公开(公告)号：US07205045B2

公开(公告)日：2007-04-17

申请号：US10944457

申请日：2004-09-16

申请人： Nelson R. Holcomb ,  Russell A. Parker ,  Steven M. Lefkowitz

发明人： Nelson R. Holcomb ,  Russell A. Parker ,  Steven M. Lefkowitz

IPC分类号： G11B11/105

CPC分类号： G01N21/278 ,  G01N21/6428 ,  G01N21/6452 ,  Y10T428/26 ,  Y10T428/268

摘要： Calibration devices for optical scanners and methods for their use are provided. The subject devices are characterized by having a polymeric coating with at least one fluorescent agent, where the devices have minimal local and global nonuniformities. The subject device may also include one or more photobleached regions. In using the subject devices, a surface is illuminated with at least one light source, fluorescence data is obtained from the surface and the optical system is calibrated based upon the obtained fluorescence data. The subject invention finds use in a variety of optical scanners, including biopolymeric array optical scanners. Also provided are kits for use in verifying and calibrating optical scanners.

摘要翻译： 提供了用于光学扫描仪的校准装置及其使用方法。 本发明装置的特征在于具有至少一种荧光剂的聚合物涂层，其中装置具有最小的局部和全局不均匀性。 主体装置还可以包括一个或多个照相漂白区域。 在使用目标装置时，用至少一个光源照射表面，从表面获得荧光数据，并且基于获得的荧光数据校准光学系统。 本发明可用于各种光学扫描仪，包括生物聚合物阵列光学扫描仪。 还提供了用于验证和校准光学扫描仪的套件。

公开(公告)号：US06794424B2

公开(公告)日：2004-09-21

申请号：US10008598

申请日：2001-12-04

申请人： Nelson R. Holcomb ,  Russell A. Parker ,  Steven M. Lefkowitz

发明人： Nelson R. Holcomb ,  Russell A. Parker ,  Steven M. Lefkowitz

IPC分类号： G21F110

CPC分类号： G01N21/278 ,  G01N21/6428 ,  G01N21/6452 ,  Y10T428/26 ,  Y10T428/268

摘要： Calibration devices for optical scanners and methods for their use are provided. The subject devices are characterized by having a polymeric coating with at least one fluorescent agent, where the devices have minimal local and global nonuniformities. The subject device may also include one or more photobleached regions. In using the subject devices, a surface is illuminated with at least one light source, fluorescence data is obtained from the surface and the optical system is calibrated based upon the obtained fluorescence data. The subject invention finds use in a variety of optical scanners, including biopolymeric array optical scanners. Also provided are kits for use in verifying and calibrating optical scanners.

摘要翻译： 提供了用于光学扫描仪的校准装置及其使用方法。 本发明装置的特征在于具有至少一种荧光剂的聚合物涂层，其中装置具有最小的局部和全局不均匀性。 主体装置还可以包括一个或多个照相漂白区域。 在使用目标装置时，用至少一个光源照射表面，从表面获得荧光数据，并且基于获得的荧光数据校准光学系统。 本发明可用于各种光学扫描仪，包括生物聚合物阵列光学扫描仪。 还提供了用于验证和校准光学扫描仪的套件。

公开(公告)号：US06927029B2

公开(公告)日：2005-08-09

申请号：US10005577

申请日：2001-12-03

申请人： Steven M. Lefkowitz ,  Daniel B. Roitman ,  Nelson R. Holcomb

发明人： Steven M. Lefkowitz ,  Daniel B. Roitman ,  Nelson R. Holcomb

IPC分类号： G01N33/543 ,  G01N33/545 ,  C12Q1/68 ,  C07H21/04 ,  C12M1/36 ,  C12N11/16

CPC分类号： G01N33/545 ,  G01N33/54393

摘要： The invention provides a solid support for adsorbing a biomolecule. The support comprises a surface coating having a non-nucleotidic polymer tethered to a surface reactive site. The polymer comprises a backbone, terminus, and adsorbing moieties covalently attached to the backbone and capable of adsorbing a biomolecule that can assume a plurality of conformations. The polymer is generally tethered to the surface at its terminus and the backbone exhibits sufficient mobility and flexibility such that a biomolecule adsorbed by the adsorbing moieties can assume a desired conformation for hybridization. Also provided is a process for preparing a surface coating having a functionalized surface.

