公开(公告)号：US20150232502A1

公开(公告)日：2015-08-20

申请号：US14627396

申请日：2015-02-20

申请人： National Research Council of Canada ,  University of Massachusetts Medical School

发明人： Ian Schoenhofen ,  Dennis M. Whitfield ,  Sanjay RAM

IPC分类号： C07H19/10 ,  G01N33/569 ,  C12Q1/18 ,  C12P19/30 ,  A61K31/7068

CPC分类号： C07H19/10 ,  A61K31/7068 ,  C12Q1/18

摘要： N. gonorrhoeae has become resistant to almost every conventional antibiotic. Described herein is the use of CMP-nonulosonate analogues to counter gonococcal complement evasion. The nonulosonate sugar is incorporated into the lipooligosaccharide of the N. gonorrhoeae which in turn reduces the serum resistance of the bacteria. This provides a novel therapeutic strategy against the global threat of multi-drug resistant gonorrhea.

摘要翻译： 淋病奈瑟氏球菌对几乎每一种常规的抗生素都具有抗性。 本文描述的是使用CMP-非罗洛糖醇类似物来对抗淋菌性补体逃避。 非罗洛克糖被并入淋病奈瑟氏球菌的低温寡糖中，这又降低了细菌的血清耐药性。 这为抗多药耐药性淋病的全球威胁提供了一种新颖的治疗策略。

公开(公告)号：US09765106B2

公开(公告)日：2017-09-19

申请号：US14627396

申请日：2015-02-20

申请人： National Research Council of Canada ,  University of Massachusetts Medical School

发明人： Ian Schoenhofen ,  Dennis M. Whitfield ,  Sanjay Ram

IPC分类号： C07H19/10 ,  A61K31/7068 ,  C12Q1/18

CPC分类号： C07H19/10 ,  A61K31/7068 ,  C12Q1/18

摘要： N. gonorrhoeae has become resistant to almost every conventional antibiotic. Described herein is the use of CMP-nonulosonate analogs to counter gonococcal complement evasion. The nonulosonate sugar is incorporated into the lipooligosaccharide of the N. gonorrhoeae which in turn reduces the serum resistance of the bacteria. This provides a novel therapeutic strategy against the global threat of multi-drug resistant gonorrhea.

公开(公告)号：US20170158728A1

公开(公告)日：2017-06-08

申请号：US15325640

申请日：2015-07-10

申请人： National Research Council of Canada

发明人： Dennis M. Whitfield ,  G. Dennis Sprott ,  Lakshmi Krishnan

IPC分类号： C07H15/04 ,  A61K39/00 ,  A61K39/39

CPC分类号： C07H15/04 ,  A61K39/0011 ,  A61K39/39 ,  A61K2039/54 ,  A61K2039/55555 ,  A61K2039/55572 ,  A61K2039/57

摘要： A synthetic charged glycolipid is described comprising a sulfated saccharide group covalently linked to the tree sn-1 hydroxyl group of the glycerol backbone of an archaeal core lipid via a beta linkage. The synthetic charged glycolipids include compounds of formula I wherein n is 0 or 1; R is hydrogen or hydroxyl; and Y is hydrogen or a sulfate group, at least one Y being a sulfate group; and including pharmaceutically acceptable salts thereof. The sulfated glycolipid produces stable archaeosomes at a mol % ratio of from 100:0 to 30:70 (sulfated glycolipid: uncharged glycolipid) and which induce a protective immune response, including CD8+ and CD4+ T cell responses. Archaeosomes comprising the sulfated glycolipids described have desirable adjuvant properties, particularly when mixed with uncharged glycolipid at a mol % ratio of about 50:50.

公开(公告)号：US10647737B2

公开(公告)日：2020-05-12

申请号：US15325640

申请日：2015-07-10

申请人： National Research Council of Canada

发明人： Dennis M. Whitfield ,  G. Dennis Sprott ,  Lakshmi Krishnan

IPC分类号： C07H15/04 ,  A61K39/00 ,  A61K39/39

摘要： A synthetic charged glycolipid is described comprising a sulfated saccharide group covalently linked to the tree sn-1 hydroxyl group of the glycerol backbone of an archaeal core lipid via a beta linkage. The synthetic charged glycolipids include compounds of formula I wherein n is 0 or 1; R is hydrogen or hydroxyl; and Y is hydrogen or a sulfate group, at least one Y being a sulfate group; and including pharmaceutically acceptable salts thereof. The sulfated glycolipid produces stable archaeosomes at a mol % ratio of from 100:0 to 30:70 (sulfated glycolipid:uncharged glycolipid) and which induce a protective immune response, including CD8+ and CD4+ T cell responses. Archaeosomes comprising the sulfated glycolipids described have desirable adjuvant properties, particularly when mixed with uncharged glycolipid at a mol % ratio of about 50:50.

