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    Compositions and Methods for Increasing Plant Tolerance to High Population Density
    1.
    发明申请
    Compositions and Methods for Increasing Plant Tolerance to High Population Density 有权
    增加植物对高群体密度的耐受性的组合和方法

    公开(公告)号：US20080235827A1

    公开(公告)日：2008-09-25

    申请号：US11682909

    申请日：2007-03-07

    申请人： Nicholas J. Bate ,  Milo J. Aukerman

    发明人： Nicholas J. Bate ,  Milo J. Aukerman

    IPC分类号： C12N15/82 ,  C12N15/00 ,  C12N15/11 ,  A01H5/00

    CPC分类号： C12N15/8261 ,  C07K14/415 ,  Y02A40/146

    摘要： Compositions and methods for suppressing the shade-avoidance response of plants and improving plant yield are provided. Compositions of the invention include an early flowering 3 (ELF3) maize gene, the promoter for this gene, an Arabidopsis basic helix-loop-helix transcription factor (bHLH-041), and fragments and variants thereof. The ELF3 promoter sequence is useful for driving expression of polynucleotides of interest in a plant. The ELF3 and bHLH-041 sequences of the invention, or variants and fragments thereof, are provided in expression cassettes for use in manipulating expression of the ELF3 and bHLH-041 genes. By increasing expression of ELF3 and/or suppressing expression of bHLH-041, the methods of the invention provide for altered response of a plant to light quality and suppression of the high-density-invoked survival mode of development. The invention thus provides methods for growing crop plants at high population densities for yield enhancement. Transformed plants having the altered shade-avoidance phenotype of the invention, and seeds of said plants, are also provided.

    摘要翻译： 提供了抑制植物的避光反应和提高植物产量的组合物和方法。 本发明的组合物包括早期开花3（ELF3）玉米基因，该基因的启动子，拟南芥碱性螺旋 - 环 - 螺旋转录因子（bHLH-041）及其片段和变体。 ELF3启动子序列可用于驱动植物中感兴趣多核苷酸的表达。 在用于操纵ELF3和bHLH-041基因表达的表达盒中提供本发明的ELF3和bHLH-041序列或其变体和片段。 通过增加ELF3的表达和/或抑制bHLH-041的表达，本发明的方法提供植物对光质量的响应改变和抑制高密度诱导的存活发育模式。 因此，本发明提供了用于以高群体密度种植作物以提高产量的方法。 还提供了具有本发明的遮荫避免表型和所述植物的种子的转化植物。

    Compositions and Methods for Increasing Plant Tolerance to High Population Density
    2.
    发明申请
    Compositions and Methods for Increasing Plant Tolerance to High Population Density 审中-公开
    增加植物对高群体密度的耐受性的组合和方法

    公开(公告)号：US20110078824A1

    公开(公告)日：2011-03-31

    申请号：US12960734

    申请日：2010-12-06

    申请人： Nicholas J. Bate ,  Milo J. Aukerman

    发明人： Nicholas J. Bate ,  Milo J. Aukerman

    IPC分类号： C12N15/82 ,  C07H21/00 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12N15/8261 ,  C07K14/415 ,  Y02A40/146

    摘要： Compositions and methods for suppressing the shade-avoidance response of plants and improving plant yield are provided. Compositions of the invention include an early flowering 3 (ELF3) maize gene, the promoter for this gene, an Arabidopsis basic helix-loop-helix transcription factor (bHLH-041), and fragments and variants thereof. The ELF3 promoter sequence is useful for driving expression of polynucleotides of interest in a plant. The ELF3 and bHLH-041 sequences of the invention, or variants and fragments thereof, are provided in expression cassettes for use in manipulating expression of the ELF3 and bHLH-041 genes. By increasing expression of ELF3 and/or suppressing expression of bHLH-041, the methods of the invention provide for altered response of a plant to light quality and suppression of the high-density-invoked survival mode of development. The invention thus provides methods for growing crop plants at high population densities for yield enhancement. Transformed plants having the altered shade-avoidance phenotype of the invention, and seeds of said plants, are also provided.

    摘要翻译： 提供了抑制植物的避光反应和提高植物产量的组合物和方法。 本发明的组合物包括早期开花3（ELF3）玉米基因，该基因的启动子，拟南芥碱性螺旋 - 环 - 螺旋转录因子（bHLH-041）及其片段和变体。 ELF3启动子序列可用于驱动植物中感兴趣多核苷酸的表达。 在用于操纵ELF3和bHLH-041基因表达的表达盒中提供本发明的ELF3和bHLH-041序列或其变体和片段。 通过增加ELF3的表达和/或抑制bHLH-041的表达，本发明的方法提供植物对光质量的响应改变和抑制高密度诱导的存活发育模式。 因此，本发明提供了用于以高群体密度种植作物以提高产量的方法。 还提供了具有本发明的遮荫避免表型和所述植物的种子的转化植物。

    Gene silencing
    3.
    发明授权
    Gene silencing 有权
    基因沉默

    公开(公告)号：US08729339B2

    公开(公告)日：2014-05-20

    申请号：US11958423

    申请日：2007-12-18

    申请人： Milo J. Aukerman

    发明人： Milo J. Aukerman

    IPC分类号： C12N15/82 ,  C07H21/04

    CPC分类号： C12N15/8201 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/827

    摘要： The invention provides methods and compositions useful in target sequence suppression and target sequence validation. The invention provides polynucleotide constructs useful for gene silencing, as well as cells, plants and seeds comprising the polynucleotides. The invention also provides a method for using microRNA to silence a target sequence.

    摘要翻译： 本发明提供了可用于靶序列抑制和靶序列验证的方法和组合物。 本发明提供了可用于基因沉默的多核苷酸构建体，以及包含多核苷酸的细胞，植物和种子。 本发明还提供了使用微小RNA沉默靶序列的方法。

    Gene Silencing
    7.
    发明申请
    Gene Silencing 有权
    基因沉默

    公开(公告)号：US20120144527A1

    公开(公告)日：2012-06-07

    申请号：US11958423

    申请日：2007-12-18

    申请人： Milo J. Aukerman

    发明人： Milo J. Aukerman

    IPC分类号： C12N15/82 ,  A01H5/10 ,  A01H5/00 ,  C12N5/10 ,  C12N15/63

    CPC分类号： C12N15/8201 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/827

    摘要： The invention provides methods and compositions useful in target sequence suppression and target sequence validation. The invention provides polynucleotide constructs useful for gene silencing, as well as cells, plants and seeds comprising the polynucleotides. The invention also provides a method for using microRNA to silence a target sequence.

    摘要翻译： 本发明提供了可用于靶序列抑制和靶序列验证的方法和组合物。 本发明提供了可用于基因沉默的多核苷酸构建体，以及包含多核苷酸的细胞，植物和种子。 本发明还提供了使用微小RNA沉默靶序列的方法。