公开(公告)号：US5424395A

公开(公告)日：1995-06-13

申请号：US937236

申请日：1992-08-28

申请人： Newell F. Bascomb ,  Claudio Mapelli ,  Michael D. Swerdloff ,  Jon I. Williams ,  Nicholas P. Everett

发明人： Newell F. Bascomb ,  Claudio Mapelli ,  Michael D. Swerdloff ,  Jon I. Williams ,  Nicholas P. Everett

IPC分类号： G01N33/15 ,  A01N37/20 ,  A01N63/00 ,  A01N63/02 ,  A61K38/00 ,  A61P31/04 ,  C07H21/04 ,  C07K14/46 ,  C12N15/05 ,  C12N15/09 ,  C12N15/12 ,  C12N15/82 ,  C12P21/02 ,  C12R1/19 ,  C07K5/00 ,  C07K7/00 ,  C07K17/00

CPC分类号： A01N63/02 ,  C07K14/46 ,  C12N15/8281 ,  C12N15/8282

摘要： The present invention relates to a number of peptides which have antimicrobial activity and which are useful in retarding plant pathogens. In addition, compounds in accordance with the present invention may have improved resistance to degradation by plant proteases, and/or sufficiently low phytotoxicity to render them likely candidates for use in conjunction with plants. The present invention also relates to oligonucleotides which are capable of expressing the aforementioned peptides. Also provided hereby is a process of retarding the growth of plant pathogens using the compounds of the present invention as well as a screening method and screening reagent useful in determining the proteolytic degradation resistance of a known compound.

摘要翻译： 本发明涉及许多具有抗微生物活性并可用于延缓植物病原体的肽。 此外，根据本发明的化合物可具有改善的抗植物蛋白酶降解性和/或足够低的植物毒性，使其成为可能与植物结合使用的候选物。 本发明还涉及能够表达上述肽的寡核苷酸。 此处也提供了使用本发明化合物延缓植物病原体生长的方法以及可用于测定已知化合物的蛋白水解降解抗性的筛选方法和筛选试剂。

公开(公告)号：US5948612A

公开(公告)日：1999-09-07

申请号：US413618

申请日：1995-03-30

申请人： Newell F. Bascomb ,  Sandra D. Carson

发明人： Newell F. Bascomb ,  Sandra D. Carson

IPC分类号： C12N1/21 ,  C12N9/00 ,  C12N9/88 ,  C12N15/09 ,  C12Q1/02 ,  C12Q1/48 ,  C12R1/19 ,  C12Q1/68 ,  C12Q1/18

CPC分类号： C12N9/00 ,  C12N9/88 ,  C12Q1/025 ,  G01N2430/20

摘要： This invention pertains to novel protocols for the screening and rapid identification of compounds that specifically inhibit a predetermined enzyme or metabolic target site or pathway that is specific to plants. Enzymes which are specifically or indirected affected by the novel screens include glutamine synthetase (GS), 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DAHP), dihydrodipicolinate synthase (DHPS), acetohydroxyacid synthase (AHAS) and phosphoribosyl anthranilate transferase (PAT). The enzymatic pathways targeted by the novel screening protocols are unique to plants, bacteria and fungi and are present at low levels. Thus, inhibiting these enzymes should present little or no toxicity to humans or animals. The screens provide an efficient and rapid method for assessing the herbicidal potential of test compounds. Lead compounds identified by the novel screening protocols can be used as herbicides to inhibit growth.

摘要翻译： 本发明涉及用于筛选和快速鉴定特异性抑制植物特异性的预定酶或代谢靶位点或途径的化合物的新方案。 受新颖筛选影响的酶特异或间接的酶包括谷氨酰胺合成酶（GS），3-脱氧-D-阿拉伯 - 庚酮酸7-磷酸合成酶（DAHP），二氢吡啶二酸合成酶（DHPS），乙酰羟酸合酶（AHAS）和磷酸核糖基邻氨基苯甲酸转移酶 （拍）。 新的筛选方案所针对的酶途径对于植物，细菌和真菌来说是独一无二的，并且以低水平存在。 因此，抑制这些酶对人或动物几乎没有或没有毒性。 屏幕提供了用于评估测试化合物的除草潜力的有效和快速的方法。 通过新的筛选方案鉴定的铅化合物可用作除草剂抑制生长。