公开(公告)号：US20140356912A1

公开(公告)日：2014-12-04

申请号：US14444622

申请日：2014-07-28

申请人： National Research Council of Canada ,  University of Ottawa

发明人： Christopher N. Boddy ,  Susan M. Logan ,  Benjamin R. Lundgren ,  Ian C. Schoenhofen ,  Dennis M. Whitfield

IPC分类号： C12P19/26 ,  C12P19/30 ,  C12N15/70

CPC分类号： C12P19/26 ,  C12N15/52 ,  C12N15/70 ,  C12P19/305

摘要： The present invention relates to the the cell-based production of bacterial nonulosonates and their biosynthetic precursors. Specifically, the present invention provides recombinant cells for the production of pseudaminic acid, legionaminic acid, UDP-2,4-diacetamido-2,4,6-trideoxy-□-L-altropyranose, and UDP-2,4-diacetamido-2,4,6-trideoxy-□-D-glucopyranose. Methods for producing the sugars are also provided.

摘要翻译： 本发明涉及细菌非细菌的细胞生产及其生物合成前体。 具体地说，本发明提供了用于制备假胺酸，军团氨酸，UDP-2,4-二乙酰氨基-2,4,6-三脱氧-D-吡喃糖的重组细胞和UDP-2,4-二乙酰氨基-2 ，4,6-三脱氧-D-吡喃葡萄糖。 还提供了生产糖的方法。

公开(公告)号：US10301659B2

公开(公告)日：2019-05-28

申请号：US15354603

申请日：2016-11-17

申请人： National Research Council of Canada ,  University of Ottawa

发明人： Dennis M. Whitfield ,  Susan M. Logan ,  Ian C. Schoenhofen ,  Christopher N. Boddy ,  Benjamin R. Lundgren

IPC分类号： C12P19/26 ,  C12N15/52 ,  C12N15/70 ,  C12P19/30

摘要： The present invention relates to the cell-based production of bacterial nonulosonates and their biosynthetic precursors. Specifically, the present invention provides recombinant cells for the production of pseudaminic acid, legionaminic acid, UDP-2,4-diacetamido-2,4,6-trideoxy-β-L-altropyranose, and UDP-2,4-diacetamido-2,4,6-trideoxy-α-D-glucopyranose. Methods for producing the sugars are also provided.

公开(公告)号：US09512455B2

公开(公告)日：2016-12-06

申请号：US14444622

申请日：2014-07-28

申请人： National Research Council of Canada ,  University of Ottawa

发明人： Christopher N. Boddy ,  Susan M. Logan ,  Benjamin R. Lundgren ,  Ian C. Schoenhofen ,  Dennis M. Whitfield

IPC分类号： C12P19/26 ,  C12P19/30 ,  C12N15/52 ,  C12N15/70

CPC分类号： C12P19/26 ,  C12N15/52 ,  C12N15/70 ,  C12P19/305

摘要： The present invention relates to the cell-based production of bacterial nonulosonates and their biosynthetic precursors. Specifically, the present invention provides recombinant cells for the production of pseudaminic acid, legionaminic acid, UDP-2,4-diacetamido-2,4,6-trideoxy-□-L-altropyranose, and UDP-2,4-diacetamido-2,4,6-trideoxy-□-D-glucopyranose. Methods for producing the sugars are also provided.

摘要翻译： 本发明涉及细菌非麻黄碱及其生物合成前体的细胞生产。 具体地说，本发明提供了用于制备假胺酸，军团氨酸，UDP-2,4-二乙酰氨基-2,4,6-三脱氧-D-吡喃糖的重组细胞和UDP-2,4-二乙酰氨基-2 ，4,6-三脱氧-D-吡喃葡萄糖。 还提供了生产糖的方法。