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    Devices for calibrating optical scanners and methods of using the same
    1.
    发明授权
    Devices for calibrating optical scanners and methods of using the same 失效
    用于校准光学扫描仪的设备及其使用方法

    公开(公告)号：US06794424B2

    公开(公告)日：2004-09-21

    申请号：US10008598

    申请日：2001-12-04

    申请人： Nelson R. Holcomb ,  Russell A. Parker ,  Steven M. Lefkowitz

    发明人： Nelson R. Holcomb ,  Russell A. Parker ,  Steven M. Lefkowitz

    IPC分类号： G21F110

    CPC分类号： G01N21/278 ,  G01N21/6428 ,  G01N21/6452 ,  Y10T428/26 ,  Y10T428/268

    摘要： Calibration devices for optical scanners and methods for their use are provided. The subject devices are characterized by having a polymeric coating with at least one fluorescent agent, where the devices have minimal local and global nonuniformities. The subject device may also include one or more photobleached regions. In using the subject devices, a surface is illuminated with at least one light source, fluorescence data is obtained from the surface and the optical system is calibrated based upon the obtained fluorescence data. The subject invention finds use in a variety of optical scanners, including biopolymeric array optical scanners. Also provided are kits for use in verifying and calibrating optical scanners.

    摘要翻译： 提供了用于光学扫描仪的校准装置及其使用方法。 本发明装置的特征在于具有至少一种荧光剂的聚合物涂层，其中装置具有最小的局部和全局不均匀性。 主体装置还可以包括一个或多个照相漂白区域。 在使用目标装置时，用至少一个光源照射表面，从表面获得荧光数据，并且基于获得的荧光数据校准光学系统。 本发明可用于各种光学扫描仪，包括生物聚合物阵列光学扫描仪。 还提供了用于验证和校准光学扫描仪的套件。

    Devices for calibrating optical scanners and methods of using the same
    2.
    发明授权
    Devices for calibrating optical scanners and methods of using the same 失效
    用于校准光学扫描仪的设备及其使用方法

    公开(公告)号：US07205045B2

    公开(公告)日：2007-04-17

    申请号：US10944457

    申请日：2004-09-16

    申请人： Nelson R. Holcomb ,  Russell A. Parker ,  Steven M. Lefkowitz

    发明人： Nelson R. Holcomb ,  Russell A. Parker ,  Steven M. Lefkowitz

    IPC分类号： G11B11/105

    CPC分类号： G01N21/278 ,  G01N21/6428 ,  G01N21/6452 ,  Y10T428/26 ,  Y10T428/268

    摘要： Calibration devices for optical scanners and methods for their use are provided. The subject devices are characterized by having a polymeric coating with at least one fluorescent agent, where the devices have minimal local and global nonuniformities. The subject device may also include one or more photobleached regions. In using the subject devices, a surface is illuminated with at least one light source, fluorescence data is obtained from the surface and the optical system is calibrated based upon the obtained fluorescence data. The subject invention finds use in a variety of optical scanners, including biopolymeric array optical scanners. Also provided are kits for use in verifying and calibrating optical scanners.

    摘要翻译： 提供了用于光学扫描仪的校准装置及其使用方法。 本发明装置的特征在于具有至少一种荧光剂的聚合物涂层，其中装置具有最小的局部和全局不均匀性。 主体装置还可以包括一个或多个照相漂白区域。 在使用目标装置时，用至少一个光源照射表面，从表面获得荧光数据，并且基于获得的荧光数据校准光学系统。 本发明可用于各种光学扫描仪，包括生物聚合物阵列光学扫描仪。 还提供了用于验证和校准光学扫描仪的套件。

    Surface with tethered polymeric species for binding biomolecules
    3.
    发明授权
    Surface with tethered polymeric species for binding biomolecules 失效
    具有束缚聚合物的表面用于结合生物分子

