公开(公告)号：US12173368B2

公开(公告)日：2024-12-24

申请号：US18298291

申请日：2023-04-10

Applicant: New England Biolabs, Inc.

Inventor： Ivan R. Correa, Jr. ,  Eric Wolf ,  Nan Dai ,  Erbay Yigit ,  Sebastian Grünberg

IPC: C12Q1/6806 ,  C12Q1/6872

Abstract: The present disclosure relates, according to some embodiments, to compositions and analysis of RNA (e.g., dephosphorylated oligoribonucleotides) including, for example, natural and/or synthetic RNAs. A composition may comprise, for example, an endoribonuclease having an amino acid sequence that (i) corresponds to an amino acid sequence of a first species (e.g., Homo sapiens, Escherichia coli, Aspergillus oryzae, Momordica charantia, Pyrococcus furiosus, Cucumis sativus, and Sus scrofa) or (ii) is a non-naturally occurring sequence; and/or an RNA end repair enzyme having an amino acid sequence that (i) corresponds to an amino acid sequence of a species other than the first species (e.g., a bacterial species or a bacteriophage species) or (ii) is a non-naturally occurring sequence.

公开(公告)号：US20240301485A1

公开(公告)日：2024-09-12

申请号：US18660998

申请日：2024-05-10

Applicant: New England Biolabs, Inc.

Inventor： Ivan R. Correa, JR. ,  Eric Wolf ,  Nan Dai ,  Erbay Yigit ,  Sebastian Grünberg

IPC: C12Q1/6872 ,  C12Q1/6806

CPC classification number: C12Q1/6872 ,  C12Y301/13005 ,  C12Q1/6806 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: The present disclosure relates, according to some embodiments, to compositions and analysis of RNA (e.g., dephosphorylated oligoribonucleotides) including, for example, natural and/or synthetic RNAs. A composition may comprise, for example, an endoribonuclease having an amino acid sequence that (i) corresponds to an amino acid sequence of a first species (e.g., Homo sapiens, Escherichia coli, Aspergillus oryzae, Momordica charantia, Pyrococcus furiosus, Cucumis sativus, and Sus scrofa) or (ii) is a non-naturally occurring sequence; and/or an RNA end repair enzyme having an amino acid sequence that (i) corresponds to an amino acid sequence of a species other than the first species (e.g., a bacterial species or a bacteriophage species) or (ii) is a non-naturally occurring sequence.

公开(公告)号：US20230287489A1

公开(公告)日：2023-09-14

申请号：US18298291

申请日：2023-04-10

Applicant: New England Biolabs, Inc.

Inventor： Ivan R. Correa, Jr. ,  Eric Wolf ,  Nan Dai ,  Erbay Yigit ,  Sebastian Grünberg

IPC: C12Q1/6872

CPC classification number: C12Q1/6872 ,  C12Q1/6806

Abstract: The present disclosure relates, according to some embodiments, to compositions and analysis of RNA (e.g., dephosphorylated oligoribonucleotides) including, for example, natural and/or synthetic RNAs. A composition may comprise, for example, an endoribonuclease having an amino acid sequence that (i) corresponds to an amino acid sequence of a first species (e.g., Homo sapiens, Escherichia coli, Aspergillus oryzae, Momordica charantia, Pyrococcus furiosus, Cucumis sativus, and Sus scrofa) or (ii) is a non-naturally occurring sequence; and/or an RNA end repair enzyme having an amino acid sequence that (i) corresponds to an amino acid sequence of a species other than the first species (e.g., a bacterial species or a bacteriophage species) or (ii) is a non-naturally occurring sequence.