the first microfluidic channel, the second microfluidic channel, and the islands are arranged so that a fluidic resistance of the first microfluidic channel increases relative to a fluidic resistance of the second microfluidic channel along a longitudinal direction of the first microfluidic channel such that, during use of the microfluidic device, a portion of a fluid sample flowing through the first microfluidic channel passes through one or more of the openings between adjacent islands into the second microfluidic channel.

公开(公告)号：US12007321B2

公开(公告)日：2024-06-11

申请号：US17200728

申请日：2021-03-12

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Dino Di Carlo ,  Daniel R. Gossett ,  Henry T. K. Tse ,  Aram Chung

IPC: G01N15/14 ,  G01N15/1404 ,  G01N15/1434 ,  G01N21/64 ,  G01N33/50 ,  G06T7/00 ,  G01N15/01 ,  G01N15/10

CPC classification number: G01N15/147 ,  G01N15/1404 ,  G01N15/1434 ,  G01N15/1436 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/6428 ,  G01N21/645 ,  G01N33/50 ,  G01N33/5091 ,  G06T7/0004 ,  G01N15/01 ,  G01N2015/1006 ,  G01N2015/1415 ,  G01N2015/1495 ,  G01N21/64 ,  G01N2021/6439 ,  G06T7/0016

Abstract: A system for deforming and analyzing a plurality of particles carried in a sample volume includes a substrate defining an inlet, configured to receive the sample volume, and an outlet; and a fluidic pathway fluidly coupled to the inlet and the outlet. The fluidic pathway includes a delivery region configured to receive the plurality of particles from the inlet and focus the plurality of particles from a random distribution to a focused state, a deformation region defining an intersection located downstream of the delivery region and coupled to the outlet, and wherein the deformation region is configured to receive the plurality of particles from the delivery region and to transmit each particle in the plurality of particles into the intersection from a single direction, a first branch fluidly coupled to the deformation region and configured to transmit a first flow into the intersection, and a second branch fluidly coupled to the deformation region and configured to transmit a second flow, substantially opposing the first flow, into the intersection, wherein the first flow and the second flow are configured to induce extension of one or more particles in the plurality of particles.

公开(公告)号：US20240117417A1

公开(公告)日：2024-04-11

申请号：US18261606

申请日：2022-01-14

Applicant: Secondcell Bio, LLC ,  Chromocell Corporation

Inventor： Dennis James SAWCHUK ,  Olga Anatoliyevna DEDOVA ,  Kambiz SHEKDAR

IPC: C12Q1/6841 ,  C12N15/85 ,  G01N15/01 ,  G01N15/1434

CPC classification number: C12Q1/6841 ,  C12N15/85 ,  G01N15/01 ,  G01N15/1434 ,  G01N2015/1006

Abstract: The present disclosure relates to novel expression vectors, host cells and methods useful to produce cells comprising one or more sequences of interest.

公开(公告)号：US11925934B2

公开(公告)日：2024-03-12

申请号：US16918349

申请日：2020-07-01

Applicant: GEORGIA TECH RESEARCH CORPORATION

Inventor： Todd Sulchek ,  Alexander Alexeev ,  Gonghao Wang

IPC: B01L3/00 ,  G01N15/02 ,  G01N33/48 ,  G01N33/49 ,  G01N33/50

CPC classification number: B01L3/502761 ,  G01N15/0272 ,  G01N33/48 ,  G01N33/5005 ,  B01L3/502776 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/086 ,  G01N15/01 ,  G01N2015/0288 ,  G01N33/491

Abstract: Microfluidic devices for cell sorting or cell fractionation are disclosed. A microfluidic device can comprise one or more inlets, a first wall and a second wall, and two or more outlets. The first and second walls can be substantially planar to each other and the first wall having can have a plurality of ridges protruding from the first wall and defining a compression gap between the ridge and a surface of the second wall. The microfluidic device can also be a cell sorting device for sorting a plurality of cells based on one or more biophysical cellular properties including size, elasticity, viscosity, and/or viscoelasticity wherein the cells are subjected to one or more compressions due to the compression gap. Also disclosed are methods for cell sorting based on a variety of biophysical cellular properties.

公开(公告)号：US20240357232A1

公开(公告)日：2024-10-24

申请号：US18740301

申请日：2024-06-11

Applicant: Beckman Coulter, Inc.

Inventor： Jiuliu LU ,  Bian QIAN ,  John S. RILEY ,  Bart WANDERS ,  Ken GOOD

IPC: H04N23/67 ,  G01N15/01 ,  G01N15/1434 ,  G06T3/40 ,  G06T7/00

CPC classification number: H04N23/67 ,  G01N15/01 ,  G01N15/1436 ,  G06T3/40 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/10148 ,  G06T2207/20081 ,  G06T2207/20084 ,  G06T2207/30024

Abstract: A focusing distance (e.g., a distance between a focal plane of a camera used when n image was captured and the actual focal plane for an in-focus image) in a visual analysis system may be determined by subjecting one or more images captured by such a system to a multi-layer analysis. In such an analysis, an input may be subjected to one or more convolution filters, and the ultimate result of such convolution may be provided to a dense layer which can provide the focusing distance. This focusing distance may then be used to (re)focus a camera or for other purposes (e.g., generating an alert).

公开(公告)号：US12123822B2

公开(公告)日：2024-10-22

申请号：US18013965

申请日：2021-07-05

Applicant: DAEGU GYEONGBUK INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Jae Youn Hwang ,  Sang Yeon Youn

IPC: G01N15/14 ,  G01B11/06 ,  G01N15/1434 ,  G06T7/60 ,  H04N23/56 ,  G01N15/01 ,  G01N15/10

CPC classification number: G01N15/1434 ,  G01B11/06 ,  G06T7/60 ,  H04N23/56 ,  G01N15/01 ,  G01N2015/1006 ,  G01N2015/144 ,  G01N2015/1495 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/30096

Abstract: An imaging system according to an embodiment may include an ultrasonic oscillation part configured to apply ultrasonic waves to a sample, an image acquisition part configured to acquire a plurality of images of the sample deformed by the ultrasonic waves, and a computation part configured to compute a deformation rate on the basis of a change in thickness of the sample from the plurality of images, in which the computation part computes an elastic modulus of the sample on the basis of intensity of the ultrasonic waves and the deformation rate of the sample.

公开(公告)号：US12111317B2

公开(公告)日：2024-10-08

申请号：US16620580

申请日：2018-06-08

Applicant: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA

Inventor： Lewis Chodosh ,  Elizabeth Chislock

IPC: C07K16/32 ,  A61K49/00 ,  G01N15/10 ,  G01N15/14 ,  G01N33/574 ,  G01N15/01

CPC classification number: G01N33/57415 ,  A61K49/0058 ,  C07K16/32 ,  G01N15/10 ,  G01N15/1459 ,  G01N15/01 ,  G01N2015/1006 ,  G01N2800/50

Abstract: The present invention provides compositions and methods for detecting and isolating disseminated tumor cells (DTCs) from breast cancer in a bone marrow aspirate of a subject. The invention further provides methods of treating breast cancer in the subject.