    公开(公告)号：US06927029B2

    公开(公告)日：2005-08-09

    申请号：US10005577

    申请日：2001-12-03

    申请人： Steven M. Lefkowitz ,  Daniel B. Roitman ,  Nelson R. Holcomb

    发明人： Steven M. Lefkowitz ,  Daniel B. Roitman ,  Nelson R. Holcomb

    IPC分类号： G01N33/543 ,  G01N33/545 ,  C12Q1/68 ,  C07H21/04 ,  C12M1/36 ,  C12N11/16

    CPC分类号： G01N33/545 ,  G01N33/54393

    摘要： The invention provides a solid support for adsorbing a biomolecule. The support comprises a surface coating having a non-nucleotidic polymer tethered to a surface reactive site. The polymer comprises a backbone, terminus, and adsorbing moieties covalently attached to the backbone and capable of adsorbing a biomolecule that can assume a plurality of conformations. The polymer is generally tethered to the surface at its terminus and the backbone exhibits sufficient mobility and flexibility such that a biomolecule adsorbed by the adsorbing moieties can assume a desired conformation for hybridization. Also provided is a process for preparing a surface coating having a functionalized surface.

    摘要翻译： 本发明提供了用于吸附生物分子的固体支持物。 载体包括具有与表面反应性位点连接的非核酸聚合物的表面涂层。 聚合物包含主链，末端和吸附部分，其共价连接到骨架上并且能够吸附可呈现多个构象的生物分子。 聚合物通常在其末端处连接到表面，并且主链显示出足够的迁移率和柔性，使得被吸附部分吸附的生物分子可以呈现出期望的构象用于杂交。 还提供了制备具有官能化表面的表面涂层的方法。

    Buffer composition and method for hybridization of microarrays on adsorbed polymer siliceous surfaces
    4.
    发明授权
    Buffer composition and method for hybridization of microarrays on adsorbed polymer siliceous surfaces 失效
    缓冲液组合物和微阵列在吸附聚合物硅质表面上的杂交方法

    公开(公告)号：US06753145B2

    公开(公告)日：2004-06-22

    申请号：US09900084

    申请日：2001-07-05

    申请人： Nelson R. Holcomb ,  Patrick J. Collins ,  Karen W. Shannon ,  Steven M. Lefkowitz

    发明人： Nelson R. Holcomb ,  Patrick J. Collins ,  Karen W. Shannon ,  Steven M. Lefkowitz

    IPC分类号： C12Q168

    CPC分类号： C12Q1/689

    摘要： A buffer composition, method and kit for hybridizing microarrays of nucleic acids bound to an adsorbed polymer surface of a siliceous substrate provide an envelope of conditions to hybridize nucleic acid targets, while preserving theintactness of the adsorbed polymer surface of the array. The buffer composition comprises a non-chelating buffering agent, a pH within a range of pH 6.4 and 7.5, a monovalent cation having a monovalent cation concentration that ranges from about 0.01 M to about 2.0 M, and optionally relatively lower concentrations of a chelating agent and an ionic surfactant. The total cation concentration of the buffer composition ranges from about 0.02 M to about 2.0 M. The method comprises incubating the targets with the microarray in the buffer composition at a temperature between about 55° C. and 70° C.

    摘要翻译： 用于杂交与硅质底物的吸附的聚合物表面结合的核酸的微阵列的缓冲组合物，方法和试剂盒提供了与核酸靶标杂交的条件的包络线，同时保持阵列的吸附的聚合物表面的活性。 缓冲剂组合物包含非螯合缓冲剂，pH在6.4和7.5范围内的pH，一价阳离子浓度范围为约0.01M至约2.0M，任选相对较低浓度的螯合剂 和离子表面活性剂。 缓冲剂组合物的总阳离子浓度范围为约0.02M至约2.0M。该方法包括在约55℃至70℃的温度下将靶标与缓冲液组合物中的微阵列温育。

    Method of shielding biosynthesis reactions from the ambient environment on an array
    6.
    发明授权
    Method of shielding biosynthesis reactions from the ambient environment on an array 失效