摘要翻译： 本发明提供了用于吸附生物分子的固体支持物。 载体包括具有与表面反应性位点连接的非核酸聚合物的表面涂层。 聚合物包含主链，末端和吸附部分，其共价连接到骨架上并且能够吸附可呈现多个构象的生物分子。 聚合物通常在其末端处连接到表面，并且主链显示出足够的迁移率和柔性，使得被吸附部分吸附的生物分子可以呈现出期望的构象用于杂交。 还提供了制备具有官能化表面的表面涂层的方法。

公开(公告)号：US06753145B2

公开(公告)日：2004-06-22

申请号：US09900084

申请日：2001-07-05

申请人： Nelson R. Holcomb ,  Patrick J. Collins ,  Karen W. Shannon ,  Steven M. Lefkowitz

发明人： Nelson R. Holcomb ,  Patrick J. Collins ,  Karen W. Shannon ,  Steven M. Lefkowitz

IPC分类号： C12Q168

CPC分类号： C12Q1/689

摘要： A buffer composition, method and kit for hybridizing microarrays of nucleic acids bound to an adsorbed polymer surface of a siliceous substrate provide an envelope of conditions to hybridize nucleic acid targets, while preserving theintactness of the adsorbed polymer surface of the array. The buffer composition comprises a non-chelating buffering agent, a pH within a range of pH 6.4 and 7.5, a monovalent cation having a monovalent cation concentration that ranges from about 0.01 M to about 2.0 M, and optionally relatively lower concentrations of a chelating agent and an ionic surfactant. The total cation concentration of the buffer composition ranges from about 0.02 M to about 2.0 M. The method comprises incubating the targets with the microarray in the buffer composition at a temperature between about 55° C. and 70° C.

摘要翻译： 用于杂交与硅质底物的吸附的聚合物表面结合的核酸的微阵列的缓冲组合物，方法和试剂盒提供了与核酸靶标杂交的条件的包络线，同时保持阵列的吸附的聚合物表面的活性。 缓冲剂组合物包含非螯合缓冲剂，pH在6.4和7.5范围内的pH，一价阳离子浓度范围为约0.01M至约2.0M，任选相对较低浓度的螯合剂 和离子表面活性剂。 缓冲剂组合物的总阳离子浓度范围为约0.02M至约2.0M。该方法包括在约55℃至70℃的温度下将靶标与缓冲液组合物中的微阵列温育。

公开(公告)号：US20080241892A1

公开(公告)日：2008-10-02

申请号：US12079922

申请日：2008-03-27

申请人： Daniel Bernardo Roitman ,  Geoff Otto ,  Ronald L. Cicero ,  Nelson R. Holcomb

发明人： Daniel Bernardo Roitman ,  Geoff Otto ,  Ronald L. Cicero ,  Nelson R. Holcomb

IPC分类号： C12P19/34 ,  B32B15/08 ,  C40B50/14 ,  C40B50/06 ,  B32B27/00 ,  C12P1/00 ,  C07H21/00 ,  C12N11/14

CPC分类号： C12N11/14 ,  C07B2200/11 ,  C40B50/14 ,  Y10T428/31504 ,  Y10T428/31678

摘要： Modified surfaces, substrates, and methods of producing and using such substrates and surfaces are provided. The substrates and surfaces provide either non-reactive surfaces or low density reactive groups, preferably on an otherwise non-reactive surface, for use in different applications including single molecule analyses.

摘要翻译： 提供了改进的表面，基底以及制造和使用这些基底和表面的方法。 衬底和表面提供非反应性表面或低密度反应性基团，优选在非反应性表面上，用于不同应用，包括单分子分析。