    公开(公告)号：US06387636B1

    公开(公告)日：2002-05-14

    申请号：US09426823

    申请日：1999-10-22

    申请人： Michel G. M. Perbost ,  Steven M. Lefkowitz

    发明人： Michel G. M. Perbost ,  Steven M. Lefkowitz

    IPC分类号： G01N3353

    CPC分类号： B01J19/0046 ,  B01J2219/00378 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00599 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00698 ,  B01J2219/00722 ,  C40B40/06 ,  C40B50/08 ,  C40B60/14

    摘要： A method of fabricating an array of biopolymers provides a shield for biochemical reactions and biochemical reactants and is particularly useful for those reactions and reactants that are susceptible to reaction with a component of the ambient environment during the fabrication of the array. The method is applicable to the conventional fabrication and synthesis methods used to fabricate a biopolymer array, such as in situ synthesis of biopolymers on an array and the attachment of pre-synthesized biopolymers on to an array. The method comprises applying a non-miscible fluid (NMF) to the array surface where the biopolymers are being synthesized or attached. The NMF is inert and insoluble with the biochemical reactants and other ancillary materials in solution used in conventional synthesis or attachment of biopolymers. The NMF provides a shield between the ambient atmosphere and the biopolymer synthesis materials or the deprotected pre-synthesized biopolymer at the surface of the array during the synthesis or attachment processes. The NMF may be applied as droplets over each feature location on the surface or may be applied by flooding the surface of the array to fully cover the features. Biomonomer or biopolymer solutions are deposited into or through the NMF to the feature locations on the surface of the array where the synthesis or attachment reactions are to take place using conventional deposition equipment to eject the solutions into the NMF. The NMF provides a shield for activated biomonomers that are susceptible to reaction with a component in the ambient environment, such as moisture in the air. Moreover, the NMF provides a shield for pre-synthesized biopolymers that are susceptible to evaporation when deprotected for attachment to the array surface. The method provides a means by which the potential reactivity of the activated biomonomer or deprotected biopolymer with an ambient atmosphere component can be kept low. As a result, biopolymer arrays can be more accurately fabricated.

    Methods for attaching substances to surfaces
    7.
    发明授权
    Methods for attaching substances to surfaces 失效
    将物质附着于表面的方法

    公开(公告)号：US06319674B1

    公开(公告)日：2001-11-20

    申请号：US09397527

    申请日：1999-09-16

    申请人： Geraldine Fulcrand ,  Douglas J. Dellinger ,  Steven M. Lefkowitz

    发明人： Geraldine Fulcrand ,  Douglas J. Dellinger ,  Steven M. Lefkowitz

    IPC分类号： G01N3353

    CPC分类号： G01N33/54353

    摘要： Methods are disclosed for immobilizing a substance to a surface. A surface is employed that comprises a linking group consisting of a first portion comprising a hydrocarbon chain, optionally substituted, and a second portion comprising an alkylene oxide or an alkylene imine wherein the alkylene is optionally substituted. One end of the first portion is attached to the surface and one end of the second portion is attached to the other end of the first portion chain by means of an amine or an oxy functionality. The second portion terminates in an amine or a hydroxy functionality. The surface is reacted with the substance to be immobilized under conditions for attachment of the substance to the surface by means of the linking group. Compositions of matter and reaction systems are also disclosed.

    摘要翻译： 公开了将物质固定在表面上的方法。 使用表面，其包含由包含任选取代的烃链的第一部分和包含烯化氧或亚烷基亚胺的第二部分组成的连接基团，其中所述亚烷基是任选取代的。 第一部分的一端附接到表面，并且第二部分的一端通过胺或氧官能团连接到第一部分链的另一端。 第二部分终止于胺或羟基官能团。 在通过连接基团将物质附着到表面的条件下，表面与要固定的物质反应。 还公开了物质和反应体系的组成。